LOCUS       sequence-284913-        5171 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0951
FEATURES             Location/Qualifiers
     source          1..5171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggttccaa gagaagaaga gctacctccc cttccagcag tgtcagcggg
       61 gactttgatg atgggcacca ttctgtgtca acaccaggcc caagcaggaa aaggaggaga
      121 ctttccaatc ttccaactgt agatcctatt gccgtgtgcc atgaactcta taataccatc
      181 cgagactata aggatgaaca gggcagactt ctctgtgagc tcttcattag ggcaccaaag
      241 cgaagaaatc aaccagacta ttatgaagtg gtttctcagc ccattgactt gatgaaaatc
      301 caacagaaac taaaaatgga agagtatgat gatgttaatt tgctgactgc tgacttccag
      361 cttcttttta acaatgcaaa gtcctattat aagccagatt ctcctgaata taaagccgct
      421 tgcaaactct gggatttgta ccttcgaaca agaaatgagt ttgttcagaa aggagaagca
      481 gatgacgaag atgatgatga agatgggcaa gacaatcagg gcacagtgac tgaaggatct
      541 tctccagctt acttgaagga gatcctggag cagcttcttg aagccatagt tgtagctaca
      601 aatccatcag gacgtctcat tagcgaactt tttcagaaac tgccttctaa agtgcaatat
      661 ccagattatt atgcaataat taaggagcct atagatctca agaccattgc ccagaggata
      721 cagaatggaa gctacaaaag tattcatgca atggccaaag atatagatct cctcgcaaaa
      781 aatgccaaaa cttataatga gcctggctct caagtattca aggatgcaaa ttcaattaaa
      841 aaaatatttt atatgaaaaa ggctgaaatt gaacatcatg aaatggctaa gtcaagtctt
      901 cgaatgagga ctccatccaa cttggctgca gccagactga caggtccttc acacagtaaa
      961 ggcagccttg gtgaagagag aaatcccact agcaagtatt accgtaataa aagagcagta
     1021 caaggaggtc gtttatcagc aattacaatg gcacttcaat atggctcaga aagtgaagaa
     1081 gatgctgctt tagctgctgc acgctatgaa gagggagagt cagaagcaga aagcatcact
     1141 tcctttatgg atgtttcaaa tcctttttat cagctttatg acacagttag gagttgtcgg
     1201 aataaccaag ggcagctaat agctgaacct ttttaccatt tgccttcaaa gaaaaaatac
     1261 cctgattatt accagcaaat taaaatgccc atatcactac aacagatccg aacaaaactg
     1321 aagaatcaag aatatgaaac tttagatcat ttggagtgtg atctgaattt aatgtttgaa
     1381 aatgccaaac gctataatgt gcccaattca gccatctaca agcgagttct aaaattgcag
     1441 caagttatgc aggcaaagaa gaaagagctt gccaggagag acgatatcga ggacggagac
     1501 agcatgatct cttcagccac ctctgatact ggtagtgcca aaagaaaaag taaaaagaac
     1561 ataagaaagc agcgaatgaa aatcttattc aatgttgttc ttgaagctcg agagccaggt
     1621 tcaggcagaa gactttgtga cctatttatg gttaaaccat ccaaaaagga ctatcctgat
     1681 tattataaaa tcatcttgga gccaatggac ttgaaaataa ttgagcataa catccgcaat
     1741 gacaaatatg ctggtgaaga gggaatgata gaagacatga agctgatgtt ccggaatgcc
     1801 aggcactata atgaggaggg ctcccaggtt tataatgatg cacatatcct ggagaagtta
     1861 ctcaaggaga aaaggaaaga gctgggccca ctgcctgatg atgatgacat ggcttctccc
     1921 aaactcaagc tgagtaggaa gagtggcatt tctcctaaaa aatcaaaata catgactcca
     1981 atgcagcaga aactaaatga ggtctatgaa gctgtaaaga actatactga taagaggggt
     2041 cgccgcctca gtgccatatt tctgaggctt ccctctagat ctgagttgcc tgactactat
     2101 ctgactatta aaaagcccat ggacatggaa aaaattcgaa gtcacatgat ggccaacaag
     2161 taccaagata ttgactctat ggttgaggac tttgtcatga tgtttaataa tgcctgtaca
     2221 tacaatgagc cggagtcttt gatctacaaa gatgctcttg ttctacacaa agtcctgctt
     2281 gaaacacgca gagacctgga gggagatgag gactctcatg tcccaaatgt gactttgctg
     2341 attcaagagc ttatccacaa tctttttgtg tcagtcatga gtcatcagga tgatgaggga
     2401 agatgctaca gcgattcttt agcagaaatt cctgctgtgg atcccaactt tcctaacaaa
     2461 ccacccctta catttgacat aattaggaag aatgttgaaa ataatcgcta ccgtcggctt
