LOCUS       sequence-283758-        4601 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0432
FEATURES             Location/Qualifiers
     source          1..4601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4080..4088
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tgatgatggc aagaaagcaa gatgtccgaa ttcccaccta caacatcagt
       61 gtggtgggat tatctgggac cgagaaggaa aagggccagt gtgggattgg aaagtcttgt
      121 ttgtgcaacc gcttcgtgcg cccgagtgct gacgagtttc acttggacca tacctccgtc
      181 ctcagcacca gtgactttgg agggcgagtg gtcaataatg accactttct ctactgggga
      241 gaagttagcc gctccctgga ggattgtgtg gaatgtaaga tgcacattgt ggagcagact
      301 gaatttattg atgatcagac ttttcaacct catcgaagca cggccctgca gccctatatc
      361 aagagagctg ctgcgaccaa gcttgcatca gctgaaaaac tcatgtactt ttgcactgac
      421 cagctggggc tggagcagga ctttgagcag aaacaaatgc cagacggaaa gctgctggtt
      481 gatggttttc ttcttggtat tgatgttagc aggggcatga ataggaactt tgatgaccag
      541 ctcaagtttg tctccaatct ctacaatcag cttgcaaaaa caaaaaagcc catagtggtg
      601 gtcctgacta agtgtgacga aggtgttgag cggtacatta gagatgcaca tacttttgcc
      661 ttaagcaaaa agaacctcca ggttgtggag acctcagcga gatccaatgt aaacgtggac
      721 ttggctttca gcaccttagt gcaactcatt gataaaagtc ggggaaagac aaaaatcatt
      781 ccttattttg aagctctcaa gcagcagagt cagcagatag ctacagcaaa agacaagtat
      841 gagtggctgg tgagtcgcat tgtgaaaaac cacaatgaga actggctgag tgtcagccga
      901 aagatgcagg cctctccaga ataccaggac tatgtctacc tggaagggac tcagaaagcc
      961 aagaagctgt ttctacagca catccaccgc ctcaagcatg agcatatcga gcgtaggaga
     1021 aagctgtacc tggcagccct gccattagct tttgaagctc ttatacctaa tctagatgaa
     1081 atagaccacc taagctgcat aaaagccaaa aagctcttag aaaccaagcc agaattcttg
     1141 aagtggtttg ttgtgcttga agagacccca tgggatgcca ccagtcacat tgacaacatg
     1201 gaaaacgaac ggattccctt tgatttaatg gataccgtcc ctgcagagca gctatacgag
     1261 gcccacttag agaagctgag gaacgaaagg aaaagagttg agatgcgaag ggcgtttaaa
     1321 gaaaacctgg agacttctcc tttcataact cccggaaagc cttgggaaga ggcccgtagt
     1381 tttattatga atgaggattt ctaccagtgg ctggaggaat ctgtatacat ggatatttat
     1441 ggcaaacacc aaaagcaaat tatagataaa gcaaaggaag aatttcagga gttgcttttg
     1501 gaatattcag aattgtttta tgaactggag ctggatgcta agcccagcaa ggagaagatg
     1561 ggtgttattc aggatgttct gggagaggaa cagcgattta aagcattaca aaagctccaa
     1621 gcagagcgtg atgcccttat tctgaaacac attcattttg tgtaccaccc aacaaaggag
     1681 acatgcccca gctgcccagc ttgtgtggac gctaagattg agcacttgat tagttctcgg
     1741 tttatccggc cgtctgaccg gaatcagaaa aattcactct ctgaccctaa cattgataga
     1801 atcaacttgg ttatattggg caaagacggc cttgcccgag agttggccaa tgagattcga
     1861 gctctttgta caaatgatga caagtatgtg atagatggta aaatgtatga gctttccctg
     1921 aggccaatag aggggaatgt caggcttcct gtgaactctt tccagacgcc aacatttcag
     1981 ccccacggct gtctctgcct ttacaattca aaggaatcgc tatcctatgt agtggaaagt
     2041 atagagaaga gtagagagtc cacgctgggc cggcgggata atcatttagt ccatctcccc
     2101 cttacattaa ttttggttaa caagagagga gacaccagtg gagagactct gcatagctta
     2161 atacagcaag gtcaacaaat tgctagcaaa cttcagtgtg tctttctcga ccctgcttct
