LOCUS       sequence-284817-        4784 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2775
FEATURES             Location/Qualifiers
     source          1..4784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    1009..1020
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 accgccacca tggagccggg gtccgacgat ttcctaccgc caccggagtg cccggtgttc
       61 gagcctagct gggccgagtt ccgagaccct cttggctaca tcgcgaaaat caggcccatc
      121 gcagagaaat cgggcatttg caagatccgc ccacccgcgg actggcagcc accctttgct
      181 gtggaagtgg acaacttcag gtttaccccc cgaatccaga ggctgaatga gctagaggcc
      241 cagacgagag tgaaactgaa ctacttggac cagattgcca aattctggga aatccagggc
      301 tcctccttaa agattcccaa tgtagaacgg cggatcttgg acctctacag tctcagcaaa
      361 attgtggtgg aggaaggtgg ttatgaagct atctgcaagg accgtcggtg ggctcgggta
      421 gcccagcgcc tcaactatcc accaggcaaa aatattggct ccttgctacg ctcccactac
      481 gaacgcattg tttatcccta tgaaatgtac cagtctggag ccaaccttgt gcagtgtaac
      541 acacgtccat ttgataatga ggagaaggac aaggaataca aaccccacag catcccccta
      601 cgacagtctg tgcagccttc caagttcaac agctatggcc ggcgggccaa gagactgcag
      661 cctgatccgg aacccacaga ggaagacatt gagaagaatc cagagctgaa aaagctacag
      721 atctatgggg caggccccaa gatgatgggc ctgggcctca tggccaaaga caagactctg
      781 cggaagaaag ataaggaggg gcctgagtgt ccccccacag tagtggtgaa ggaggagtta
      841 ggtggggatg tgaaggtgga gtcaacatcg cctaagacct tcctggagag caaggaggag
      901 ctgagtcaca gcccagaacc ctgcaccaag atgaccatga ggctacggag gaaccacagc
      961 aatgcccagt ttattgagtc atatgtctgc cggatgtgtt ctcgagggga tgaggatgac
     1021 aagctcctgc tgtgtgatgg ctgtgatgac aactaccaca tcttctgcct gctgcctcct
     1081 ctgcctgaga tccccaaggg tgtctggcgg tgcccaaagt gtgtcatggc ggagtgtaag
     1141 cggcccccag aagcctttgg ctttgagcag gctacccggg aatacactct gcagagcttt
     1201 ggcgagatgg ccgactcctt taaagctgac tacttcaaca tgcccgtgca tatggtgccc
     1261 acagaacttg tggagaagga gttctggagg ctggtaaata gcattgagga agatgtgact
     1321 gttgagtatg gagctgacat ccattccaaa gaatttggca gcggtttccc tgtcagtgac
     1381 agtaaacggc acctaacccc cgaagaggag gagtatgcta ccagtggttg gaacctaaat
     1441 gtgatgccgg tgttggaaca gtctgtactg tgccacatca atgcagatat ctctggcatg
     1501 aaggtgccct ggctctacgt gggcatggtc ttctcagcct tttgctggca tattgaggat
     1561 cactggagtt actccattaa ctacctccac tggggtgagc cgaagacctg gtatggggtg
     1621 ccctcacttg cagcagaaca tttggaagaa gtgatgaaga agctgacacc tgaactattt
     1681 gatagccagc ctgacctcct gcaccaactt gtcaccctca tgaatcccaa caccctcatg
     1741 tcccatggtg tgccagttgt ccgcacaaac cagtgtgcag gagagtttgt catcaccttc
     1801 ccccgtgctt accacagcgg cttcaaccaa ggctacaact ttgccgaggc tgtcaacttt
     1861 tgcactgctg actggttgcc tgctgggcgc cagtgcattg agcactaccg ccggctccgg
     1921 agatactgcg tcttctccca tgaggagctt atctgcaaga tggctgcctg cccagagaag
     1981 ctagacctga acctggcggc agctgtgcat aaggagatgt tcatcatggt gcaagaagag
     2041 cggcgtctac gaaaggccct gctggagaag ggtatcacag aggctgagcg agaggctttc
     2101 gagctgctcc cagatgatga gcgccagtgt atcaagtgca agactacgtg tttcctgtca
     2161 gccctggcct gctacgactg cccagacggc cttgtctgcc tttcccacat caatgatctc
     2221 tgcaagtgct ccagtagccg gcagtacctg cggtatcggt ataccttgga tgagcttcct
