LOCUS       sequence-285828-        1883 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3095
FEATURES             Location/Qualifiers
     source          1..1883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1715..1739)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1747..1788
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgcaccgcgg cgggcagcct
       61 ttgaaaaagc ggcgcggctc gttcaagatg gcggagctcg accagttgcc tgacgagagc
      121 tcttcagcaa aagcccttgt cagtttaaaa gaaggaagct tatctaacac gtggaatgaa
      181 aagtacagtt ctttacagaa aacacctgtt tggaaaggca ggaatacaag ctctgctgtg
      241 gaaatgcctt tcagaaattc aaaacgaagt cgactttttt ctgatgaaga tgataggcaa
      301 ataaatacaa ggtcacctaa aagaaaccag agggttgcaa tggttccaca gaaatttaca
      361 gcaacaatgt caacaccaga taagaaagct tcacagaaga ttggttttcg attacgtaat
      421 ctgctcaagc ttcctaaagc acataaatgg tgtatatacg agtggttcta ttcaaatata
      481 gataaaccac tttttgaagg tgataatgac ttctgtgtat gtctaaagga atcttttcct
      541 aatttgaaaa caagaaagtt aacaagagta gaatggggaa aaattcggcg gcttatggga
      601 aaaccacgga gatgttcttc tgcatttttt gaggaagaga gatcagcatt aaaacagaaa
      661 cggcagaaaa taaggctctt acaacaaagg aaagttgcag atgtttcaca attcaaagat
      721 ctcccagatg aaattccttt gcctctggtt attggaacga aagttacagc acgattacgt
      781 ggtgttcatg atggtttgtt cactggacaa atagatgctg tggatactct taatgctact
      841 tatagagtaa cttttgatag gacagggctt ggaacccata ccatccctga ctatgaagtt
      901 ctcagtaatg aacctcatga gacaatgcca attgctgcct ttggacaaaa acagcggcct
      961 tctcgatttt ttatgacccc accacggtta cattatactc ctcctctcca gtcaccaatt
     1021 atagataatg atcctttatt aggacagtcg ccgtggagaa gtaaaatttc tggctctgac
     1081 actgaaacat taggtggttt tccagtagaa tttcttatcc aagtgaccag attatcaaaa
     1141 attctcatga ttaaaaagga acatatcaag aaattaaggg aaatgaacac agaagcagaa
     1201 aaattgaaat catattccat gcccatcagc attgaatttc agcggagata tgcaacaatt
     1261 gttctggagc ttgaacagct gaacaaggac ctaaacaaag ttttgcataa agttcaacag
     1321 tattgctatg agcttgctcc agaccagggg ctccagcctg cagatcagcc aacagatatg
     1381 agacgcaggt gtgaggaaga agcacaggaa attgttcggc atgcaaattc ctcaacagga
     1441 cagccctgcg ttgaaaatga aaatctgaca gacttaattt ccaggcttac agctattttg
     1501 ttacaaatta agtgtctagc agaaggagga gacctgaatt cctttgaatt caaatcactt
     1561 acagactcat taaatgatat caagagtaca atagacgctt ctaatatcag ttgctttcag
     1621 aataatgtag aaatccatgt tgcacatatt cagagtggcc tgagccagat gggaaactta
     1681 catgcctttg cagcaaataa caccaacaga gacttgccaa ctttcttgta caaagtggtt
     1741 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1801 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     1861 gccgcgagtg ttgagatgac aat
//
