LOCUS       sequence-285197-       15910 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15910)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0702
FEATURES             Location/Qualifiers
     source          1..15910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    11767..12355
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11820..11840)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12238..12249)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12427..12660
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12738..12859
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12775..12794)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12829..12848
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12899..13034
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13054..13073)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13055..13073)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13083..13100)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13083..13099)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13092..13114)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13241..13696
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13328..13347)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13538..13559
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13722..13826
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13827..13895,13896..14687)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14045..14064)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14858..15446
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15347..15366
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15600..15617
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    15734..15755
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15770..15775,15776..15793,15794..15800)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15808..15824
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15813..15835
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15832..15848
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15832..15848
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     15867..15887
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        15869..15887
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtcg ggatccacac agcctgtggc
     4141 acagacgtgg agggccactg agccccgcta cccgccccac agcctttcct acccagtgca
     4201 gatcgcccgg acgcacacgg acgtcgggct cctggagtac cagcaccact cccgcgacta
     4261 tgcctcccac ctgtcgcccg gctccatcat ccagccccag cggcggaggc cctccctgct
     4321 gtctgagttc cagcccggga atgaacggtc ccaggagctc cacctgcggc cagagtccca
     4381 ctcatacctg cccgagctgg ggaagtcaga gatggagttc attgaaagca agcgccctcg
     4441 gctagagctg ctgcctgacc ccctgctgcg accgtcaccc ctgctggcca cgggccagcc
     4501 tgcgggatct gaagacctca ccaaggaccg tagcctgacg ggcaagctgg aaccggtgtc
     4561 tccccccagc cccccgcaca ctgaccctga gctggagctg gtgccgccac ggctgtccaa
     4621 ggaggagctg atccagaaca tggaccgcgt ggaccgagag atcaccatgg tagagcagca
     4681 gatctctaag ctgaagaaga agcagcaaca gctggaggag gaggctgcca agccgcccga
     4741 gcctgagaag cccgtgtcac cgccgcccat cgagtcgaag caccgcagcc tggtgcagat
     4801 catctacgac gagaaccgga agaaggctga agctgcacat cggattctgg aaggcctggg
     4861 gccccaggtg gagctgccgc tgtacaacca gccctccgac acccggcagt atcatgagaa
     4921 catcaaaata aaccaggcga tgcggaagaa gctaatcttg tacttcaaga ggaggaatca
     4981 cgctcggaaa caatgggagc agaagttctg ccagcgctat gaccagctca tggaggcctg
     5041 ggagaagaag gtggagcgca tcgagaacaa cccccggcgg cgggccaagg agagcaaggt
     5101 gcgcgagtac tacgagaagc agttccctga gatccgcaag cagcgcgagc tgcaggagcg
     5161 catgcagagc agggtgggcc agcggggcag tgggctgtcc atgtcggccg cccgcagcga
     5221 gcacgaggtg tcagagatca tcgatggcct ctcagagcag gagaacctgg agaagcagat
     5281 gcgccagctg gccgtgatcc cgcccatgct gtacgacgct gaccagcagc gcatcaagtt
     5341 catcaacatg aacgggctta tggccgaccc catgaaggtg tacaaagacc gccaggtcat
     5401 gaacatgtgg agtgagcagg agaaggagac cttccgggag aagttcatgc agcatcccaa
     5461 gaactttggc ctgatcgcat cattcctgga gaggaagaca gtggctgagt gcgtcctcta
