LOCUS       sequence-285507-        1097 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2988
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(929..953)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             961..1002
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga tgtcttatct taagcaaccg
       61 ccttacgcag tcaatgggct gagtctgacc acttcgggta tggacttgct gcacccctcc
      121 gtgggctacc cggggccctg ggcttcttgt cccgcagcca ccccccggaa acagcgccgg
      181 gagaggacga cgttcactcg ggcgcagcta gatgtgctgg aagcactgtt tgccaagacc
      241 cggtacccag acatcttcat gcgagaggag gtggcactga aaatcaactt gcccgagtcg
      301 agggtgcagg tatggtttaa gaatcgaaga gctaagtgcc gccaacaaca gcaacaacag
      361 cagaatggag gtcaaaacaa agtgagacct gccaaaaaga agacatctcc agctcgggaa
      421 gtgagttcag agagtggaac aagtggccaa ttcactcccc cctctagcac ctcagtcccg
      481 accattgcca gcagcagtgc tcctgtgtct atctggagcc cagcttccat ctccccactg
      541 tcagatccct tgtccacctc ctcttcctgc atgcagaggt cctatcccat gacctatact
      601 caggcttcag gttatagtca aggatatgct ggctcaactt cctactttgg gggcatggac
      661 tgtggatcat atttgacccc tatgcatcac cagcttcccg gaccaggggc cacactcagt
      721 cccatgggta ccaatgcagt caccagccat ctcaatcagt ccccagcttc tctttccacc
      781 cagggatatg gagcttcaag cttgggtttt aactcaacca ctgattgctt ggattataag
      841 gaccaaactg cctcctggaa gcttaacttc aatgctgact gcttggatta taaagatcag
      901 acatcctcgt ggaaattcca ggttttgtgc ccaactttct tgtacaaagt ggttgatatc
      961 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1021 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatccgcac
     1081 gctcgaaaaa acgtcgt
//
