LOCUS       sequence-285423-        3014 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2960
FEATURES             Location/Qualifiers
     source          1..3014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2846..2870)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2878..2919
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcttca ccatgactga actgaaggca
       61 aagggtcccc gggctcccca cgtggcgggc ggcccgccct cccccgaggt cggatcccca
      121 ctgctgtgtc gcccagccgc aggtccgttc ccggggagcc agacctcgga caccttgccc
      181 gaagtttcgg ccatacctat ctccctggac gggctactct tccctcggcc ctgccaggga
      241 caggacccct ccgacgaaaa gacgcaggac cagcagtcgc tgtcggacgt ggagggcgca
      301 tattccagag ctgaagctac aaggggtgct ggaggcagca gttctagtcc cccagaaaag
      361 gacagcggac tgctggacag tgtcttggac actctgttgg cgccctcagg tcccgggcag
      421 agccaaccca gccctcccgc ctgcgaggtc accagctctt ggtgcctgtt tggccccgaa
      481 cttcccgaag atccaccggc tgcccccgcc acccagcggg tgttgtcccc gctcatgagc
      541 cggtccgggt gcaaggttgg agacagctcc gggacggcag ctgcccataa agtgctgccc
      601 cggggcctgt caccagcccg gcagctgctg ctcccggcct ctgagagccc tcactggtcc
      661 ggggccccag tgaagccgtc tccgcaggcc gctgcggtgg aggttgagga ggaggatggc
      721 tctgagtccg aggagtctgc gggtccgctt ctgaagggca aacctcgggc cctgggtggc
      781 gcggcggctg gaggaggagc cgcggctgtc ccgccggggg cggcagcagg aggcgtcgcc
      841 ctggtcccca aggaagattc ccgcttctca gcgcccaggg tcgccctggt ggagcaggac
      901 gcgccgatgg cgcccgggcg ctccccgctg gccaccacgg tgatggattt catccacgtg
      961 cctatcctgc ctctcaatca cgccttattg gcagcccgca ctcggcagct gctggaagac
     1021 gaaagttacg acggcggggc cggggctgcc agcgcctttg ccccgccgcg gagttcaccc
     1081 tgtgcctcgt ccaccccggt cgctgtaggc gacttccccg actgcgcgta cccgcccgac
     1141 gccgagccca aggacgacgc gtaccctctc tatagcgact tccagccgcc cgctctaaag
     1201 ataaaggagg aggaggaagg tgcggaggcc tccgcgcgct ccccgcgttc ctaccttgtg
     1261 gccggtgcca accccgcagc cttcccggat ttcccgttgg ggccaccgcc cccgctgccg
     1321 ccgcgagcga ccccatccag acccggggaa gcggcggtga cggccgcacc cgccagtgcc
     1381 tcagtctcgt ctgcgtcctc ctcggggtcg accctggagt gcatcctgta caaagcggag
     1441 ggcgcgccgc cccagcaggg cccgttcgcg ccgccgccct gcaaggcgcc gggcgcgagc
     1501 ggctgcctgc tcccgcggga cggcctgccc tccacctccg cctctgccgc cgccgccggg
     1561 gcggcccccg cgctctaccc tgcactcggc ctcaacgggc tcccgcagct cggctaccag
     1621 gccgccgtgc tcaaggaggg cctgccgcag gtctacccgc cctatctcaa ctacctgagg
     1681 ccggattcag aagccagcca gagcccacaa tacagcttcg agtcattacc tcagaagatt
     1741 tgtttaatct gtggggatga agcatcaggc tgtcattatg gtgtccttac ctgtgggagc
     1801 tgtaaggtct tctttaagag ggcaatggaa gggcagcaca actacttatg tgctggaaga
     1861 aatgactgca tcgttgataa aatccgcaga aaaaactgcc cagcatgtcg ccttagaaag
     1921 tgctgtcagg ctggcatggt ccttggaggt cgaaaattta aaaagttcaa taaagtcaga
     1981 gttgtgagag cactggatgc tgttgctctc ccacagccag tgggcgttcc aaatgaaagc
     2041 caagccctaa gccagagatt cactttttca ccaggtcaag acatacagtt gattccacca
     2101 ctgatcaacc tgttaatgag cattgaacca gatgtgatct atgcaggaca tgacaacaca
     2161 aaacctgaca cctccagttc tttgctgaca agtcttaatc aactaggcga gaggcaactt
     2221 ctttcagtag tcaagtggtc taaatcattg ccaggttttc gaaacttaca tattgatgac
     2281 cagataactc tcattcagta ttcttggatg agcttaatgg tgtttggtct aggatggaga
     2341 tcctacaaac acgtcagtgg gcagatgctg tattttgcac ctgatctaat actaaatgaa
     2401 cagcggatga aagaatcatc attctattca ttatgcctta ccatgtggca gatcccacag
     2461 gagtttgtca agcttcaagt tagccaagaa gagttcctct gtatgaaagt attgttactt
     2521 cttaatacaa ttcctttgga agggctacga agtcaaaccc agtttgagga gatgaggtca
     2581 agctacatta gagagctcat caaggcaatt ggtttgaggc aaaaaggagt tgtgtcgagc
     2641 tcacagcgtt tctatcaact tacaaaactt cttgataact tgcatgatct tgtcaaacaa
     2701 cttcatctgt actgcttgaa tacatttatc cagtcccggg cactgagtgt tgaatttcca
     2761 gaaatgatgt ctgaagttat tgctgcacaa ttacccaaga tattggcagg gatggtgaaa
     2821 ccccttctct ttcataaaaa gtgagaccca gctttcttgt acaaagtggt tgatatcggt
     2881 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     2941 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga cccatccgaa
     3001 agcaactaac cctt
//
