LOCUS       sequence-285594-         827 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3017
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(659..683)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             691..732
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt acctccacac agagggcttc
       61 tctgggccct ctccaggtga cggggccatg ggctatggct atgagaaacc tctgcgacca
      121 ttcccagatg atgtctgcgt tgtccctgag aaatttgaag gagacatcaa gcaggaaggg
      181 gtcggtgcat ttcgagaggg gccgccctac cagcgccggg gtgccctgca gctgtggcaa
      241 tttctggtgg ccttgctgga tgacccaaca aatgcccatt tcattgcctg gacgggccgg
      301 ggaatggagt tcaagctcat tgagcctgag gaggtcgcca ggctctgggg catccagaag
      361 aaccggccag ccatgaatta cgacaagctg agccgctcgc tccgatacta ttatgagaaa
      421 ggcatcatgc agaaggtggc tggtgagcgt tacgtgtaca agtttgtgtg tgagcccgag
      481 gccctcttct ctttggcctt cccggacaat cagcgtccag ctctcaaggc tgagtttgac
      541 cggcctgtca gtgaggagga cacagtccct ttgtcccact tggatgagag ccccgcctac
      601 ctcccagagc tggctggccc cgcccagcca tttggcccca agggtggcta ctcttactac
      661 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
      721 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
      781 tatatatctt gtggaaagga cgaatagcct catcacgttt cctccga
//