     5521 ttactacctg actaagaaga atgagaacta taagagcctg gtgagacgga gctatcggcg
     5581 ccgcggcaag agccagcagc agcaacaaca gcagcagcag cagcagcagc agcagcagca
     5641 gcagcccatg ccccgcagca gccaggagga gaaagatgag aaggagaagg aaaaggaggc
     5701 ggagaaggag gaggagaagc cggaggtgga gaacgacaag gaagacctcc tcaaggagaa
     5761 gacagacgac acctcagggg aggacaacga cgagaaggag gctgtggcct ccaaaggccg
     5821 caaaactgcc aacagccagg gaagacgcaa aggccgcatc acccgctcaa tggctaatga
     5881 ggccaacagc gaggaggcca tcacccccca gcagagcgcc gagctggcct ccatggagct
     5941 gaatgagagt tctcgctgga cagaagaaga aatggaaaca gccaagaaag gtctcctgga
     6001 acacggccgc aactggtcgg ccatcgcccg gatggtgggc tccaagactg tgtcgcagtg
     6061 taagaacttc tacttcaact acaagaagag gcagaacctc gatgagatct tgcagcagca
     6121 caagctgaag atggagaagg agaggaacgc gcggaggaag aagaagaaag cgccggcggc
     6181 ggccagcgag gaggctgcat tcccgcccgt ggtggaggat gaggagatgg aggcgtcggg
     6241 cgtgagcgga aatgaggagg agatggtgga ggaggctgaa gccttacatg cctctgggaa
     6301 tgaggtgccc agaggggaat gcagtggccc agccactgtc aacaacagct cagacaccga
     6361 gagcatcccc tctcctcaca ctgaggccgc caaggacaca gggcagaatg ggcccaagcc
     6421 cccagccacc ctgggcgccg acgggccacc cccagggcca cccaccccac caccggagga
     6481 catcccggcc cccactgagc ccaccccggc ctctgaagcc accggagccc ctacgccccc
     6541 accagcaccc ccatcgccct ctgcacctcc tcctgtggtc cccaaggagg agaaggagga
     6601 ggagaccgca gcagcgcccc cagtggagga gggggaggag cagaagcccc ccgcggctga
     6661 ggagctggca gtggacacag ggaaggccga ggagcccgtc aagagcgagt gcacggagga
     6721 agccgaggag gggccggcca agggcaagga cgcggaggcc gctgaggcca cggccgaggg
     6781 ggcgctcaag gcagagaaga aggagggcgg gagcggcagg gccaccacag ccaagagctc
     6841 gggcgccccc caggacagcg actccagtgc tacctgcagt gcagacgagg tggatgaggc
     6901 cgagggcggc gacaagaacc ggctgctgtc cccaaggccc agcctcctca ccccgactgg
     6961 cgacccccgg gccaatgcct caccccagaa gccactggac ctgaagcagc tgaagcagcg
     7021 agcggctgcc atccccccca tccaggtcac caaagtccat gagccccccc gggaggacgc
     7081 agctcccacc aagccagctc ccccagcccc accgccaccg caaaacctgc agccggagag
     7141 cgacgcccct cagcagcctg gcagcagccc ccggggcaag agcaggagcc cggcaccccc
     7201 cgccgacaag gaggccttcg cagccgaggc ccagaagctg cctggggacc ccccttgctg
     7261 gacttccggc ctgcccttcc ccgtgccccc ccgtgaggtg atcaaggcct ccccgcatgc
     7321 cccggacccc tcagccttct cctacgctcc acctggtcac ccactgcccc tgggcctcca
     7381 tgacactgcc cggcccgtcc tgccgcgccc acccaccatc tccaacccgc ctcccctcat
     7441 ctcctctgcc aagcacccca gcgtcctcga gaggcaaata ggtgccatct cccaaggaat
     7501 gtcggtccag ctccacgtcc cgtactcaga gcatgccaag gccccggtgg gccctgtcac
     7561 catggggctg cccctgccca tggaccccaa aaagctggca cccttcagcg gagtgaagca
     7621 ggagcagctg tccccacggg gccaggctgg gccaccggag agcctggggg tgcccacagc
     7681 ccaggaggcg tccgtgctga gagggacagc tctgggctca gttccgggcg gaagcatcac
     7741 caaaggcatt cccagcacac gggtgccctc ggacagcgcc atcacatacc gcggctccat
     7801 cacccacggc acgccagctg acgtcctgta caagggcacc atcaccagga tcatcggcga
     7861 ggacagcccg agtcgcttgg accgcggccg ggaggacagc ctgcccaagg gccacgtcat
     7921 ctacgaaggc aagaagggcc acgtcttgtc ctatgagggt ggcatgtctg tgacccagtg
     7981 ctccaaggag gacggcagaa gcagctcagg acccccccat gagacggccg cccccaagcg
     8041 cacctatgac atgatggagg gccgcgtggg cagagccatc tcctcagcca gcatcgaagg
     8101 tctcatgggc cgtgccatcc cgccggagcg acacagcccc caccacctca aagagcagca
     8161 ccacatccgc gggtccatca cacaagggat ccctcggtcc tacgtggagg cacaggagga
     8221 ctacctgcgt cgggaggcca agctcctaaa gcgggagggc acgcctccgc ccccaccgcc
     8281 ctcacgggac ctgaccgagg cctacaagac gcaggccctg ggccccctga agctgaagcc
     8341 ggcccatgag ggcctggtgg ccacggtgaa ggaggcgggc cgctccatcc atgagatccc
     8401 gcgcgaggag ctgcggcaca cgcccgagct gcccctggcc ccgcggccgc tcaaggaggg
     8461 ctccatcacg cagggcaccc cgctcaagta cgacaccggc gcgtccacca ctggctccaa
     8521 aaagcacgac gtacgctccc tcatcggcag ccccggccgg acgttcccac ccgtgcaccc
     8581 gctggatgtg atggccgacg cccgggcact ggaacgtgcc tgctacgagg agagcctgaa
     8641 gagccggcca gggaccgcca gcagctcggg gggctccatt gcgcgcggcg ccccggtcat
     8701 tgtgcctgag ctgggtaagc cgcggcagag ccccctgacc tatgaggacc acggggcacc
