LOCUS       sequence-285702-        1565 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3053
FEATURES             Location/Qualifiers
     source          1..1565
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1397..1421)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1429..1470
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg gtgtgaaaac atttactcat
       61 agctcctctt cccacagcca ggaaatgctt ggaaagctaa atatgctgcg aaatgatgga
      121 catttttgtg atatcactat tcgtgtccag gacaaaatct tccgggcaca taaggtggta
      181 ctagcagctt gcagtgattt ctttcgcacc aaacttgtag gccaagccga ggatgagaac
      241 aagaatgtgt tggatctgca tcatgttaca gtgactggct ttatacctct tttagaatat
      301 gcttacacag ccactctatc aattaacaca gaaaatatta ttgatgttct agcagcagcc
      361 agctatatgc aaatgttcag tgttgccagc acctgctcag agttcatgaa atcaagcatt
      421 ttatggaata cacccaacag ccaacctgaa aagggtctag atgctggaca agaaaataat
      481 tctaactgca attttacttc tcgagatggg agcatttctc ccgtgtcctc agagtgcagt
      541 gtggtagaaa gaaccattcc tgtctgccga gaatcccgga gaaagcgcaa aagctacatt
      601 gttatgtctc ctgaaagtcc tgtaaagtgt ggcacacaaa caagctcacc ccaggtattg
      661 aattcttcag cttcctactc agaaaataga aaccaaccag ttgactcttc cttagctttt
      721 ccttggactt ttccttttgg aattgatcga aggattcagc ctgagaaagt taagcaagca
      781 gaaaataccc ggactttaga attacctggc ccatctgaga ccggtagaag aatggctgat
      841 tatgtgactt gtgagagcac aaaaactacc ttgcctttag gtaccgaaga agatgtccgg
      901 gtcaaagtag aaagattaag tgatgaggag gtccatgagg aagtgtccca gcctgtcagt
      961 gcatctcaga gttcgctgag tgatcagcag acagttccag gaagtgaaca agtccaagag
     1021 gaccttctga ttagtccaca gtcttcctct ataggctcag tagatgaagg cgtttctgag
     1081 ggcttgccta cacttcaaag cacgtctagc actaatgctc ctccggatga tgatgatcga
     1141 ttggaaaatg ttcagtatcc ctaccaactc tacattgctc cttccaccag cagtacagag
     1201 cgaccaagtc caaatggtcc cgacagacct tttcagtgtc caacctgcgg ggtgcgattc
     1261 acccgtattc agaacctaaa gcagcacatg ctcatccact caggaattaa accatttcag
     1321 tgtgaccgct gtgggaaaaa gttcaccagg gcttactcgc taaagatgca tcgcctaaag
     1381 catgaagtga tttcctaccc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1441 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1501 atttcgattt cttggcttta tatatcttgt ggaaaggacg actctatcca attctcgaac
     1561 tcacc
//
