LOCUS       sequence-282633-        1277 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1457
FEATURES             Location/Qualifiers
     source          1..1277
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgtcacg catagggttg cgcatgcagc tcatgcggga gcaggcgcag
       61 caggaggagc agcgggagcg catgcagcaa caggctgtca tgcattacat gcagcagcag
      121 cagcagcagc aacagcagca gctcggaggg ccgcccaccc cggccatcaa tacccccgtc
      181 cacttccagt cgccaccacc tgtgcctggg gaggtgttga agttggatga tgtcattgac
      241 aacattatgc gtctggacga tgtccttggc tacatcaatc ctgaaatgca gatgcccaac
      301 acgctacccc tgtccagcag ccacctgaat gtgtacagca gcgaccccca ggtcacagcc
      361 tccctggtgg gcgtcaccag cagctcctgc cctgcggacc tgacccagaa gcgagagctc
      421 acagatgctg agagcagggc cctggccaag gagcggcaga agaaagacaa tcacaactta
      481 attgaaagga gacgaaggtt caacatcaat gaccgcatca aggagttggg aatgctgatc
      541 cccaaggcca atgacctgga cgtgcgctgg aacaagggca ccatcctcaa ggcctctgtg
      601 gattacatcc ggaggatgca gaaggacctg caaaagtcca gggagctgga gaaccactct
      661 cgccgcctgg agatgaccaa caagcagctc tggctccgta tccaggagct ggagatgcag
      721 gctcgagtgc acggcctccc taccacctcc ccgtccggca tgaacatggc tgagctggcc
      781 cagcaggtgg tgaagcagga gctgcctagc gaagagggcc caggggaggc cctgatgctg
      841 ggggctgagg tccctgaccc tgagccactg ccagctctgc ccccgcaagc cccgctgccc
      901 ctgcccaccc agccaccatc cccattccat cacctggact tcagccacag cctgagcttt
      961 gggggcaggg aggacgaggg tcccccgggc taccccgaac ccctggcgcc ggggcatggc
     1021 tccccattcc ccagcctgtc caagaaggat ctggacctca tgctcctgga cgactcactg
     1081 ctaccgctgg cctctgatcc acttctgtcc accatgtccc ccgaggcctc caaggccagc
     1141 agccgccgga gcagcttcag catggaggag ggcgatgtgc tgtagtaatg aactagtccg
     1201 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgacacgtaa
     1261 gctcaatgga ttacgct
//
