LOCUS       sequence-283713-        1133 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1967
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcagcc acaggaggaa gcccctgagg tccgggaaga ggaggagaaa
       61 gaggaagtgg cagaggcaga aggagcccca gagctcaatg ggggaccaca gcatgcactt
      121 ccttccagca gctacacagc tatccgtttt ggtcggatgc cggaggctga gaagaggaag
      181 ctggtggcag ggctgactgc aaacgagggg agccagtaca acccacaggt ggccgacctg
      241 aaggccttct ccaagcacat ctacaatgcc tacctgaaaa acttcaacat gaccaaaaag
      301 aaggcccgca gcatcctcac cggcaaagcc agccacacgg cgccctttgt gatccacgac
      361 atcgagacat tgtggcaggc agagaagggg ctggtgtgga agcagttggt gaatggcctg
      421 cctccctaca aggagatcag cgtgcacgtc ttctaccgct gccagtgcac cacagtggag
      481 accgtgcggg agctcactga gttcgccaag agcatcccca gcttcagcag cctcttcctc
      541 aacgaccagg ttacccttct caagtatggc gtgcacgagg ccatcttcgc catgctggcc
      601 tctatcgtca acaaggacgg gctgctggta gccaacggca gtggctttgt cacccgtgag
      661 ttcctgcgca gcctccgcaa acccttcagt gatatcattg agcctaagtt tgaatttgct
      721 gtcaagttca acgccctgga acttgatgac agtgacctgg ccctattcat tgcggccatc
      781 attctgtgtg gagaccggcc aggcctcatg aacgttccac gggtggaggc tatccaggac
      841 accatcctgc gtgccctcga attccacctg caggccaacc accctgatgc ccagtacctc
      901 ttccccaagc tgctgcagaa gatggctgac ctgcggcaac tggtcaccga gcacgcccag
      961 atgatgcagc ggatcaagaa gaccgaaacc gagacctcgc tgcaccctct gctccaggag
     1021 atctacaagg acatgtacta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1081 tggctttata tatcttgtgg aaaggacgat aacacaattc ttggaatctg tag
//
