LOCUS       sequence-285342-        1502 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2933
FEATURES             Location/Qualifiers
     source          1..1502
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1334..1358)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1366..1407
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt tatttgagca gggtcagcag
       61 gccctggagc ttcctgagtg cacaatgcag aaggctgctt actatgaaaa cccaggactg
      121 tttggaggct atggctacag caaaactacg gacacttacg gctacagcac cccccaccag
      181 ccctacccac cccctgctgc tgccagctcc ctggacactg actatccagg ttctgcctgc
      241 tccatccaga gctctgcccc tctgagagcc ccagcccaca aaggagctga actcaatggc
      301 agctgcatgc ggccgggcac tgggaacagc cagggtgggg gtggtggcag ccagcctcct
      361 ggtctgaact cagagcagca gccaccacaa ccccctcctc caccaccgac cctgccccca
      421 tcttcaccca ccaatcctgg aggtggagtg cctgccaaga agcccaaagg tgggcccaat
      481 gcttctagct cctcagccac catcagcaag cagatcttcc cctggatgaa agagtctcga
      541 cagaactcca agcagaagaa cagctgtgcc actgcaggag agagctgcga ggacaagagc
      601 ccgccaggcc cagcatccaa gcgggtacgc acggcataca cgagcgcgca gctggtggaa
      661 ttggaaaagg aattccactt caaccgctac ttgtgccggc cgcgccgcgt ggagatggcc
      721 aacctgctga atctcacgga acgccagatc aagatctggt tccagaaccg gcgcatgaag
      781 tacaagaagg accagaaggc caagggcatc ctgcactcgc cggctagcca gtcccctgag
      841 cgcagcccac cgctcggcgg cgccgctggc cacgtggcct actccggcca gctgccgcca
      901 gtgcccggcc tggcctacga cgcgccctcg ccgcctgctt tcgccaaatc acagcccaat
      961 atgtacggcc tggccgccta cacggcgcca ctcagcagct gcctgccaca acagaagcgc
     1021 tacgcagcgc cggagttcga gccccatccc atggcgagca acggcggcgg cttcgccagc
     1081 gccaacctgc agggcagccc ggtgtacgtg ggcggcaact tcgtcgagtc catggcgccc
     1141 gcgtccgggc ctgtcttcaa cctgggccac ctctcgcacc cgtcgtcggc cagcgtggac
     1201 tacagttgcg ccgcgcagat tccaggcaac caccaccatg gaccttgcga ccctcatccc
     1261 acctacacag atctctcggc ccaccactcg tctcagggac gactgccgga ggctcccaaa
     1321 ctgacgcatc tgtacccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1381 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1441 tcgatttctt ggctttatat atcttgtgga aaggacgaaa tctataacat aaaaagtgtc
     1501 tc
//
