LOCUS       sequence-285825-        1790 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3094
FEATURES             Location/Qualifiers
     source          1..1790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1622..1646)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1654..1695
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgtaccaacc agaagacgac
       61 aacaacagtg acgtgaccag tgacgacgac atgacccgga acagaagaga gtcctcacca
      121 cctcactcag tccattcttt cagtggtgac cgggactggg accggagggg cagaagcaga
      181 gacatggagc cacgagaccg ctggtcccac accaggaacc caagaagcag gatgcctccg
      241 cgggatcttt cccttcctgt ggtggcgaaa acaagctttg aaatggacag agaggacgac
      301 agggactcca gggcttatga gtcccgatct caggatgctg aatcatacca aaatgtggtg
      361 gacctcgctg aggacaggaa acctcacaac acaatccagg acaacatgga aaactacagg
      421 aagctgctct ccctcggttt ccttgctcag gactctgtcc ctgcagaaaa gaggaacaca
      481 gagatgttag acaatctgcc atctgctggg tcccagttcc cggacttcaa acacttagga
      541 acatttctgg tgtttgagga gttggtgacc ttcgaggatg tgcttgtgga cttcagccca
      601 gaggaactta gctcccttag tgctgctcag agaaacctct acagggaggt gatgctggag
      661 aattaccgga acctggtctc cctggggcac cagttctcta aacctgacat tatctcacgc
      721 ctggaagagg aggaatcata tgcaatggag acagacagca gacatacagt gatttgtcaa
      781 ggagagtctc atgatgatcc attggaacca caccagggca accaagagaa acttttgact
      841 cctataacaa tgaatgaccc caagaccctc actccggaaa gaagctatgg cagtgatgaa
      901 tttgagagaa gctctaatct tagtaaacaa tcaaaggatc ctctaggaaa ggatccccag
      961 gaaggcactg ctcctggaat atgtacgagt ccccagtcag catcccaaga gaacaaacac
     1021 aacagatgtg aattttgcaa acgaaccttt agtacgcaag tagcccttag gagacacgaa
     1081 cggatccata ctgggaagaa accctatgaa tgtaaacagt gtgctgaagc cttctatctc
     1141 atgccacacc tcaacagaca tcagaagacc cattctggta ggaagacttc tggctgcaat
     1201 gaaggtagaa agccttccgt ccagtgtgcg aatctctgtg aacgtgtaag aattcacagt
     1261 caggaggact actttgaatg ttttcagtgc ggcaaagctt ttctccagaa tgtgcatctt
     1321 cttcaacatc tcaaagccca tgaggcagca agagtccttc ctcctgggtt gtcccacagc
     1381 aagacatact taattcgtta tcagcggaaa catgactacg ttggagagag agcctgccag
     1441 tgttgtgact gtggcagagt cttcagtcgg aattcatatc tcattcagca ttatagaact
     1501 cacactcaag agaggcctta ccagtgtcag ctatgtggga aatgtttcgg ccgaccctca
     1561 tacctcactc aacattatca actccattct caagagaaaa ctgttgagtg cgatcactgt
     1621 ttgccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1681 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1741 ctttatatat cttgtggaaa ggacgatagg actactaaac cggaaatgat
//
