LOCUS       sequence-283974-        3326 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1308
FEATURES             Location/Qualifiers
     source          1..3326
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             750..758
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tggtgtattt tggaagctct caggatgagg aggaagtcga ggaggaagat
       61 gatgagacag aagacgtcaa aacagccacc aacaatgctt catcttcatg ccagtcgacc
      121 cccaggaaag gaaaaaccca caaacatgtt cacaacgggc atgttttcaa tggttccagc
      181 aggtcaacac gggagaagga acctgttcaa aaacacaaaa gcaaagaggc cactcccgca
      241 aaggagaagc acagcgatca ccgggctgac agccgccggg agcaggcttc agctaaccac
      301 cccgcagcgg ccccctccac gggttcctcg gccaaggggc ttgctgccac ccatcaccac
      361 ccccctctgc atcggtcggc tcaggactta cggaaacagg tttctaaggt aaacggagtc
      421 actcgaatgt catctctggg tgcaggtgta accagtgcca aaaagatgcg cgaggtcaga
      481 ccttcaccat ccaaaactgt gaagtacact gccacggtga cgaagggggc tgtcacatac
      541 accaaagcca agagagaact ggtcaaggac accaaaccca atcaccacaa gcccagttcc
      601 gctgtcaacc acacaatctc agggaaaact gaaagtagca atgcaaaaac ccgcaaacag
      661 gtgctatccc tcgggggggc gtccaagtcc actgggcccg ccgtcaatgg cctcaaggtc
      721 agtggcaggt tgaacccaaa gtcatgcact aaggaggtgg gggggcggca gctgcgggag
      781 ggcctgcagc tgcgggaggg gctgcggaac tccaagagga gactggaaga ggcacaccag
      841 gcggagaagc cgcagtcgcc ccccaagaag atgaaagggg cggctggccc cgccgaaggc
      901 cctggcaaga aggccccggc cgagagaggt ctgctgaacg gacacgtgaa gaaggaagtg
      961 ccggagcgca gtctggagag gaatcggccg aagcgggcca cggccgggaa gagcacgcca
     1021 ggcagacaag cacatggcaa ggcggacagc gcctcctgtg aaaatcgttc tacctcgcaa
     1081 ccggagtccg tgcacaagcc gcaggactcg ggcaaggccg agaagggcgg cggcaaggcc
     1141 gggtgggcgg ccatggacga gatccccgtc ctcaggccct ccgccaagga gttccacgat
     1201 ccgctcatct acatcgagtc ggtccgcgct caggtggaga agttcgggat gtgcagggtg
     1261 atcccccctc cggactggcg gcccgagtgc aagctcaacg atgagatgcg gtttgtcacg
     1321 cagattcagc acatccacaa gctgggccgg cgctggggcc ccaacgtgca gcggctggcc
     1381 tgcatcaaga agcacctcaa atctcagggc atcaccatgg acgagctccc gctcataggg
     1441 ggctgtgagc tcgacctggc ctgctttttc cggctgatta atgagatggg cggcatgcag
     1501 caagtgactg acctcaaaaa atggaacaaa ctagcagaca tgctgcgcat ccccagaact
     1561 gcccaggacc ggctggccaa gctgcaggag gcctactgcc agtacctact ctcctacgac
     1621 tccctgtccc cagaggagca ccggcggctg gagaaggagg tgctgatgga gaaggagatc
     1681 ctggagaagc gcaaggggcc gctggaaggc cacacagaga acgaccacca caagttccac
     1741 cctctgcccc gcttcgagcc caagaatggg ctcatccacg gcgtggcccc caggaacggc
     1801 ttccgcagca agctcaagga ggtgggccag gcccagttga agactggccg gcggcgactc
     1861 ttcgctcagg aaaaagaagt ggtcaaggaa gaggaggagg acaaaggcgt cctcaatgac
     1921 ttccacaagt gcatctataa gggaaggtct gtttctctaa caacttttta tcgaacagcg
     1981 aggaatatca tgagcatgtg tttcagcaag gagcctgccc cagccgaaat cgagcaagag
     2041 tactggaggc tagtggaaga gaaggactgc cacgtggcag tgcactgcgg caaggtggac
     2101 accaacactc acggcagtgg attcccagta ggaaaatcag aacccttttc gaggcatgga
     2161 tggaacctca ccgtcctccc caataacaca gggtccatcc tgcgtcacct cggtgctgtg
     2221 cctggagtga ctattccctg gctaaatatt ggcatggtct tttctacctc atgctggtct
     2281 cgagaccaaa atcaccttcc atacattgac tacttacaca ctggtgctga ctgcatttgg
     2341 tattgcattc ctgctgagga ggagaacaag ctggaagatg tggtccacac cctgctgcaa
     2401 gccaatggca ccccagggct gcagatgctg gaaagcaacg tcatgatctc cccggaggtg
     2461 ctgtgcaaag aggggatcaa ggtgcacagg accgtgcagc agagtggcca gtttgtcgtc
     2521 tgcttcccgg gatcctttgt gtccaaagtg tgctgtgggt acagcgtgtc tgaaaccgtg
     2581 cactttgcta ccacccagtg gacaagtatg ggctttgaga ccgccaagga aatgaagcgt
     2641 cgccatatag ctaagccatt ctccatggag aagttactct accagattgc acaagcagaa
     2701 gcaaaaaaag aaaacggtcc cactctcagt accatctcag ccctcctgga tgagctcagg
     2761 gatacagagc tgcggcagcg caggcagctg ttcgaggctg gcctccactc ctccgcacgc
     2821 tatggcagcc acgatggcag cagcacggtg gcggacggga agaaaaagcc tcgaaagtgg
     2881 ctgcagttgg agacgtcaga gaggaggtgt cagatctgcc agcacctgtg ctacctgtcc
     2941 atggtggtac aagagaacga aaacgtcgtg ttctgtctgg agtgtgctct gcgccacgtg
     3001 gagaaacaga agtcctgccg agggctgaag ttgatgtacc gctacgatga ggaacagatt
     3061 atcagtctgg tcaatcagat ctgcggcaaa gtgtctggta aaaacggcag cattgagaac
     3121 tgtctcagta aacccacacc aaaaagaggt ccccgcaaga gagcgacagt ggacgtgccc
     3181 ccctcccgtc tgtcagcctc cagttcatcc aaaagtgctt cgagctcatc atagtaatga
     3241 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     3301 gaagggccgc ttccacacgg gttaaa
//
