LOCUS       sequence-28399-e         867 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP26
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..26
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     36..60
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      133..142
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             142..165
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             178..204
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    220..244
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
       61 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      121 ctccggggat ctgccaccat ggactacaag gatgacgatg acaagctcga tggaggatac
      181 ccatacgatg ttccagatta cgctgctcga ggttatcaaa caagtttgta caaaaaagtt
      241 ggcatggctg ccctattcct acgtggtttt gtccaaatag ggaactgcaa gactgggata
      301 tctaagtcaa aagaagcatt cattgaagca gtggaaagaa agaagaaaga tagactggtg
      361 ctgtatttca aaagtggaaa atatagcact tttcggctaa gtgataatat tcaaaatgta
      421 gtccttaaat cctatagagg aaaccaaaat cacctgcatt taactttaca aaataataat
      481 ggcttgttta ttgaaggatt atcctccaca gatgctgaac aattgaagat attcttggac
      541 agagttcatc aaaacgaggt tcagccacct gtgagacctg gtaagggtgg gagtgtcttt
      601 tctagcacaa cacagaagga aatcaacaaa acttcattcc acaaagttga tgagaaatca
      661 agtagcaaat cttttgagat agcaaaagga agtgggacag gtgtccttca gaggatgcct
      721 ttgcttacat caaaattgac acttacttgc ggagagttat cagaaaatca gcacaagaag
      781 aggaaaagaa tgctctcatc tagctcanag atgaatgaag aattcttgaa agaaaataat
      841 tctgtagaat acaagaaatc caaggca
//
