LOCUS       sequence-283881-        2210 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1065
FEATURES             Location/Qualifiers
     source          1..2210
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1519..1528
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgtcacctcg aaccagcctc gccgaggaca gctgcctggg ccgccactcg
       61 cccgtgcccc gtccggcctc ccgctcctca tcgcctggtg ccaagcggag gcattcgtgc
      121 gccgaggcct tggttgccct gccgcccgga gcctcacccc agcgctcccg gagcccctcg
      181 ccgcagccct catctcacgt ggcaccccag gaccacggct ccccggctgg gtacccccct
      241 gtggctggct ctgccgtgat catggatgcc ctgaacagcc tcgccacgga ctcgccttgt
      301 gggatccccc ccaagatgtg gaagaccagc cctgacccct cgccggtgtc tgccgcccca
      361 tccaaggccg gcctgcctcg ccacatctac ccggccgtgg agttcctggg gccctgcgag
      421 cagggcgaga ggagaaactc ggctccagaa tccatcctgc tggttccgcc cacttggccc
      481 aagccgctgg tgcctgccat tcccatctgc agcatcccag tgactgcatc cctccctcca
      541 cttgagtggc cgctgtccag tcagtcaggc tcttacgagc tgcggatcga ggtgcagccc
      601 aagccacatc accgggccca ctatgagaca gaaggcagcc gaggggctgt caaagctcca
      661 actggaggcc accctgtggt tcagctccat ggctacatgg aaaacaagcc tctgggactt
      721 cagatcttca ttgggacagc tgatgagcgg atccttaagc cgcacgcctt ctaccaggtg
      781 caccgaatca cggggaaaac tgtcaccacc accagctatg agaagatagt gggcaacacc
      841 aaagtcctgg agataccctt ggagcccaaa aacaacatga gggcaaccat cgactgtgcg
      901 gggatcttga agcttagaaa cgccgacatt gagctgcgga aaggcgagac ggacattgga
      961 agaaagaaca cgcgggtgag actggttttc cgagttcaca tcccagagtc cagtggcaga
     1021 atcgtctctt tacagactgc atctaacccc atcgagtgct cccagcgatc tgctcacgag
     1081 ctgcccatgg ttgaaagaca agacacagac agctgcctgg tctatggcgg ccagcaaatg
     1141 atcctcacgg ggcagaactt tacatccgag tccaaagttg tgtttactga gaagaccaca
     1201 gatggacagc aaatttggga gatggaagcc acggtggata aggacaagag ccagcccaac
     1261 atgctttttg ttgagatccc tgaatatcgg aacaagcata tccgcacacc tgtaaaagtg
     1321 aacttctacg tcatcaatgg gaagagaaaa cgaagtcagc ctcagcactt tacctaccac
     1381 ccagtcccag ccatcaagac ggagcccacg gatgaatatg accccactct gatctgcagc
     1441 cccacccatg gaggcctggg gagccagcct tactaccccc agcacccgat ggtggccgag
     1501 tccccctcct gcctcgtggc caccatggct ccctgccagc agttccgcac ggggctctca
     1561 tcccctgacg cccgctacca gcaacagaac ccagcggccg tactctacca gcggagcaag
     1621 agcctgagcc ccagcctgct gggctatcag cagccggccc tcatggccgc cccgctgtcc
     1681 cttgcggacg ctcaccgctc tgtgctggtg cacgccggct cccagggcca gagctcagcc
     1741 ctgctccacc cctctccgac caaccagcag gcctcgcctg tgatccacta ctcacccacc
     1801 aaccagcagc tgcgctgcgg aagccaccag gagttccagc acatcatgta ctgcgagaat
     1861 ttcgcaccag gcaccaccag acctggcccg cccccggtca gtcaaggtca gaggctgagc
     1921 ccgggttcct accccacagt cattcagcag cagaatgcca cgagccaaag agccgccaaa
     1981 aacggacccc cggtcagtga ccaaaaggaa gtattacctg cgggggtgac cattaaacag
     2041 gagcagaact tggaccagac ctacttggat gatgagctga tagacacaca ccttagctgg
     2101 atacaaaaca tattatagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     2161 ctttatatat cttgtggaaa ggacgaagaa attatcaatt gttatatacc
//
