LOCUS       sequence-285537-        1646 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2998
FEATURES             Location/Qualifiers
     source          1..1646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1478..1502)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1510..1551
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg aggtggcgcc ggagcagccg
       61 cgctggatgg cgcacccggc cgtgctgaat gcgcagcacc ccgactcaca ccacccgggc
      121 ctggcgcaca actacatgga acccgcgcag ctgctgcctc cagacgaggt ggacgtcttc
      181 ttcaatcacc tcgactcgca gggcaacccc tactatgcca accccgctca cgcgcgggcg
      241 cgcgtctcct acagccccgc gcacgcccgc ctgaccggag gccagatgtg ccgcccacac
      301 ttgttgcaca gcccgggttt gccctggctg gacgggggca aagcagccct ctctgccgct
      361 gcggcccacc accacaaccc ctggaccgtg agccccttct ccaagacgcc actgcacccc
      421 tcagctgctg gaggccctgg aggcccactc tctgtgtacc caggggctgg gggtgggagc
      481 gggggaggca gcgggagctc agtggcctcc ctcaccccta cagcagccca ctctggctcc
      541 caccttttcg gcttcccacc cacgccaccc aaagaagtgt ctcctgaccc tagcaccacg
      601 ggggctgcgt ctccagcctc atcttccgcg gggggtagtg cagcccgagg agaggacaag
      661 gacggcgtca agtaccaggt gtcactgacg gagagcatga agatggaaag tggcagtccc
      721 ctgcgcccag gcctagctac tatgggcacc cagcctgcta cacaccaccc catccccacc
      781 tacccctcct atgtgccggc ggctgcccac gactacagca gcggactctt ccaccccgga
      841 ggcttcctgg ggggaccggc ctccagcttc acccctaagc agcgcagcaa ggctcgttcc
      901 tgttcagaag gccgggagtg tgtcaactgt ggggccacag ccacccctct ctggcggcgg
      961 gacggcaccg gccactacct gtgcaatgcc tgtggcctct accacaagat gaatgggcag
     1021 aaccgaccac tcatcaagcc caagcgaaga ctgtcggccg ccagaagagc cggcacctgt
     1081 tgtgcaaatt gtcagacgac aaccaccacc ttatggcgcc gaaacgccaa cggggaccct
     1141 gtctgcaacg cctgtggcct ctactacaag ctgcacaatg ttaacaggcc actgaccatg
     1201 aagaaggaag ggatccagac tcggaaccgg aagatgtcca acaagtccaa gaagagcaag
     1261 aaaggggcgg agtgcttcga ggagctgtca aagtgcatgc aggagaagtc atcccccttc
     1321 agtgcagctg ccctggctgg acacatggca cctgtgggcc acctcccgcc cttcagccac
     1381 tccggacaca tcctgcccac tccgacgccc atccacccct cctccagcct ctccttcggc
     1441 cacccccacc cgtccagcat ggtgaccgcc atgggctacc caactttctt gtacaaagtg
     1501 gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
     1561 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1621 gacggtatcc ggcttgtaac tcaatc
//
