LOCUS       sequence-284589-        2138 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2739
FEATURES             Location/Qualifiers
     source          1..2138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcttcc tcagggacag ttgacattca aggatgtggc catagaattc
       61 tctcaggagg agtggacatg cctggaccct gctcagaaga ctttgtacag ggacgtcatg
      121 ttggagaatt ataggaacct ggtctccctg gatatctctt gtaaatgtgt aaacacggat
      181 ttgccaccaa aggggaagaa caatatggga gaagcgttct acacggtgaa gttggagaga
      241 cttgaaagct gtgacactgt aggcttgtcc ttccaggaag ttcagaaaaa tacatacgac
      301 tttgagtgtc agtggaaaga tgatgaagga aattataaaa cagtacttat gttgcaaaaa
      361 gaaaatctcc ctggtagaag agctcaacgt gatagaaggg ctgcaggaaa caggcatatt
      421 gaaaatcagc ttggagtaag ctttcagtca catctccctg aactgcagca atttcaacat
      481 gaagggaaaa tttatgaata caatcaagtt gagaagtctc ctaataatcg aggaaaacat
      541 tataaatgtg atgaatgtgg caaggtcttc agtcaaaact cacggctaac aagtcataag
      601 agaattcata ctggagagaa gccttaccag tgtaataagt gtggcaaagc ctttactgtt
      661 cgttcaaacc taacaatcca tcaggtcatc catactggag aaaaacctta caaatgtaat
      721 gaatgtggaa aggtcttcag tcaaccttca aaccttgcag gtcatcagag aattcatact
      781 ggagagaaac cttacaagtg taatgagtgt ggcaaagcct ttagagcaca ttcaaaacta
      841 actacacatc aggtcatcca tactggagaa aaaccttaca aatgtaagga atgtggcaag
      901 tgcttcactc aaaattcaca ccttgcaagt catcgaagaa ttcatactgg ggagaagcct
      961 tacaagtgta atgagtgtgg caaagccttt agtgttcgct caagcctgac tacccatcag
     1021 acaatccaca ctggagaaaa accttacaaa tgtaatgaat gtggcaaggt cttcaggcac
     1081 aattcatacc ttgcaaagca tcggcgaatt catactggtg agaaacctta caagtgtaat
     1141 gagtgtggga aagccttcag tatgcattca aacttaacta agcatcagat catccatacc
     1201 ggagaaaagc ctttcaaatg taatgaatgc gtcaaggttt tcactcagta ttcacactta
     1261 gcaaatcatc gaagaattca tactggagag aaaccttaca ggtgtgatga gtgtggcaaa
     1321 gcctttagtg tgcgatcaag cctaactacc catcaggcaa ttcatactgg agaaaagcct
     1381 tacaaatgta atgactgcgg taaggtcttc acacaaaatt cacaccttgc aagtcatcgg
     1441 ggaattcatt ctggagagaa accttacaag tgtgatgaat gtggtaaagc cttcagtcaa
     1501 acatcacaac ttgcaaggca ttggagagtt catactggag aaaaacctta caagtgtaat
     1561 gagtgtggca aagcctttag tgttcattca agcctaacta tacatcagac aatacatact
     1621 ggacaaaaac catacaaatg taatgattgc ggcaaggtct tcagacacaa ttcgtacctt
     1681 gcaattcatc agagaattca cactggtgag aaaccttata agtgtaatga gtgcggcaaa
     1741 gcatttagtg tacattcaaa cctagctacc catcaggtca tccatactgg agaaaaacct
     1801 tacaaatgta atgaatgtgg caaggtcttc actcaaaatt cacatcttgc aaatcataga
     1861 agaattcaca ctggagaaaa accttatagg tgtaatgagt gtgggaaagc cttcagtgtt
     1921 cgttcaaccc taactaccca tatggcagtc catactggag acaaacctta caaatgtaac
     1981 caatgtggca aggtctttac tcaaaattca aaccttgcaa aacatcgaag aattcatagt
     2041 ggatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2101 tgtggaaagg acgagctcga ggtccaaggc accatagg
//
