LOCUS       sequence-282840-        3947 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2137
FEATURES             Location/Qualifiers
     source          1..3947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtccggaa aaagaacccc cctctgagaa acgttgcaag tgaaggcgag
       61 ggccagatcc tggagcctat aggtacagaa agcaaggtat ctggaaagaa caaagaattt
      121 tctgcagatc agatgtcaga aaatacggat cagagtgatg ctgcagaact aaatcataag
      181 gaggaacata gcttgcatgt tcaagatcca tcttctagca gtaagaagga cttgaaaagc
      241 gcagttctga gtgagaaggc tggcttcaat tatgaaagcc ccagtaaggg aggaaacttt
      301 ccctcctttc cgcatgatga ggtgacagac agaaatatgt tggctttctc atctccagct
      361 gctgggggag tctgtgagcc cttgaagtct ccgcaaagag cagaggcaga tgaccctcaa
      421 gatatggcct gcaccccctc aggggactca ctggagacaa aggaagatca gaagatgtca
      481 ccaaaggcta cagaggaaac agggcaagca cagagtggtc aagccaattg tcaaggtttg
      541 agcccagttt cagtggcctc aaaaaaccca caagtgcctt cagatggggg tgtaagactg
      601 aataaatcca aaactgactt actggtgaat gacaacccag acccggcacc tctgtctcca
      661 gagcttcagg actttaaatg caatatctgt ggatatggtt actacggcaa cgaccccaca
      721 gatctgatta agcacttccg aaagtatcac ttaggactgc ataaccgcac caggcaagat
      781 gctgagctgg acagcaaaat cttggccctt cataacatgg tgcagttcag ccattccaaa
      841 gacttccaga aggtcaaccg ttctgtgttt tctggtgtgc tgcaggacat caattcttca
      901 aggcctgttt tactaaatgg gacctatgat gtgcaggtga cttcaggtgg aacattcatt
      961 ggcattggac ggaaaacacc agattgccaa gggaacacca agtatttccg ctgtaaattc
     1021 tgcaatttca cttatatggg caactcatcc accgaattag aacaacattt tcttcagact
     1081 cacccaaaca aaataaaagc ttctctcccc tcctctgagg ttgcaaaacc ttcagagaaa
     1141 aactctaaca agtccatccc tgcacttcaa tccagtgatt ctggagactt gggaaaatgg
     1201 caggacaaga taacagtcaa agcaggagat gacactcctg ttgggtactc agtgcccata
     1261 aagcccctcg attcctctag acaaaatggt acagaggcca ccagttacta ctggtgtaaa
     1321 ttttgtagtt tcagctgtga gtcatctagc tcacttaaac tgctagaaca ttatggcaag
     1381 cagcacggag cagtgcagtc aggcggcctt aatccagagt taaatgataa gctttccagg
     1441 ggctctgtca ttaatcagaa tgatctagcc aaaagttcag aaggagagac aatgaccaag
     1501 acagacaaga gctcgagtgg ggctaaaaag aaggacttct ccagcaaggg agccgaggat
     1561 aatatggtaa cgagctataa ttgtcagttc tgtgacttcc gatattccaa aagccatggc
     1621 cctgatgtaa ttgtagtggg gccacttctc cgtcattatc aacagctcca taacattcac
     1681 aagtgtacca ttaaacactg tccattctgt cccagaggac tttgcagccc agaaaagcac
     1741 cttggagaaa ttacttatcc gtttgcttgt agaaaaagta attgttccca ctgtgcactc
     1801 ttgcttctgc acttgtctcc tggggcggct ggaagctcgc gagtcaaaca tcagtgccat
     1861 cagtgttcat tcaccacccc tgacgtagat gtactcctct ttcactatga aagtgtgcat
     1921 gagtcccaag catcggatgt caaacaagaa gcaaatcacc tgcaaggatc ggatgggcag
     1981 cagtctgtca aggaaagcaa agaacactca tgtaccaaat gtgattttat tacccaagtg
     2041 gaagaagaga tttcccgaca ctacaggaga gcacacagct gctacaaatg ccgtcagtgc
     2101 agttttacag ctgccgatac tcagtcacta ctggagcact tcaacactgt tcactgccag
     2161 gaacaggaca tcactacagc caacggcgaa gaggacggtc atgccatatc caccatcaaa
     2221 gaggagccca aaattgactt cagggtctac aatctgctaa ctccagactc taaaatggga
     2281 gagccagttt ctgagagtgt ggtgaagaga gagaagctgg aagagaagga cgggctcaaa
     2341 gagaaagttt ggaccgagag ttccagtgat gaccttcgca atgtgacttg gagaggggca
     2401 gacatcctgc gggggagtcc gtcatacacc caagcaagcc tggggctgct gacgcctgtg
     2461 tctggcaccc aagagcagac aaagactcta agggatagtc ccaatgtgga ggccgcccat
     2521 ctggcgcgac ctatttatgg cttggctgtg gaaaccaagg gattcctgca gggggcgcca
     2581 gctggcggag agaagtctgg ggccctcccc cagcagtatc ctgcatcggg agaaaacaag
     2641 tccaaggatg aatcccagtc cctgttacgg aggcgtagag gctccggtgt tttttgtgcc
     2701 aattgcctga ccacaaagac ctctctctgg cgaaagaatg caaatggcgg atatgtatgc
     2761 aacgcgtgtg gcctctacca gaagcttcac tcgactccca ggcctttaaa catcattaaa
     2821 caaaacaacg gtgagcagat tattaggagg agaacaagaa agcgccttaa cccagaggca
     2881 cttcaggctg agcagctcaa caaacagcag aggggcagca atgaggagca agtcaatgga
     2941 agcccgttag agaggaggtc agaagatcat ctaactgaaa gtcaccagag agaaattcca
     3001 ctccccagcc taagtaaata cgaagcccag ggttcattga ctaaaagcca ttctgctcag
     3061 cagccagtcc tggtcagcca aactctggat attcacaaaa ggatgcaacc tttgcacatt
     3121 cagataaaaa gtcctcagga aagtactgga gatccaggaa atagttcatc cgtatctgaa
     3181 gggaaaggaa gttctgagag aggcagtcct atagaaaagt acatgagacc tgcgaaacac
     3241 ccaaattatt caccaccagg cagccctatt gaaaagtacc agtacccact ttttggactt
     3301 ccctttgtac ataatgactt ccagagtgaa gctgattggc tgcggttctg gagtaaatat
     3361 aagctctccg ttcctgggaa tccgcactac ttgagtcacg tgcctggcct accaaatcct
     3421 tgccaaaact atgtgcctta tcccaccttc aatctgcctc ctcatttttc agctgttgga
     3481 tcagacaatg acattcctct agatttggcg atcaagcatt ccagacctgg gccaactgca
     3541 aacggtgcct ccaaggagaa aacgaaggca ccaccaaatg taaaaaatga aggtcccttg
     3601 aatgtagtaa aaacagagaa agttgataga agtactcaag atgaactttc aacaaaatgt
     3661 gtgcactgtg gcattgtctt tctggatgaa gtgatgtatg ctttgcatat gagttgccat
     3721 ggtgacagtg gacctttcca gtgcagcata tgccagcatc tttgcacgga caaatatgac
     3781 ttcacaacac atatccagag gggcctgcat aggaacaatg cacaagtgga aaaaaatgga
     3841 aaacctaaag agtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     3901 tatatatctt gtggaaagga cgagggatct gtgttgctgt acagaga
//
