LOCUS       sequence-283899-        2984 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1100
FEATURES             Location/Qualifiers
     source          1..2984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccttgct gggtaagcgc tgtgacgtcc ccaccaacgg ctgcggaccc
       61 gaccgctgga actccgcgtt cacccgcaaa gacgagatca tcaccagcct cgtgtctgcc
      121 ttagactcca tgtgctcagc gctgtccaaa ctgaacgccg aggtggcctg tgtcgccgtg
      181 cacgatgaga gcgcctttgt ggtgggcaca gagaagggga gaatgttcct gaatgcccgg
      241 aaggagctac agtcagactt cctcaggttc tgccgagggc ccccgtggaa ggatccggag
      301 gcagagcacc ccaagaaggt gcagcggggc gagggtggag gccgtagcct ccctcggtcc
      361 tccctggaac atggctcaga tgtgtacctt ctgcggaaga tggtagagga ggtgtttgat
      421 gttctttata gcgaggccct gggaagggcc agtgtggtgc cactgcccta tgagaggctg
      481 ctcagggagc cagggctgct ggccgtgcag gggctgcccg aaggcctggc cttccgaagg
      541 ccagccgagt atgaccccaa ggccctcatg gccatcctgg aacacagcca ccgcatccgc
      601 ttcaagctca agaggccact tgaggatggc gggcgggact cgaaggccct ggtggagctg
      661 aacggtgtct ccctgattcc caaggggtca cgggactgtg gcctgcatgg ccaggccccc
      721 aaggtgccac cccaggacct gcccccaacc gccacctcct cctccatggc cagcttcctg
      781 tacagcacgg cgctccccaa ccacgccatc cgagagctca agcaggaagc accttcctgc
      841 ccccttgccc ccagcgacct gggcctgagt cggcccatgc cagagcccaa ggccaccggt
      901 gcccaagact tctccgactg ttgtggacag aagcccactg ggcctggtgg gcctctcatc
      961 cagaacgtcc atgcctccaa gcgcattctc ttctccatcg tccatgacaa gtcagagaag
     1021 tgggacgcct tcataaagga aaccgaggac atcaacacgc tccgggagtg tgtgcagatc
     1081 ctgtttaaca gcagatatgc ggaagccctg ggcctggacc acatggtccc cgtgccctac
     1141 cggaagattg cctgtgaccc ggaggctgtg gagatcgtgg gcatcccgga caagatcccc
     1201 ttcaagcgcc cctgcactta tggagtcccc aagctgaagc ggatcctgga ggagcgccat
     1261 agtatccact tcatcattaa gaggatgttt gatgagcgaa ttttcacagg gaacaagttt
     1321 accaaagaca ccacgaagct ggagccagcc agcccgccag aggacacctc tgcagaggtc
     1381 tctagggcca ccgtccttga ccttgctggg aatgctcggt cagacaaggg cagcatgtct
     1441 gaagactgtg ggccaggaac ctccggggag ctgggcgggc tgaggccgat caaaattgag
     1501 ccagaggatc tggacatcat tcaggtcacc gtcccagacc cctcgccaac ctctgaggaa
     1561 atgacagact cgatgcctgg gcacctgcca tcggaggatt ctggttatgg gatggagatg
     1621 ctgacagaca aaggtctgag tgaggacgcg cggcccgagg agaggcccgt ggaggacagc
     1681 cacggtgacg tgatccggcc cctgcggaag caggtggagc tgctcttcaa cacacgatac
     1741 gccaaggcca ttggcatctc ggagcccgtc aaggtgccgt actccaagtt tctgatgcac
     1801 ccggaggagc tgtttgtggt gggactgcct gaaggcatct ccctccgcag gcccaactgc
     1861 ttcgggatcg ccaagctccg gaagattctg gaggccagca acagcatcca gtttgtcatc
     1921 aagaggcccg agctgctcac tgagggagtc aaagagccca tcatggatag tcaagagagg
     1981 gattccgggg accctctggt ggacgagagc ctgaagagac agggctttca agaaaattat
     2041 gacgcgaggc tctcacggat cgacatcgcc aacacactaa gggagcaggt ccaggacctt
     2101 ttcaataaga aatacgggga agccttgggc atcaagtacc cggtccaggt cccctacaag
     2161 cggatcaaga gtaaccccgg ctccgtgatc atcgaggggc tgcccccagg aatcccgttc
     2221 cgaaagccct gtaccttcgg ctcccagaac ctggagagga ttcttgctgt ggctgacaag
     2281 atcaagttca cagtcaccag gcctttccaa ggactcatcc caaagcctga tgaagatgac
     2341 gccaacagac tcggggagaa ggtgatcctg cgggagcagg tgaaggaact cttcaacgag
     2401 aaatacggtg aggccctggg cctgaaccgg ccggtgctgg tcccttataa actaatccgg
     2461 gacagcccag acgccgtgga ggtcacgggt ctgcctgatg acatcccctt ccggaacccc
     2521 aacacgtacg acatccaccg gctggagaag atcctgaagg cccgagagca tgtccgcatg
     2581 gtcatcatta accagctcca accctttgca gaaatctgca atgatgccaa ggtgccagcc
     2641 aaagacagca gcattcccaa gcgcaagaga aagcgggtct cggaaggaaa ttccgtctcc
     2701 tcttcctcct cgtcttcctc ttcctcgtcc tctaacccgg attcagtggc atcggccaac
     2761 cagatctcac tcgtggtaaa gttgcaccga tttggactcc ggcactcatc tctgtggccc
     2821 tcacccctct gtctggcagg gccgtctact ctgggatgtg ggcccagggg acggggaggc
     2881 actgggcttt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     2941 atatcttgtg gaaaggacga ctgcctgact acaagagatg cttg
//
