LOCUS       sequence-285300-         785 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2662
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcgtcc ggttcagctg gctccgggtt ctctggctct ggttctgtgc
       61 cgtctggaag ctcagaaagc tgctggtgct gctgaagaac cgggtggtcg tgctgttttc
      121 cgtgctttcc gtcgtgctaa cgctcgttgc ttctggaacg ctcgtctggc tcgtgctgct
      181 tctcgtctgg ctttccaggg ttggctgcgt cgtggtgttc tgctggttcg tgctccgccg
      241 gcttgcctgc aggttctgcg tgacgcttgg cgtcgtcgtg ctctgcgtcc gccgcgtggt
      301 ttccgtatcc gtgctgttgg tgacgttttc ccggttcaga tgaacccgat cacccagtct
      361 cagttcgttc cgctgggtga agttctgtgc tgcgctatct ctgacatgaa caccgctcag
      421 atcgttgtta cccaggaatc tctgctggaa cgtctgatga aacactaccc gggtatcgct
      481 atcccgtctg aagacatcct gtacaccacc ctgggtaccc tgatcaaaga acgtaaaatc
      541 taccacaccg gtgaaggtta cttcatcgtt accccgcaga cctacttcat caccaacacc
      601 accacccagg aaaacaaacg tatgctgccg tctgacgaat ctcgtctgat gccggcttct
      661 atgacctacc tggacaccga atctggtatc tagtaatgaa ctagtccgta acttgaaagt
      721 atttcgattt cttggcttta tatatcttgt ggaaaggacg acaaacctac tctattatcc
      781 tatgt
//
