LOCUS       sequence-285108-        5258 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2279
FEATURES             Location/Qualifiers
     source          1..5258
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 accgccacca tgaatcagaa cactactgag cctgtggcgg ccaccgagac cctggctgag
       61 gtacccgaac atgtgctgcg aggacttccg gaggaagtga ggcttttccc ttctgctgtt
      121 gacaagaccc ggattggtgt ctgggccact aaaccaattt taaaaggcaa aaaatttggg
      181 ccatttgttg gtgataagaa aaaaagatct caggttaaga ataatgtata catgtgggag
      241 gtgtattacc caaatttggg atggatgtgc attgatgcca ctgatccaga gaagggaaac
      301 tggctgcgat atgtgaattg ggcttgctca ggagaagagc aaaatttatt cccactggaa
      361 atcaacagag ccatttacta taaaacttta aagccaatcg cgccgggcga ggagctcctg
      421 gtctggtaca atggggaaga caaccctgag atagcagctg cgattgagga agagcgagcc
      481 agcgcccgga gcaagcggag ctcccccaag agccggaaag ggaagaaaaa atcccaggaa
      541 aataaaaaca aaggaaacaa aatccaagac atacaactga agacaagtga gccagatttc
      601 acctctgcaa atatgagaga ttctgcagaa ggtcctaaag aagacgaaga gaagccttca
      661 gcctcagcac ttgagcagcc ggccaccctc caggaggtgg ccagtcagga ggtgcctcca
      721 gaactagcaa cccctgcccc tgcctgggag ccacagccag aaccagacga gcgattagaa
      781 gcggcagctt gtgaggtgaa tgatttgggg gaagaggagg aggaggaaga ggaggaggat
      841 gaagaagaag aagaagatga tgatgatgat gagttggaag acgaggggga agaagaagcc
      901 agcatgccaa atgaaaattc tgtgaaagag ccagaaatac ggtgtgatga gaagccagaa
      961 gatttattag aggaaccaaa aacaacttca gaagaaactc ttgaagactg ctcagaggta
     1021 acacctgcca tgcaaatccc cagaactaaa gaagaggcca atggtgatgt atttgaaacg
     1081 tttatgtttc cgtgtcaaca ttgtgaaagg aagtttacaa ccaaacaggg gcttgagcgt
     1141 cacatgcata tccatatatc caccgtcaat catgctttca aatgcaagta ctgtgggaaa
     1201 gcctttggca cacagattaa ccggcggcga catgagcggc gccatgaagc agggttaaag
     1261 cggaaaccca gccaaacact acagccgtca gaggatctgg ctgatggcaa agcatctgga
     1321 gaaaacgttg cttcaaaaga tgattcgagt cctcccagtc ttgggccaga ctgtctgatc
     1381 atgaattcag agaaggcttc ccaagacaca ataaattctt ctgtcgtaga agagaatggg
     1441 gaagttaaag aacttcatcc gtgcaaatat tgtaaaaagg tttttggaac tcatactaat
     1501 atgagacggc atcagcgtag agttcacgaa cgtcatctga ttcccaaagg tgtacggcga
     1561 aaaggaggcc ttgaagagcc ccagcctcca gcagaacagg cccaggccac ccagaacgtg
     1621 tatgtaccaa gcacagagcc ggaggaggaa ggggaagcag atgatgtgta catcatggac
     1681 atttctagca atatctctga aaacttaaat tactatattg atggtaaaat tcaaactaat
     1741 aacaacacta gtaactgtga tgtgattgag atggagtctg cttcggcaga tttgtatggt
     1801 ataaattgtc tgctcactcc agttacagtg gaaattactc aaaatataaa gaccacacag
     1861 gtccctgtaa cagaagatct tcctaaagag cctttgggca gcacaaatag tgaggccaag
     1921 aagcggagaa ctgcgagccc acctgcactg cccaaaatta aggccgaaac agactctgac
     1981 cccatggtcc cctcttgctc tttaagtctt cctcttagca tatcaacaac agaggcagtg
     2041 tctttccaca aagagaaaag tgtttatttg tcatcaaagc tcaaacaact tcttcaaacc
     2101 caagataaac taactcctgc agggatttca gcaactgaaa tagctaaatt aggtcctgtt
     2161 tgtgtgtctg ctcctgcatc aatgttgcct gtgacctcaa gtaggtttaa gaggcggacc
     2221 agctctcctc ccagttctcc acagcacagt cctgcccttc gagactttgg aaagccaagt
     2281 gatgggaaag cagcatggac cgatgccggg ctgacttcca aaaaatccaa attagaaagt
     2341 cacagcgact caccagcatg gagtttgtct gggagagatg agagagaaac tgtgagccct
     2401 ccatgctttg atgaatataa aatgtctaaa gagtggacag ctagttctgc ttttagcagt
     2461 gtgtgcaacc agcagccact ggatttatcc agcggtgtca aacagaaggc tgagggtaca
