LOCUS       sequence-285483-        1502 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2980
FEATURES             Location/Qualifiers
     source          1..1502
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1334..1358)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1366..1407
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgcccaaccc caggcctggc
       61 aagccctcgg ccccttcctt ggcccttggc ccatccccag gagcctcgcc cagctggagg
      121 gctgcaccca aagcctcaga cctgctgggg gcccggggcc cagggggaac cttccagggc
      181 cgagatcttc gaggcggggc ccatgcctcc tcttcttcct tgaaccccat gccaccatcg
      241 cagctgcagc tgcccacact gcccctagtc atggtggcac cctccggggc acggctgggc
      301 cccttgcccc acttacaggc actcctccag gacaggccac atttcatgca ccagctctca
      361 acggtggatg cccacgcccg gacccctgtg ctgcaggtgc accccctgga gagcccagcc
      421 atgatcagcc tcacaccacc caccaccgcc actggggtct tctccctcaa ggcccggcct
      481 ggcctcccac ctgggatcaa cgtggccagc ctggaatggg tgtccaggga gccggcactg
      541 ctctgcacct tcccaaatcc cagtgcaccc aggaaggaca gcaccctttc ggctgtgccc
      601 cagagctcct acccactgct ggcaaatggt gtctgcaagt ggcccggatg tgagaaggtc
      661 ttcgaagagc cagaggactt cctcaagcac tgccaggcgg accatcttct ggatgagaag
      721 ggcagggcac aatgtctcct ccagagagag atggtacagt ctctggagca gcagctggtg
      781 ctggagaagg agaagctgag tgccatgcag gcccacctgg ctgggaaaat ggcactgacc
      841 aaggcttcat ctgtggcatc atccgacaag ggctcctgct gcatcgtagc tgctggcagc
      901 caaggccctg tcgtcccagc ctggtctggc ccccgggagg cccctgacag cctgtttgct
      961 gtccggaggc acctgtgggg tagccatgga aacagcacat tcccagagtt cctccacaac
     1021 atggactact tcaagttcca caacatgcga ccccctttca cctacgccac gctcatccgc
     1081 tgggccatcc tggaggctcc agagaagcag cggacactca atgagatcta ccactggttc
     1141 acacgcatgt ttgccttctt cagaaaccat cctgccacct ggaagaacgc catccgccac
     1201 aacctgagtc tgcacaagtg ctttgtgcgg gtggagagcg agaagggggc tgtgtggacc
     1261 gtggatgagc tggagttccg caagaaacgg agccagaggc ccagcaggtg ttccaaccct
     1321 acacctggcc ccttgccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
     1381 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
     1441 tcgatttctt ggctttatat atcttgtgga aaggacgata gcgctggata tgccgggcac
     1501 ca
//
