LOCUS       sequence-284036-       10852 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10852)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1708
FEATURES             Location/Qualifiers
     source          1..10852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6709..7297
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6762..6782)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7180..7191)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7369..7602
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7680..7801
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7717..7736)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7771..7790
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7841..7976
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7996..8015)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7997..8015)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8025..8042)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8025..8041)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8034..8056)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8183..8638
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8270..8289)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8480..8501
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8664..8768
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8769..8837,8838..9629)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8987..9006)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9800..10388
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10289..10308
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10542..10559
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10676..10697
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10712..10717,10718..10735,10736..10742)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10750..10766
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10755..10777
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10774..10790
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10774..10790
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10809..10829
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10811..10829
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggac agcgaggcat tccagagcgc
     4141 gcgggacttt ctggacatga acttccagtc gctggccatg aaacacatgg atctgaagca
     4201 gatggagctg gacacggcgg cggccaaggt ggatgaactg accaagcagc tggagtcgct
     4261 gtggtcagac tctcccgcgc ctcctggccc gcaggccgga cccccttcta ggccgccccg
     4321 gtacagctcc agctcgatcc ctgagccctt cggcagccga gggtcccccc ggaaggcggc
     4381 caccgacggc gcagacaccc cgttcggacg atcagagagt gccccaaccc tacaccccta
     4441 cagcccgctg tcccccaagg gacggccgtc gtcgccgcgc accccgctct acctgcagcc
     4501 ggacgcctac ggcagcctgg accgcgcgac ctcgccccgg ccccgcgcct tcgatggcgc
     4561 aggcagctcc ctcggccgtg cgccctcccc gcggcccggg ccaggcccgc tccgccagca
     4621 gggtcccccc acgcctttcg acttcctggg ccgcgcaggc tccccccgcg gcagccccct
     4681 ggcggagggg ccccaggcct tcttccccga gcgtgggccg tcaccgcgcc cccctgccac
     4741 agcctacgac gcgccagcgt ccgccttcgg gagctccctg ctaggctccg gcggcagcgc
     4801 attcgccccg cctctgcgcg cgcaagacga cctgacgctg cgccggcggc ctccgaaagc
     4861 ctggaacgag tctgacctgg acgtggcgta cgagaagaag ccttcgcaga cagcgagcta
     4921 tgaacgcctg gacgtcttcg caaggcctgc ctcgccgagc ctgcagctgt tgccttggag
     4981 ggagagcagc ctggatggac tggggggcac cggcaaggac aacctcacta gcgccaccct
     5041 gccgcgcaat tacaaggtct ctcctctggc cagcgaccgg cgttcagacg cgggcagcta
     5101 ccggcgctcg ctgggctccg cggggccgtc gggcactttg cctcgcagct ggcagcccgt
     5161 cagccgcatc cccatgcccc cctccagccc ccagccccgc ggggccccgc gccagcgtcc
     5221 catccccctc agcatgatct tcaagctgca gaacgccttc tgggagcacg gggccagccg
     5281 cgccatgctc cctgggtccc ccctcttcac ccgagcaccc ccgcctaagc tgcagcccca
     5341 accacaacca cagccccagc cacaatcaca accacagccc cagctgcccc cacagcccca
     5401 gacccaaccc caaaccccta ccccagcccc ccagcatccc caacagacat ggccccctgt
     5461 gaacgaagga ccccccaaac cccccaccga gctggagcct gagccggaga tagaggggct
     5521 gctgacacca gtgctggagg ctggcgatgt ggatgaaggc cctgtagcaa ggcctctcag
     5581 ccccacgagg ctgcagccag cactgccacc ggaggcacag tcggtgcccg agctggagga
     5641 ggtggcacgg gtgttggcgg aaattccccg gcccctcaaa cgcaggggct ccatggagca
     5701 ggcccctgct gtggccctgc cccctaccca caagaaacag taccagcaga tcatcagccg
     5761 cctcttccat cgtcatgggg ggccagggcc cggggggccg gagccagagc tgtcccccat
     5821 cactgaggga tctgaggcca gggcagggcc ccctgctcct gccccaccag ctcccattcc
     5881 acccccggcc ccgtcccaga gcagcccacc agagcagccg cagagcatgg agatgcgctc
     5941 tgtgctgcgg aaggcgggct ccccgcgcaa ggcccgccgc gcgcgcctca accctctggt
     6001 gctcctcctg gacgcggcgc tgaccgggga gctggaggtg gtgcagcagg cggtgaagga
     6061 gatgaacgac ccgagccagc ccaacgagga gggcatcact gccttgcaca acgccatctg
     6121 cggcgccaac tactctatcg tggatttcct catcaccgcg ggtgccaatg tcaactcccc
     6181 cgacagccac ggctggacac ccttgcactg cgcggcgtcg tgcaacgaca cagtcatctg
     6241 catggcgctg gtgcagcacg gcgctgcaat cttcgccacc acgctcagcg acggcgccac
     6301 cgccttcgag aagtgcgacc cttaccgcga gggttatgct gactgcgcca cctacctggc
     6361 agacgtcgag cagagtatgg ggctgatgaa cagcggggca gtgtacgctc tctgggacta
     6421 cagcgccgag ttcggggacg agctgtcctt ccgcgagggc gagtcggtca ccgtgctgcg
     6481 gagggacggg ccggaggaga ccgactggtg gtgggccgcg ctgcacggcc aggagggcta
     6541 cgtgccgcgg aactacttcg ggctgttccc cagggtgaag cctcaaagga gtaaagtcta
     6601 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     6661 aaaggacgaa gaaacatgct cggtgctacg gttacgcgtt aagtcgacaa tcaacctctg
     6721 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6781 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6841 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6901 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6961 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7021 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7081 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7141 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7201 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7261 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     7321 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     7381 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     7441 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7501 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7561 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     7621 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     7681 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7741 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7801 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     7861 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7921 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7981 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     8041 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8101 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     8161 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     8221 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     8281 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     8341 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     8401 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     8461 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     8521 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     8581 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     8641 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8701 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8761 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8821 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8881 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8941 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9001 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9061 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9121 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9181 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9241 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9301 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9361 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9421 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9481 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9541 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9601 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9661 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9721 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9781 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9841 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9901 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     9961 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10021 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10081 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10141 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10201 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10261 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10321 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10381 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10441 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10501 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10561 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    10621 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    10681 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    10741 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    10801 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gc
//