     2521 ggcaagactc cagtccagtg ggaatctgtc ttagatctca gtgtgcataa aaagcattgt
     2581 agtgactctg aaggcaagga attcaaagaa agtcattcag tgcagcctac gtgtagtgct
     2641 gtaaagaaaa ggaaaccaac cacctgcatg ctgcagaagg ttcttctcaa tgaatataat
     2701 ggcatcgatt tacctgtaga aaaccctgca gatgggacca ggagcccaag tccttgtaaa
     2761 tccctagaag ctcagccaga tcctgacctc ggtccgggct ctggtttccc tgcccctact
     2821 gttgagtcca cacctgatgt ttgtccttca tcacctgccc tgcagacacc ctccctttca
     2881 tccggtcagc tgcctcctct cttgatcccc acagatccct cttcccctcc accctgtccc
     2941 ccggtattaa ctgttgccac tccgccccct cccctccttc ctaccgtacc tcttccagcc
     3001 ccctcttcca gtgcatctcc acacccatgc ccctctccac tctcaaatgc caccgcacag
     3061 tccccacttc caattctgtc cccaacagtg tccccctctc cctctcccat tcctcccgtg
     3121 gagcccctga tgtctgccgc ctcacccggg cctccaacac tttcttcttc ctcctcttca
     3181 tcttcctcct cctcttcgtt ttcttcttca tcttcctcct cttctccttc tccacctcct
     3241 ctctccgcaa tatcatctgt tgtttcctct ggtgataatc tggaggcttc tctccccatg
     3301 atatctttca aacaggagga attagagaat gaaggtctga aacccaggga agagccccag
     3361 tctgctgctg aacaggatgt tgttgttcag gaaacattca acaaaaactt tgtttgcaac
     3421 gtctgtgaat caccttttct ttccattaaa gatctaacca aacatttatc tattcatgct
     3481 gaagaatggc ccttcaaatg tgaattttgt gtgcagcttt ttaaggataa aacggacttg
     3541 tcagaacatc gctttttgct tcatggagtt gggaatatct ttgtgtgttc tgtttgtaaa
     3601 aaagaatttg cttttttgtg caatttgcag cagcaccagc gagatctcca cccagataag
     3661 gtgtgcacac atcacgagtt tgaaagcggg actctgaggc cccagaactt tacagatccc
     3721 agcaaggccc atgtagagca tatgcagagc ttgccagaag atcctttaga aacttctaaa
     3781 gaagaagagg agttaaatga ttcctctgaa gagctttaca cgactataaa aataatggct
     3841 tctggaataa agacaaaaga tccagatgtt cgattgggcc tcaatcagca ttacccaagc
     3901 tttaaaccac ctccatttca gtaccatcac cgtaacccca tggggattgg tgtgacagcc
     3961 acaaatttca ctacacacaa tattccacag actttcacta ccgccattcg ctgcacaaag
     4021 tgtggaaaag gtgtcgacaa tatgccggag ttgcacaaac atatcctggc ttgtgcttct
     4081 gcaagtgaca agaagaggta cacgcctaag aaaaacccag taccattaaa acaaactgtg
     4141 caacccaaaa atggcgtggt ggttttagat aactctggga aaaatgcctt ccgacgaatg
     4201 ggacagccca aaaggcttaa ctttagtgtt gagctcagca aaatgtcgtc gaataagctc
     4261 aaattaaatg cattgaagaa aaaaaatcag ctagtacaga aagcaattct tcagaaaaac
     4321 aaatctgcaa agcagaaggc cgacttgaaa aatgcttgtg agtcatcctc tcacatctgc
     4381 ccttactgta atcgagagtt cacttacatt ggaagcctga ataaacacgc cgccttcagc
     4441 tgtcccaaaa aacccctttc tcctcccaaa aaaaaagttt ctcattcatc taagaaaggt
     4501 ggacactcat cacctgcaag tagtgacaaa aacagtaaca gcaaccaccg cagacggaca
     4561 gcggatgcgg agattaaaat gcaaagcatg cagactccgt tgggcaagac cagagcccgc
     4621 agctcaggcc ccacccaagt cccacttccc tcctcatcct tcaggtccaa gcagaacgtc
     4681 aagtttgcag cttcggtgaa atccaaaaaa ccaagctcct cctctttaag gaactccagc
     4741 ccgataagaa tggccaaaat aactcatgtt gaggggaaaa aacctaaagc tgtggccaag
     4801 aatcattctg ctcagctttc cagcaaaaca tcacggagcc tgcacgtgag ggtacagaaa
     4861 agcaaagctg ttttacaaag caaatccacc ttggcgagta agaaaagaac agaccggttc
     4921 aatataaaat ctagagagcg gagtgggggg ccagtcaccc ggagccttca gctggcagct
     4981 gctgctgact tgagtgagaa caagagagag gacggcagcg ccaagcagga gctgaaggac
     5041 ttcagctaca gcctccgctt ggcgtcccga tgctctccac cagcggcccc gtacatcacc
     5101 aggcagtata ggaaggtcaa agctccagct gcagcccagt tccagggacc attcttcaaa
     5161 gagtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     5221 tgtggaaagg acgagacgtg tgatcccagc atgtacct
//
