LOCUS       sequence-285744-        1931 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3067
FEATURES             Location/Qualifiers
     source          1..1931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1763..1787)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1795..1836
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg accccgagga cgaaggggta
       61 gcgggagtga tgtctgtggg gccgccggcg gcccggcttc aggaaccagt gaccttccgg
      121 gatgtggctg tggactttac ccaggaggaa tgggggcagc tggaccctac ccagaggatc
      181 ctctaccgtg acgtgatgct ggagaccttt ggtcacctgc tctccatagg tcctgagctt
      241 ccgaagcctg aagtcatctc ccagctggag caagggaccg agctatgggt ggctgagaga
      301 ggaaccaccc agggctgcca tccagcctgg gagcctcgat ctgaaagcca agcatcacgc
      361 aaggaagagg gcctgcctga agaggagcca tcccatgtca cgggaaggga aggattcccg
      421 acagatgctc cttatcccac cacgttaggg aaagacaggg agtgtcagag ccagagtctg
      481 gcactcaagg agcagaataa cttgaagcag ttggaatttg gcctcaagga agcaccagtt
      541 caagatcaag gctacaaaac tctcagactc agggaaaact gcgtcctgag ttcaagccca
      601 aatccattcc cagagatctc tagaggggag tatttgtata cttacgactc acagattaca
      661 gactcagaac ataactccag cttagtcagt cagcagacag gctccccagg aaaacagccc
      721 ggtgaaaaca gtgactgtca cagagattcc agtcaggcca ttccaattac ggaactcaca
      781 aaaagccagg tgcaggacaa accctacaaa tgtactgact gtgggaagtc gtttaaccat
      841 aacgcacacc tcaccgtgca caagaggatt catacgggag aaagacctta tatgtgcaag
      901 gagtgtggga aagccttcag ccagaactcc tccctcgtcc agcatgagcg catccacact
      961 ggagacaagc cctacaagtg tgccgaatgt gggaagtctt tctgccatag tacacacctt
     1021 accgtccatc ggaggattca cactggggag aagccctatg agtgtcagga ctgtgggagg
     1081 gccttcaacc agaactcctc cctggggcgg cacaagagga cacacactgg ggagaagcca
     1141 tacacctgca gtgtgtgtgg gaaatccttc tctcggacca cttgcctttt cctgcacctg
     1201 agaactcaca ccgaggagag gccctacgag tgtaaccact gcgggaaggg cttcaggcac
     1261 agctcatccc tggcccagca ccagcggaag cacgcggggg agaagccctt tgagtgccgc
     1321 cagaggctga tctttgagca gacgccagct ctcacaaagc atgaatggac agaagccctg
     1381 ggctgtgacc cacctttgag tcaagatgag aggactcacc gaagcgacag acccttcaaa
     1441 tgtaatcagt gtgggaagtg tttcattcag agctctcacc tcatccggca ccagataact
     1501 cacaccagag aggagcagcc ccatgggcga agccggcggc gtgaacagtc ctcgagcagg
     1561 aactcacacc tggttcagca tcaacacccg aactccagaa agagctctgc aggcggagca
     1621 aaggcagggc agccggaaag cagagccctg gctttgtttg acatccaaaa aatcatgcaa
     1681 gagaaaaacc ctgtgcacgt tattggggtg gaagagcctt ctgtgggtgc ttccatgtta
     1741 tttgacatca gagaatccac atacccaact ttcttgtaca aagtggttga tatcggtaag
     1801 cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag tccgtaactt
     1861 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagga ctcacaaccg
     1921 attttttaca c
//