     2281 gccatgctgc ataagctgaa ggttcgggct gagtcctttg acacctgggc caacaaagtg
     2341 cgagtggccc tggaggtgga ggatgggcgg aagcgcagcc ttgaagaact gagggcacta
     2401 gagtctgaag cccgtgagcg gaggtttcct aatagtgagc tgctgcagca actaaagaac
     2461 tgcctgagtg aggcagaggc ttgcgtgtcc cgagctctgg gactggtcag cggccaggaa
     2521 gctggccccc acagggtggc tggtctacag atgaccctga ctgagctccg ggcctttctg
     2581 gaccagatga acaacctgcc ttgcgccatg caccagattg gggatgtcaa gggtgttctg
     2641 gaacaggtgg aggcctacca ggctgaggct cgtgaggccc tggcctcact gccctccagt
     2701 ccagggctac tgcagtccct gttggagagg gggcggcagc tgggggtgga ggtgcctgag
     2761 gcccagcagc tccagcggca ggtggaacag gcgcgatggc tggatgaggt gaaacgcaca
     2821 ctggccccct cagcccgaag gggcaccttg gctgtcatgc gaggactgtt ggtcgcgggt
     2881 gccagtgtag cccctagccc tgctgtggat aaagcccagg ccgagctgca ggaactgctg
     2941 accattgctg aacgctggga ggagaaagcc cacctctgcc tggaggccag gcagaagcat
     3001 ccaccagcca cacttgaggc cataatccgt gaagcggaaa acatccctgt tcacctgccc
     3061 aacatccagg ctctcaagga ggctcttgct aaggcccggg cctggattgc tgatgttgat
     3121 gagatccaaa atggtgacca ctacccctgc ctggatgact tggagggcct agtagctgtg
     3181 ggccgggacc tacctgtggg gctggaggag ctgagacagc tagagctaca ggtactgaca
     3241 gcgcactcct ggagggagaa ggcctccaag accttcctca agaaaaattc ttgctacacg
     3301 ctgctggagg ttctctgccc atgtgcagat gccggctcag acagcaccaa gcgcagccgg
     3361 tggatggaga aggagctggg gttgtacaaa tctgacacag agctgctggg gctgtctgcg
     3421 caggacctca gggacccagg ctctgtgatc gtggccttca aggaggggga acagaaggag
     3481 aaggagggta tcctgcagct gcgtcgcacc aattcggcca agcccagtcc actggcatca
     3541 tcgagcacgg cctcctctac aacctctatc tgtgtgtgtg ggcaggtgct ggctggggcg
     3601 ggagctctgc agtgtgacct gtgtcaggac tggttccatg ggcggtgtgt gtcagtgcct
     3661 cgcctcctca gctctccgag gcccaatccc acctcatccc cactgctggc ctggtgggaa
     3721 tgggacacca aattcctgtg tccactgtgt atgcgctcaa ggcgcccgcg cctggagacc
     3781 atcctggcac tgctggtagc cctgcagaga ctgcctgtgc ggctgcccga gggcgaggcc
     3841 ctgcagtgcc tcacagagag ggccatcagc tggcaaggcc gcgccaggca ggctctggcc
     3901 tctgaagatg tgactgctct tttgggacgg ctggctgagc tccgccaacg gctacaggct
     3961 gaacctagac ctgaggagcc tcctaactac cctgcagccc ctgcttctga ccccctcaga
     4021 gagggcagtg gcaaggatat gcctaaggtc cagggcttac tggagaatgg agacagtgtg
     4081 accagtcctg agaaggtagc cccggaggag ggctcaggta agagagatct ggagctgctg
     4141 tcctcgctgt tgccacagtt gactggccct gtgttggaac tgcctgaggc aacccgggcc
     4201 cccttggagg agctcatgat ggagggggac ctgctcgagg tgaccctgga tgagaaccac
     4261 agcatatggc agctgctgca ggctggacag cccccagacc tggagaggat ccgcacactt
     4321 ctggagctgg agaaggcaga gcgtcacggg agtcgggctc ggggccgggc cctggagagg
     4381 cggcggcggc ggaaggtgga tcggggtggg gagggcgatg acccagcccg agaggagcta
     4441 gagccaaaga gggtacggag ctcagggcca gaggctgagg aggtccagga ggaggaagag
     4501 ctggaggagg agactggggg tgagggcccc cctgcaccca tccccaccac tggcagcccc
     4561 agcacccagg agaaccagaa tggcttggaa ccggcggaag ggaccacttc aggcccctcg
     4621 gcccctttct ccactctgac tccccggctg catctgccct gcccacagca gccgcctcag
     4681 caacagttgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     4741 atatcttgtg gaaaggacga tccatcccta ggccttgaac aaag
//
