LOCUS       sequence-284781-        1649 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2723
FEATURES             Location/Qualifiers
     source          1..1649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagagctt ggggctgcac acggtgaccc ttagtgatgg gacaacagcc
       61 tacgtccagc aagctgtcaa aggagagaag cttcttgaag ggcaggtgat ccagctcgag
      121 gatgggacca ccgcatacat tcaccaggtg acggtacaga aagaagctct ctcctttgag
      181 gatggtcagc ctgtgcagct ggaagatggc agcatggctt acatacaccg cacacccaga
      241 gaaggctatg accccagcac cctggaagcc gtccaactgg aagatggctc cactgcctac
      301 attcaccacc ctgtggctgt gccatcggag agcaccatcc tggccgtaca gacagaggtg
      361 ggcttggagg acctggcagc agaggatgat gagggcttca gtgcagacgc agtggtggcc
      421 ctggagcagt atgccagcaa ggttcttcat gacagccaga ttccccgtaa tggaaaaggg
      481 cagcaagttg gagacagagc attccgctgt ggctacaagg gctgtgggcg tctctacacc
      541 accgctcatc acttaaaggt gcatgaacga gctcatacag gtgaccgtcc atacagatgt
      601 gacttcccca gctgtggaaa ggcctttgcc acaggctatg gactgaagag ccacgttcgt
      661 acccacactg gtgagaaacc atacaagtgc ccagaggagc tgtgcagcaa ggccttcaag
      721 acctcaggag acctgcagaa gcatgtccgt acccacactg gtgaacgccc gttccagtgc
      781 ccttttgagg gctgtggccg ctccttcacc acatctaaca tccgcaaggt acatgtgcgc
      841 acccacacag gcgagaggcc ctacacctgc ccggagcccc actgtggccg cggcttcacc
      901 agcgccacca actataagaa tcacgtgcgc atccacacag gggagaagcc atacgtttgc
      961 acggtgccag gctgcgggaa acgcttcacc gagtactcga gcttgtataa gcaccacgtg
     1021 gtgcacacac actgcaagcc ctacacctgc agcacctgcg gcaagaccta ccggcagacc
     1081 tccaccttgg ccatgcacaa gcgcagtgcc cacggcgagc tggaggccac ggaggagagc
     1141 gagcaggccc tctatgagca gcagcaactt gaggccgcct ctgcagccga ggagagtccg
     1201 ccacccaaac gaccccggat agcttacctt tcggaggtga aggaagagag agatgacatc
     1261 ccagcccagg tggcgatggt gactgaagaa gatggggccc cccaggtggc tctgatcact
     1321 caggatggtg cccagcaggt cacaatcatt acctctgggg ctgtggtggc tgaggactca
     1381 agtgtagcat ctcttcgtca tcaacaggtg gcactgttgg ccacagccaa cggaacgcac
     1441 attgcagtgc agctggagga acagcagacc ttagaggagg ccatcaatgt ggccactgcg
     1501 gccatgcagc aaggggctgt gaccctggag acaacagtgt cggagagtgg ctgctagtaa
     1561 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1621 gacgacttca ccacctttcc cggattcaa
//
