LOCUS       sequence-284427-        2105 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1251
FEATURES             Location/Qualifiers
     source          1..2105
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggagctgc cggccttcag ggcgcgcctg gtggcgccga gcatcctggg
       61 aggtgtggtc cgggcgctgt gccgaggatt cggggtagtg tagtcctggc gccccgctgg
      121 aggagctccc cagctggtgg ctggagcgac cccttgttct ttggtggcgc tggaatttca
      181 ttttctaaac cagaactcat cacccagctg gagcaaggga aagagacctg gagagaggaa
      241 aaaaaatgtt caccggcaac ctgtccagat ccagagccag agctctacct cgatcctttc
      301 tgccctccgg gtttctccag tcagaaattc cccatgcagc atgtgctgtg taatcatccc
      361 ccctggatct tcacatgctt gtgtgcagaa ggtaacatcc agcctgggga tccgggccca
      421 ggggaccagg agaagcagca acaagcctct gaggggagac cctggagtga tcaagcagaa
      481 ggtcctgagg gagaaggtgc catgcctttg tttggaagaa ccaagaaaag gactctggga
      541 gcgttctcca ggccacccca gaggcagcca gtcagctctc ggaacggcct cagaggggtg
      601 gagttagaag ccagcccagc tcagtcaggg aaccctgagg aaacagacaa attgttgaag
      661 aggatagaag tcttaggatt tggaacagtc aactgtggag agtgtggact gagcttcagc
      721 aagatgacaa acctgctcag tcaccagcgg atacactcag gggagaagcc ctacgtgtgt
      781 ggggtatgtg agaagggctt cagcctaaag aagagcctcg ccagacacca gaaggcacac
      841 tcgggggaga agccaattgt gtgcagggag tgtggacgag gctttaaccg gaagtcaacg
      901 ctaatcatac acgaacggac acactccggt gagaaacctt acatgtgcag tgagtgtggg
      961 cgaggcttca gccagaagtc aaacctcatc atacaccaga ggacacactc aggggaaaag
     1021 ccttacgtgt gccgggaatg tggcaaaggc ttcagccaga agtcagctgt cgtgagacac
     1081 cagaggacac acttggagga gaagaccatc gtgtgcagtg actgtggcct gggcttcagc
     1141 gacaggtcaa acctcatctc ccaccagagg acgcactctg gggagaagcc ctacgcctgc
     1201 aaggagtgtg ggcgatgctt caggcagagg accacccttg tcaaccacca gaggacacac
     1261 tcaaaggaga agccctatgt gtgcggggtg tgtgggcaca gcttcagcca gaattcaacc
     1321 ctcatctctc acaggcggac acacactggg gagaagccgt atgtttgtgg ggtgtgtggg
     1381 cgaggcttta gtctcaagtc acacctcaac agacaccaga acatacactc aggagagaag
     1441 cccattgtgt gcaaggactg tggccggggc ttcagccagc aatccaacct catcagacac
     1501 cagaggacgc actcaggcga gaagcccatg gtgtgtgggg agtgcgggcg aggcttcagc
     1561 cagaagtcaa accttgttgc acaccagagg acgcactcag gggagaggcc gtatgtgtgc
     1621 cgagagtgcg ggcgaggctt tagccaccag gccggtctca tcaggcacaa gcggaagcac
     1681 tcgagggaga agccctacat gtgcaggcag tgtggactgg gctttggcaa taagtcagct
     1741 ctaattacac acaagcgggc tcactcggaa gagaagcctt gtgtgtgcag agagtgtggc
     1801 caaggctttc tccaaaagtc acacctcacc ttacatcaaa tgacacatac gggggagaag
     1861 ccatatgtgt gcaagacgtg tgggcggggc ttcagcctca agtctcacct cagcagacac
     1921 aggaagacca cgtctgtcca ccacagactg ccagtgcagc ccgaccctga gccgtgtgca
     1981 gggcaacctt cggattcctt atactctctc tagtaatgaa ctagtccgta acttgaaagt
     2041 atttcgattt cttggcttta tatatcttgt ggaaaggacg attggcagat cggtgcgtta
     2101 cggcg
//
