LOCUS       sequence-286140-        2357 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3199
FEATURES             Location/Qualifiers
     source          1..2357
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2189..2213)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2221..2262
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tgccaagcac cagctttcca
       61 gtcccttcca agtttccact tggccctgcg gctgcggtct tcgggagagg agaaactttg
      121 gggcccgcgc cgcgcgccgg cggcaccatg aagtcagcgg aggaagaaca ctatggctat
      181 gcatcctcca acgtcagccc cgccctgccg ctccccacgg cgcactccac cctgccggcc
      241 ccgtgccaca accttcagac ctccacaccg ggcatcatcc cgccggcgga tcacccctcg
      301 gggtacggag cagctttgga cggtgggccc gcgggctact tcctctcctc cggccacacc
      361 aggcctgatg gggcccctgc cctggagagt cctcgcatcg agataacctc gtgcttgggc
      421 ctgtaccaca acaataacca gtttttccac gatgtggagg tggaagacgt cctccctagc
      481 tccaaacggt ccccctccac ggccacgctg agtctgccca gcctggaggc ctacagagac
      541 ccctcgtgcc tgagcccggc cagcagcctg tcctcccgga gctgcaactc agaggcctcc
      601 tcctacgagt ccaactactc gtacccgtac gcgtcccccc agacgtcgcc atggcagtct
      661 ccctgcgtgt ctcccaagac cacggacccc gaggagggct ttccccgcgg gctgggggcc
      721 tgcacactgc tgggttcccc gcggcactcc ccctccacct cgccccgcgc cagcgtcact
      781 gaggagagct ggctgggtgc ccgctcctcc agacccgcgt ccccgtgcaa caagaggaag
      841 tacagcctca acggccggca gccgccctac tcaccccacc actcgcccac gccgtccccg
      901 cacggctccc cgcgggtcag cgtgaccgac gactcgtggt tgggcaacac cacccagtac
      961 accagctcgg ccatcgtggc cgccatcaac gcgctgacca ccgacagcag cctggacctg
     1021 ggagatggcg tccctgtcaa gtcccgcaag accaccctgg agcagccgcc ctcagtggcg
     1081 ctcaaggtgg agcccgtcgg ggaggacctg ggcagccccc cgcccccggc cgacttcgcg
     1141 cccgaagact actcctcttt ccagcacatc aggaagggcg gcttctgcga ccagtacctg
     1201 gcggtgccgc agcaccccta ccagtgggcg aagcccaagc ccctgtcccc tacgtcctac
     1261 atgagcccga ccctgcccgc cctggactgg cagctgccgt cccactcagg cccgtatgag
     1321 cttcggattg aggtgcagcc caagtcccac caccgagccc actacgagac ggagggcagc
     1381 cggggggccg tgaaggcgtc ggccggagga caccccatcg tgcagctgca tggctacttg
     1441 gagaatgagc cgctgatgct gcagcttttc attgggacgg cggacgaccg cctgctgcgc
     1501 ccgcacgcct tctaccaggt gcaccgcatc acagggaaga ccgtgtccac caccagccac
     1561 gaggccatcc tctccaacac caaagtcctg gagatcccac tcctgccgga gaacagcatg
     1621 cgagccgtca ttgactgtgc cggaatcctg aaactcagaa actccgacat tgaacttcgg
     1681 aaaggagaga cggacatcgg gaggaagaac acacgggtac ggctggtgtt ccgcgttcac
     1741 gtcccgcaac ccagcggccg cacgctgtcc ctgcaggtgg cctccaaccc catcgaatgc
     1801 tcccagcgct cagctcagga gctgcctctg gtggagaagc agagcacgga cagctatccg
     1861 gtcgtgggcg ggaagaagat ggtcctgtct ggccacaact tcctgcagga ctccaaggtc
     1921 attttcgtgg agaaagcccc agatggccac catgtctggg agatggaagc gaaaactgac
     1981 cgggacctgt gcaagccgaa ttctctggtg gttgagatcc cgccgtttcg gaatcagagg
     2041 ataaccagcc ccgttcacgt cagtttctac gtctgcaacg ggaagagaaa gcgaagccag
     2101 taccagcgtt tcacctacct tcccgccaac ggtaacgcca tctttctaac cgtaagccgt
     2161 gaacatgagc gcgtggggtg ctttttcttg ccaactttct tgtacaaagt ggttgatatc
     2221 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     2281 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgagccgaag
     2341 gagtgctttt tacagcg
//