     2521 gatttatttc aagagcatat gtttgaagta ttggaacgag caagaaggat gaatcggaca
     2581 gattcagaaa tatatgaaga tgcagtagaa cttcagcagt tttttattaa aattcgtgat
     2641 gaactctgca aaaatggaga gattcttctt tcaccggcac tcagctatac cacaaaacat
     2701 ttgcataatg atgtggagaa agagagaaag gaaaaattgc caaaagaaat agaggaagat
     2761 aaactaaaac gagaagaaga aaaaagagaa gctgaaaaga gtgaagattc ctctggtgct
     2821 gcaggcctct caggcttaca tcgcacatac agccaggact gtagctttaa aaacagcatg
     2881 taccatgttg gagattacgt ctatgtggaa cctgcagagg ccaacctaca accacatatc
     2941 gtctgtattg aaagactgtg ggaggattca gctggtgaaa aatggttgta tggctgttgg
     3001 ttttaccgac caaatgaaac attccacctg gctacacgaa aatttctaga aaaagaagtt
     3061 tttaagagtg actattacaa caaagttcca gttagtaaaa ttctaggcaa gtgtgtggtc
     3121 atgtttgtca aggaatactt taagttatgc ccagaaaact tccgagatga ggatgttttt
     3181 gtctgtgaat cacggtattc tgccaaaacc aaatctttta agaaaattaa actgtggacc
     3241 atgcccatca gctcagtcag gtttgtccct cgggatgtgc ctctgcctgt ggttcgcgtg
     3301 gcctctgtat ttgcaaatgc agataaaggt gatgatgaga agaatacaga caactcagag
     3361 gacagtcgag ctgaagacaa ttttaacttg gaaaaggaaa aagaagatgt ccctgtggaa
     3421 atgtccaatg gtgaaccagg ttgccactac tttgagcagc tccattacaa tgacatgtgg
     3481 ctgaaggttg gcgactgtgt cttcatcaag tcccatggcc tggtgcgtcc tcgtgtgggc
     3541 agaattgaaa aagtatgggt tcgagatgga gctgcatatt tttatggccc catcttcatt
     3601 cacccagaag aaacagagca tgagcccaca aaaatgttct acaaaaaaga agtatttctg
     3661 agtaatctgg aagaaacctg ccccatgaca tgtattctcg gaaagtgtgc tgtgttgtca
     3721 ttcaaggact tcctctcctg caggccaact gaaataccag aaaatgacat tctgctttgt
     3781 gagagccgct acaatgagag cgacaagcag atgaagaaat tcaaaggatt gaagaggttt
     3841 tcactctctg ctaaagtggt agatgatgaa atttactact tcagaaaacc aattgttcct
     3901 cagaaggagc catcaccttt gctggaaaag aagatccagt tgctagaagc taaatttgcc
     3961 gagttagaag gtggagatga tgatattgaa gagatgggag aagaagatag tgaggtcatt
     4021 gaacctcctt ctctacctca gcttcagacc cccctggcca gtgagctgga cctcatgccc
     4081 tacacacccc cacagtctac cccaaagtct gccaaaggca gtgcaaagaa ggaaggctcc
     4141 aaacggaaaa tcaacatgag tggctacatc ctgttcagca gtgagatgag ggctgtgatt
     4201 aaggcccaac acccagacta ctctttcggg gagctcagcc gcctggtggg gacagaatgg
     4261 agaaatcttg agacagccaa gaaagcagaa tatgaagagc gggcagctaa agttgctgag
     4321 cagcaggaga gagagcgagc agcacagcaa cagcagccga gtgcttctcc ccgagcaggc
     4381 acccctgtgg gggctctcat gggggtggtg ccaccaccaa caccaatggg gatgctcaat
     4441 cagcagttga cacctgttgc aggcatgatg ggtggctatc cgccaggcct tccacctttg
     4501 cagggcccag ttgatggcct tgttagcatg ggcagcatgc agccacttca ccctgggggg
     4561 cctccacccc accatcttcc gccaggtgtg cctggcctcc cgggcatccc accaccgggt
     4621 gtgatgaacc aaggagtggc ccctatggta gggactccag caccaggtgg aagtccatat
     4681 ggacaacagg tgggagtttt ggggcctcca gggcagcagg caccacctcc atatcccggc
     4741 ccacatccag ctggaccccc tgtcatacag cagccaacaa cacccatgtt tgtagctccc
     4801 ccaccaaaga cccagcggct tcttcactca gaggcctacc tgaaatacat tgaaggactc
     4861 agtgcggagt ccaacagcat tagcaagtgg gatcagacac tggcagctcg aagacgcgac
     4921 gtccatttgt cgaaagaaca ggagagccgc ctaccctctc actggctgaa aagcaaaggg
     4981 gcccacacca ccatggcaga tgccctctgg cgccttcgag atttgatgct ccgggacacc
     5041 ctcaacattc gccaagcata caacctagaa aatgtttagt aatgaactag tccgtaactt
     5101 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgatgc gagtcctaga
     5161 tgactatacc t
//
