LOCUS       sequence-28486-e         916 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Berndt JA, Kim JG, Hudson LD
  TITLE     Identification of cis-regulatory elements in the myelin proteolipid 
            protein (PLP) gene.
  JOURNAL   J Biol Chem. 1992 Jul 25. 267(21):14730-7.
  PUBMED    1378839
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1992 Jul 25. 267(21):14730-7."
COMMENT     Sequence Label: pJB052
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    409..465
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(474..490)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(474..490)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(487..509)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    498..514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(522..528,529..546,547..552))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    567..588
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     657..676
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(757..774)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acggtggtat atccngtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gccaagcttc tggtctctcc agcctccttc ttcggtcttt tactttttct ctctgagtat
      121 ctttgtcctc ttctcctgca atgaaaaggg ctttctgatt gacagccaac ccccttaaag
      181 ggacaggcct gctttcctcc tcctctcctc ctccccttct cctccccttt tccccaccta
      241 ctcagggata ctcccttctc taagggctcc atcttctctt tctgcctttc actgccaatt
      301 gtttcatttt ctttgttttt ccttttttcc ctaaggcttg cctggaattt cgctttgctg
      361 cagagacatc tggaaatgtg gattgagggt ggaaataaaa tgaagatgaa gcttgcatgc
      421 ctgcaggtcg actctagagg atccccgggt accgagctcg aattcgtaat catgtcatag
      481 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      541 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      601 tcactgcccg ctttccagtc gggaaacctg tcgtgccaga tactggctta actatgcggc
      661 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
      721 aaggagaaaa taccgcatca ngcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      781 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      841 agaatcnngg ataacgcnnn aagaacatgt gancaaaggn cagcaaaggc ngaaccgtaa
      901 aaggccgcgt tgctgg
//
