LOCUS       sequence-286059-        2747 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3172
FEATURES             Location/Qualifiers
     source          1..2747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2579..2603)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2611..2652
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt ctctgtgggg tctggtctcc
       61 aagatgcccc cagaaaaagt gcagcggctc tatgtcgact ttccccaaca cctgcggcat
      121 cttctgggtg actggctgga gagccagccc tgggagttcc tggtcggctc cgacgccttc
      181 tgctgcaact tggctagtgc cctactttca gacactgtcc agcaccttca ggcctcggtg
      241 ggagagcagg gggaggggag caccatcttg caacacatca gcacccttga gagcatatat
      301 cagagggacc ccctgaagct ggtggccact ttcagacaaa tacttcaagg agagaaaaaa
      361 gctgttatgg aacagttccg ccacttgcca atgcctttcc actggaagca ggaagaactc
      421 aagtttaaga caggcttgcg gaggctgcag caccgagtag gggagatcca ccttctccga
      481 gaagccctgc agaagggggc tgaggctggc caagtgtctc tgcacagctt gatagaaact
      541 cctgctaatg ggactgggcc aagtgaggcc ctggccatgc tactgcagga gaccactgga
      601 gagctagagg cagccaaagc cctagtgctg aagaggatcc agatttggaa acggcagcag
      661 cagctggcag ggaatggcgc accgtttgag gagagcctgg ccccactcca ggagaggtgt
      721 gaaagcctgg tggacattta ttcccagcta cagcaggagg taggggcggc tggtggggag
      781 cttgagccca agacccgggc atcgctgact ggccggctgg atgaagtcct gagaaccctc
      841 gtcaccagtt gcttcctggt ggagaagcag cccccccagg tactgaagac tcagaccaag
      901 ttccaggctg gagttcgatt cctgttgggc ttgaggttcc tgggggcccc agccaagcct
      961 ccgctggtca gggccgacat ggtgacagag aagcaggcgc gggagctgag tgtgcctcag
     1021 ggtcctgggg ctggagcaga aagcactgga gaaatcatca acaacactgt gcccttggag
     1081 aacagcattc ctgggaactg ctgctctgcc ctgttcaaga acctgcttct caagaagatc
     1141 aagcggtgtg agcggaaggg cactgagtct gtcacagagg agaagtgcgc tgtgctcttc
     1201 tctgccagct tcacacttgg ccccggcaaa ctccccatcc agctccaggc cctgtctctg
     1261 cccctggtgg tcatcgtcca tggcaaccaa gacaacaatg ccaaagccac tatcctgtgg
     1321 gacaatgcct tctctgagat ggaccgcgtg ccctttgtgg tggctgagcg ggtgccctgg
     1381 gagaagatgt gtgaaactct gaacctgaag ttcatggctg aggtggggac caaccggggg
     1441 ctgctcccag agcacttcct cttcctggcc cagaagatct tcaatgacaa cagcctcagt
     1501 atggaggcct tccagcaccg ttctgtgtcc tggtcgcagt tcaacaagga gatcctgctg
     1561 ggccgtggct tcaccttttg gcagtggttt gatggtgtcc tggacctcac caaacgctgt
     1621 ctccggagct actggtctga ccggctgatc attggcttca tcagcaaaca gtacgttact
     1681 agccttcttc tcaatgagcc cgacggaacc tttctcctcc gcttcagcga ctcagagatt
     1741 gggggcatca ccattgccca tgtcatccgg ggccaggatg gctctccaca gatagagaac
     1801 atccagccat tctctgccaa agacctgtcc attcgctcac tgggggaccg aatccgggat
     1861 cttgctcagc tcaaaaatct ctatcccaag aagcccaagg atgaggcttt ccggagccac
     1921 tacaagcctg aacagatggg taaggatggc aggggttatg tcccagctac catcaagatg
     1981 accgtggaaa gggaccaacc acttcctacc ccagagctcc agatgcctac catggtgcct
     2041 tcttatgacc ttggaatggc ccctgattcc tccatgagca tgcagcttgg cccagatatg
     2101 gtgccccagg tgtacccacc acactctcac tccatccccc cgtatcaagg cctctcccca
     2161 gaagaatcag tcaacgtgtt gtcagccttc caggagcctc acctgcagat gccccccagc
     2221 ctgggccaga tgagcctgcc ctttgaccag cctcaccccc agggcctgct gccgtgccag
     2281 cctcaggagc atgctgtgtc cagccctgac cccctgctct gctcagatgt gaccatggtg
     2341 gaagacagct gcctgagcca gccagtgaca gcgtttcctc agggcacttg gattggtgaa
     2401 gacatattcc ctcctctgct gcctcccact gaacaggacc tcactaagct tctcctggag
     2461 gggcaagggg agtcgggggg agggtccttg ggggcacagc ccctcctgca gccctcccac
     2521 tatgggcaat ctgggatctc aatgtcccac atggacctaa gggccaaccc cagttggtgc
     2581 ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     2641 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2701 tatatatctt gtggaaagga cgatcttaat ttaggttact gtgttaa
//
