LOCUS       sequence-282564-        1709 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1181
FEATURES             Location/Qualifiers
     source          1..1709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggcatcgt ggagccgggt tgcggagaca tgctgacggg caccgagccg
       61 atgccgggga gcgacgaggg ccgggcgcct ggcgccgacc cgcagcaccg ctacttctac
      121 ccggagccgg gcgcgcagga cgcggacgag cgtcgcgggg gcggcagcct ggggtctccc
      181 tacccggggg gcgccttggt gcccgccccg ccgagccgct tccttggagc ctacgcctac
      241 ccgccgcgac cccaggcggc cggcttcccc ggcgcgggcg agtccttccc gccgcccgcg
      301 gacgccgagg gctaccagcc gggcgagggc tacgccgccc cggacccgcg cgccgggctc
      361 tacccggggc cgcgtgagga ctacgcgcta cccgcgggac tggaggtgtc ggggaaactg
      421 agggtcgcgc tcaacaacca cctgttgtgg tccaagttta atcagcacca gacagagatg
      481 atcatcacca agcagggacg gcggatgttc ccattcctgt catttactgt ggccgggctg
      541 gagcccacca gccactacag gatgtttgtg gacgtggtct tggtggacca gcaccactgg
      601 cggtaccaga gcggcaagtg ggtgcagtgt ggaaaggccg agggcagcat gccaggaaac
      661 cgcctgtacg tccacccgga ctcccccaac acaggagcgc actggatgcg ccaggaagtt
      721 tcatttggga aactaaagct cacaaacaac aagggggcgt ccaacaatgt gacccagatg
      781 attgtgctcc agtccctcca taagtaccag ccccggctgc atatcgttga ggtgaacgac
      841 ggagagccag aggcagcctg caacgcttcc aacacgcata tctttacttt ccaagaaacc
      901 cagttcattg ccgtgactgc ctaccagaat gccgagatta ctcagctgaa aattgataat
      961 aacccctttg ccaaaggatt ccgggagaac tttgagtcca tgtacacatc tgttgacacc
     1021 agcatcccct ccccgcctgg acccaactgt caattccttg ggggagatca ctactctcct
     1081 ctcctaccca accagtatcc tgttcccagc cgcttctacc ccgaccttcc tggccaggcg
     1141 aaggatgtgg ttccccaggc ttactggctg ggggcccccc gggaccacag ctatgaggct
     1201 gagtttcgag cagtcagcat gaagcctgca ttcttgccct ctgcccctgg gcccaccatg
     1261 tcctactacc gaggccagga ggtcctggca cctggagctg gctggcctgt ggcaccccag
     1321 taccctccca agatgggccc ggccagctgg ttccgcccta tgcggactct gcccatggaa
     1381 cccggccctg gaggctcaga gggacgggga ccagaggacc agggtccccc cttggtgtgg
     1441 actgagattg cccccatccg gccggaatcc agtgattcag gactgggcga aggagactct
     1501 aagaggaggc gcgtgtcccc ctatccttcc agtggtgaca gctcctcccc tgctggggcc
     1561 ccttctcctt ttgataagga agctgaagga cagttttata actattttcc caactagtaa
     1621 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1681 gacgacgggc atttaagcgc tcagtggtt
//