     2221 gctggcattg gttacggacg caacattaat gaaaagcaaa tcagtcaagt tttgaaggga
     2281 ctcctggact ctaagcgtaa cttaaacctg gtcagttcta ctgctagcat caaagatttg
     2341 gctgatgttg atctgcgaat tgttatgtgt ctgatgtgtg gagatccttt tagtgcagat
     2401 gacatacttt ttcctgtcct tcagtcccaa acctgtaaat cttcccattg tggaagcaac
     2461 aactctgttt tacttgaact accaatcgga ctgcacaaga agcggattga actgtctgtt
     2521 ctttcatacc attcctcctt tagcatcaga aagagccggt tggttcatgg gtacattgtt
     2581 ttttattcag ccaaacgtaa ggcctctttg gctatgttac gtgcctttct ttgtgaagtg
     2641 caggatatta tccctattca gcttgtagca ctcactgatg gcgctgtaga tgtcctggac
     2701 aatgacttaa gtagggaaca gctaactgag ggggaggaga ttgctcaaga aattgacgga
     2761 aggttcacaa gcatcccctg tagccaaccc cagcataaac ttgagatctt tcacccattt
     2821 tttaaagatg tggtggaaaa aaagaacata atcgaggcta ctcatatgta cgataatgct
     2881 gccgaggcct gtagcaccac cgaagaggtg tttaactccc cccgggcagg atcaccgctc
     2941 tgcaactcaa acctgcagga ttcagaagaa gatatcgagc catcttacag cctgtttcga
     3001 gaagacacat cactgccttc tctgtccaaa gaccattcta agctctctat ggaactggag
     3061 ggaaatgatg ggctgtcttt cattatgagc aattttgaga gtaaactgaa caacaaagta
     3121 cctccgccag tcaaaccaaa gcctcctgtc cattttgaaa ttacaaaggg ggatctatct
     3181 tatttagacc aaggccatag ggatggacag aggaagtctg tgtcttctag cccctggctg
     3241 cctcaggatg ggtttgatcc ttctgactat gctgaaccca tggatgctgt ggtgaagcca
     3301 aggaatgaag aagaaaacat atactccgtg ccccatgaca gcacccaagg caaaatcatc
     3361 accattcgga atatcaacaa agcccagtcc aacggcagcg ggaatggttc tgacagtgaa
     3421 atggacacca gctctctaga gcgagggcgc aaggtttcca tcgtgagcaa gccagtgctg
     3481 tacaggacga gatgcacccg gctggggcgg tttgctagtt accggaccag cttcagcgtg
     3541 gggagtgatg atgagctggg gcccatccgg aagaaagagg aggatcaggc atcccagggt
     3601 tataaagggg acaatgctgt cattccatac gaaacagacg aagacccgcg gaggaggaat
     3661 attcttcgca gcctaaggag gaacactaag aaaccaaagc ccaaaccccg gccatccatc
     3721 acaaaggcaa cctgggagag taactatttt ggggtgccct taacaactgt cgtgactcca
     3781 gagaagccga tccccatttt tattgaaaga tgtattgagt acattgaagc cacaggactg
     3841 agcacggaag gcatctaccg ggtcagcggg aacaagtctg agatggagag tctgcagaga
     3901 cagtttgatc aagaccacaa cctggacctg gcagagaaag actttacggt gaataccgtg
     3961 gctggtgcca tgaagagctt tttctcagaa ctgcctgacc ccctggtccc gtataacatg
     4021 cagatcgact tggtggaagc acacaaaatc aacgaccggg agcagaagtt gcatgccctt
     4081 aaggaggtat taaagaaatt tccaaaggaa aaccacgaag tcttcaagta tgtcatctct
     4141 cacctaaaca aggtcagcca caacaacaag gtgaatctca tgaccagcga gaacctctcc
     4201 atctgcttct ggcccacctt gatgagacct gatttcagca ctatggacgc cctcacagcc
     4261 acgcgcacct accagacaat cattgaactc tttatccagc agtgcccctt cttcttctac
     4321 aatcggccca tcaccgagcc ccccggcgcc aggcccagct ccccctctgc cgtggcttcc
     4381 accgtcccct tcctcacttc cacgcctgtc acaagtcagc cgtcgccccc acagtcgcct
     4441 ccacccaccc cccagtcccc aatgcagcca ctgcttccct cccagcttca agccgaacac
     4501 acgctgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     4561 tcttgtggaa aggacgacta agcattgttt actgaatctg g
//