     8761 ctttgccggc cacctcccac gaggttcgcc cgtgaccacg cgggagccca cgccgcgcct
     8821 gcaggagggc agcctttcgt ccagcaaggc atcccaggac cgaaagctga cgtcgacgcc
     8881 tcgtgagatc gccaagtccc cgcacagcac cgtgcccgag caccacccac accccatctc
     8941 gccctatgag cacctgcttc ggggcgtgag tggcgtggac ctgtatcgca gccacatccc
     9001 cctggccttc gaccccacct ccataccccg cggcatccct ctggacgcag ccgctgccta
     9061 ctacctgccc cgacacctgg cccccaaccc cacctacccg cacctgtacc caccctacct
     9121 catccgcggc taccccgaca cggcggcgct ggagaaccgg cagaccatca tcaatgacta
     9181 catcacctcg cagcagatgc accacaacgc ggccaccgcc atggcccagc gagctgatat
     9241 gctgaggggc ctctcgcccc gcgagtcctc gctggcactc aactacgctg cgggtccccg
     9301 aggcatcatc gacctgtccc aagtgccaca cctgcctgtg ctcgtgcccc cgacaccagg
     9361 caccccagcc accgccatgg accgccttgc ctacctcccc accgcgcccc agcccttcag
     9421 cagccgccac agcagctccc cactctcccc aggaggtcca acacacttga caaaaccaac
     9481 caccacgtcc tcgtccgagc gggagcgaga ccgggatcga gagcgggacc gggatcggga
     9541 gcgggaaaag tccatcctca cgtccaccac gacggtggag cacgcaccca tctggagacc
     9601 tggtacagag cagagcagcg gcagcagcgg cgggggtggg ggcagcagca gccgccccgc
     9661 ctcccactcc catgcccacc agcactcgcc catctcccct cggacccagg atgccctcca
     9721 gcagagaccc agtgtgcttc acaacacagg catgaagggt atcatcaccg ctgtggagcc
     9781 cagcacgccc acggtcctga ggtccacctc cacctcctca cccgttcgcc cggctgccac
     9841 attcccacct gccacccact gcccactggg cggcaccctc gatggggtct accctaccct
     9901 catggagccc gtcttgctgc ccaaggaggc cccccgggtc gcccggccag agcggccccg
     9961 agcagacacc ggccatgcct tcctcgccaa gcccccagcc cgctccgggc tggagcccgc
    10021 ctcctccccc agcaagggct cggagccccg gcccctagtg cctcctgtct ctggccacgc
    10081 caccatcgcc cgcacccctg cgaagaacct cgcacctcac cacgccagcc cggacccgcc
    10141 ggcgccacct gcctcggcct cggacccgca ccgggaaaag actcaaagta aacccttttc
    10201 catccaggaa ctggaactcc gttctctggg ttaccacggc agcagctaca gccccgaagg
    10261 ggtggagccc gtcagccctg tgagctcacc cagtctgacc cacgacaagg ggctccccaa
    10321 gcacctggaa gagctcgaca agagccacct ggagggggag ctgcggccca agcagccagg
    10381 ccccgtgaag cttggcgggg aggccgccca cctcccacac ctgcggccgc tgcctgagag
    10441 ccagccctcg tccagcccgc tgctccagac cgccccaggg gtcaaaggtc accagcgggt
    10501 ggtcaccctg gcccagcaca tcagtgaggt catcacacag gactacaccc ggcaccaccc
    10561 acagcagctc agcgcacccc tgcccgcccc cctctactcc ttccctgggg ccagctgccc
    10621 cgtcctggac ctccgccgcc cacccagtga cctctacctc ccgcccccgg accatggtgc
    10681 cccggcccgt ggctcccccc acagcgaagg gggcaagagg tctccagagc caaacaagac
    10741 gtcggtcttg ggtggtggtg aggacggtat tgaacctgtg tccccaccgg agggcatgac
    10801 ggagccaggg cactcccgga gtgctgtgta cccgctgctg taccgggatg gggaacagac
    10861 ggagcccagc aggatgggct ccaagtctcc aggcaacacc agccagccgc cagccttctt
    10921 cagcaagctg accgagagca actccgccat ggtcaagtcc aagaagcaag agatcaacaa
    10981 gaagctgaac acccacaacc ggaatgagcc tgaatacaat atcagccagc ctgggacgga
    11041 gatcttcaat atgcccgcca tcaccggaac aggccttatg acctatagaa gccaggcggt
    11101 gcaggaacat gccagcacca acatggggct ggaggccata attagaaagg cactcatggg
    11161 taaatatgac cagtgggaag agtccccgcc gctcagcgcc aatgctttta accctctgaa
    11221 tgccagtgcc agcctgcccg ctgctatgcc cataaccgct gctgacggac ggagtgacca
    11281 cacactcacc tcgccaggtg gcggcgggaa ggccaaggtc tctggcagac ccagcagccg
    11341 aaaagccaag tccccggccc cgggcctggc atctggggac cggccaccct ctgtctcctc
    11401 agtgcactcg gagggagact gcaaccgccg gacgccgctc accaaccgcg tgtgggagga
    11461 caggccctcg tccgcaggtt ccacgccatt cccctacaac cccctgatca tgcggctgca
    11521 ggcgggtgtc atggcttccc cacccccacc gggcctcccc gcgggcagcg ggcccctcgc
    11581 tggcccccac cacgcctggg acgaggagcc caagccactg ctctgctcgc agtacgagac
    11641 actctccgac agcgagtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
    11701 gctttatata tcttgtggaa aggacgacca gctagccaga taatacagaa gacgcgttaa
    11761 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
    11821 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
    11881 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
    11941 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
    12001 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
    12061 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
    12121 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
    12181 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    12241 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    12301 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
    12361 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
    12421 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
    12481 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    12541 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    12601 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    12661 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
    12721 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    12781 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    12841 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
    12901 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    12961 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    13021 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
    13081 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    13141 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    13201 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
    13261 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    13321 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    13381 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    13441 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    13501 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    13561 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    13621 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    13681 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    13741 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    13801 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    13861 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    13921 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    13981 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    14041 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    14101 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    14161 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    14221 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    14281 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    14341 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    14401 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    14461 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    14521 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    14581 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    14641 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    14701 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    14761 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    14821 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    14881 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    14941 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    15001 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    15061 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    15121 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    15181 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    15241 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    15301 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    15361 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    15421 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    15481 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    15541 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    15601 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    15661 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    15721 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    15781 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    15841 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    15901 agctgcaagc
//
