LOCUS       sequence-282527-        2867 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1069
FEATURES             Location/Qualifiers
     source          1..2867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2176..2185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgaacgcccc cgagcggcag ccccaacccg acggcgggga cgccccaggc
       61 cacgagcctg ggggcagccc ccaagacgag cttgacttct ccatcctctt cgactatgag
      121 tatttgaatc cgaacgaaga agagccgaat gcacataagg tcgccagccc accctccgga
      181 cccgcatacc ccgatgatgt cctggactat ggcctcaagc catacagccc ccttgctagt
      241 ctctctggcg agccccccgg ccgattcgga gagccggata gggtagggcc gcagaagttt
      301 ctgagcgcgg ccaagccagc aggggcctcg ggcctgagcc ctcggatcga gatcactccg
      361 tcccacgaac tgatccaggc agtggggccc ctccgcatga gagacgcggg cctcctggtg
      421 gagcagccgc ccctggccgg ggtggccgcc agcccgaggt tcaccctgcc cgtgcccggc
      481 ttcgagggct accgcgagcc gctttgcttg agccccgcta gcagcggctc ctctgccagc
      541 ttcatttctg acaccttctc cccctacacc tcgccctgcg tctcgcccaa taacggcggg
      601 cccgacgacc tgtgtccgca gtttcaaaac atccctgctc attattcccc cagaacctcg
      661 ccaataatgt cacctcgaac cagcctcgcc gaggacagct gcctgggccg ccactcgccc
      721 gtgccccgtc cggcctcccg ctcctcatcg cctggtgcca agcggaggca ttcgtgcgcc
      781 gaggccttgg ttgccctgcc gcccggagcc tcaccccagc gctcccggag cccctcgccg
      841 cagccctcat ctcacgtggc accccaggac cacggctccc cggctgggta cccccctgtg
      901 gctggctctg ccgtgatcat ggatgccctg aacagcctcg ccacggactc gccttgtggg
      961 atccccccca agatgtggaa gaccagccct gacccctcgc cggtgtctgc cgccccatcc
     1021 aaggccggcc tgcctcgcca catctacccg gccgtggagt tcctggggcc ctgcgagcag
     1081 ggcgagagga gaaactcggc tccagaatcc atcctgctgg ttccgcccac ttggcccaag
     1141 ccgctggtgc ctgccattcc catctgcagc atcccagtga ctgcatccct ccctccactt
     1201 gagtggccgc tgtccagtca gtcaggctct tacgagctgc ggatcgaggt gcagcccaag
     1261 ccacatcacc gggcccacta tgagacagaa ggcagccgag gggctgtcaa agctccaact
     1321 ggaggccacc ctgtggttca gctccatggc tacatggaaa acaagcctct gggacttcag
     1381 atcttcattg ggacagctga tgagcggatc cttaagccgc acgccttcta ccaggtgcac
     1441 cgaatcacgg ggaaaactgt caccaccacc agctatgaga agatagtggg caacaccaaa
     1501 gtcctggaga tacccttgga gcccaaaaac aacatgaggg caaccatcga ctgtgcgggg
     1561 atcttgaagc ttagaaacgc cgacattgag ctgcggaaag gcgagacgga cattggaaga
     1621 aagaacacgc gggtgagact ggttttccga gttcacatcc cagagtccag tggcagaatc
     1681 gtctctttac agactgcatc taaccccatc gagtgctccc agcgatctgc tcacgagctg
     1741 cccatggttg aaagacaaga cacagacagc tgcctggtct atggcggcca gcaaatgatc
     1801 ctcacggggc agaactttac atccgagtcc aaagttgtgt ttactgagaa gaccacagat
     1861 ggacagcaaa tttgggagat ggaagccacg gtggataagg acaagagcca gcccaacatg
     1921 ctttttgttg agatccctga atatcggaac aagcatatcc gcacacctgt aaaagtgaac
     1981 ttctacgtca tcaatgggaa gagaaaacga agtcagcctc agcactttac ctaccaccca
     2041 gtcccagcca tcaagacgga gcccacggat gaatatgacc ccactctgat ctgcagcccc
     2101 acccatggag gcctggggag ccagccttac tacccccagc acccgatggt ggccgagtcc
     2161 ccctcctgcc tcgtggccac catggctccc tgccagcagt tccgcacggg gctctcatcc
     2221 cctgacgccc gctaccagca acagaaccca gcggccgtac tctaccagcg gagcaagagc
     2281 ctgagcccca gcctgctggg ctatcagcag ccggccctca tggccgcccc gctgtccctt
     2341 gcggacgctc accgctctgt gctggtgcac gccggctccc agggccagag ctcagccctg
     2401 ctccacccct ctccgaccaa ccagcaggcc tcgcctgtga tccactactc acccaccaac
     2461 cagcagctgc gctgcggaag ccaccaggag ttccagcaca tcatgtactg cgagaatttc
     2521 gcaccaggca ccaccagacc tggcccgccc ccggtcagtc aaggtcagag gctgagcccg
     2581 ggttcctacc ccacagtcat tcagcagcag aatgccacga gccaaagagc cgccaaaaac
     2641 ggacccccgg tcagtgacca aaaggaagta ttacctgcgg gggtgaccat taaacaggag
     2701 cagaacttgg accagaccta cttggatgat gagctgatag acacacacct tagctggata
     2761 caaaacatat tatagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2821 tatatatctt gtggaaagga cgacctgtcg tataacctat gtgagac
//
