LOCUS       sequence-285165-         638 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2725
FEATURES             Location/Qualifiers
     source          1..638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagatccc tgccccggag cccgagaaga caggaatcgg tgagtttcaa
       61 ggatgtggct gtggacttca cccaggagga gtggggtcaa ctagactccc ctcagagggc
      121 cttgtaccgg gatgtgatgt tggagaacta ccagaacctt cttgccctag gacctccact
      181 gcacaagcca gatgtgatct ctcatctgga acgaggcgag gagccatgga gcatgcagag
      241 ggaagtcccc agagggccct gtccagaatg ggagctgaag gcggtgccct ctcaacagca
      301 gggcatttgc aaagaagaac cggcccagga gcccatcatg gagcggcccc tcggcggggc
      361 gcaggcgtgg gggcgccagg caggtgctct gcagaggagt caggctgcgc cctgggcgcc
      421 cgcacctgcc atggtctggg acgtccctgt agaggaattc cccctcaggt gtcccctctt
      481 cgcccagcaa cgcgttcccg aggggggacc cttgctggac acacgcaaga acgtccaggc
      541 cactagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      601 tgtggaaagg acgaatagat agaaagtgct acttatca
//
