LOCUS       sequence-282624-        2330 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1433
FEATURES             Location/Qualifiers
     source          1..2330
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggactccaa agaatcatta actcctggta gagaagaaaa ccccagcagt
       61 gtgcttgctc aggagagggg agatgtgatg gacttctata aaaccctaag aggaggagct
      121 actgtgaagg tttctgcgtc ttcaccctca ctggctgtcg cttctcaatc agactccaag
      181 cagcgaagac ttttggttga ttttccaaaa ggctcagtaa gcaatgcgca gcagccagat
      241 ctgtccaaag cagtttcact ctcaatggga ctgtatatgg gagagacaga aacaaaagtg
      301 atgggaaatg acctgggatt cccacagcag ggccaaatca gcctttcctc gggggaaaca
      361 gacttaaagc ttttggaaga aagcattgca aacctcaata ggtcgaccag tgttccagag
      421 aaccccaaga gttcagcatc cactgctgtg tctgctgccc ccacagagaa ggagtttcca
      481 aaaactcact ctgatgtatc ttcagaacag caacatttga agggccagac tggcaccaac
      541 ggtggcaatg tgaaattgta taccacagac caaagcacct ttgacatttt gcaggatttg
      601 gagttttctt ctgggtcccc aggtaaagag acgaatgaga gtccttggag atcagacctg
      661 ttgatagatg aaaactgttt gctttctcct ctggcgggag aagacgattc attccttttg
      721 gaaggaaact cgaatgagga ctgcaagcct ctcattttac cggacactaa acccaaaatt
      781 aaggataatg gagatctggt tttgtcaagc cccagtaatg taacactgcc ccaagtgaaa
      841 acagaaaaag aagatttcat cgaactctgc acccctgggg taattaagca agagaaactg
      901 ggcacagttt actgtcaggc aagctttcct ggagcaaata taattggtaa taaaatgtct
      961 gccatttctg ttcatggtgt gagtacctct ggaggacaga tgtaccacta tgacatgaat
     1021 acagcatccc tttctcaaca gcaggatcag aagcctattt ttaatgtcat tccaccaatt
     1081 cccgttggtt ccgaaaattg gaataggtgc caaggatctg gagatgacaa cttgacttct
     1141 ctggggactc tgaacttccc tggtcgaaca gttttttcta atggctattc aagccccagc
     1201 atgagaccag atgtaagctc tcctccatcc agctcctcaa cagcaacaac aggaccacct
     1261 cccaaactct gcctggtgtg ctctgatgaa gcttcaggat gtcattatgg agtcttaact
     1321 tgtggaagct gtaaagtttt cttcaaaaga gcagtggaag gacagcacaa ttacctatgt
     1381 gctggaagga atgattgcat catcgataaa attcgaagaa aaaactgccc agcatgccgc
     1441 tatcgaaaat gtcttcaggc tggaatgaac ctggaagctc gaaaaacaaa gaaaaaaata
     1501 aaaggaattc agcaggccac tacaggagtc tcacaagaaa cctctgaaaa tcctggtaac
     1561 aaaacaatag ttcctgcaac gttaccacaa ctcaccccta ccctggtgtc actgttggag
     1621 gttattgaac ctgaagtgtt atatgcagga tatgatagct ctgttccaga ctcaacttgg
     1681 aggatcatga ctacgctcaa catgttagga gggcggcaag tgattgcagc agtgaaatgg
     1741 gcaaaggcaa taccaggttt caggaactta cacctggatg accaaatgac cctactgcag
     1801 tactcctgga tgtttcttat ggcatttgct ctggggtgga gatcatatag acaatcaagt
     1861 gcaaacctgc tgtgttttgc tcctgatctg attattaatg agcagagaat gactctaccc
     1921 tgcatgtacg accaatgtaa acacatgctg tatgtttcct ctgagttaca caggcttcag
     1981 gtatcttatg aagagtatct ctgtatgaaa accttactgc ttctctcttc agttcctaag
     2041 gacggtctga agagccaaga gctatttgat gaaattagaa tgacctacat caaagagcta
     2101 ggaaaagcca ttgtcaagag ggaaggaaac tccagccaga actggcagcg gttttatcaa
     2161 ctgacaaaac tcttggattc tatgcatgaa aatgttatgt ggttaaaacc agaaagcaca
     2221 tctcacacat taatctagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     2281 ctttatatat cttgtggaaa ggacgaatat caaacttcac caacaacgtc
//
