LOCUS       sequence-286194-        2603 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3217
FEATURES             Location/Qualifiers
     source          1..2603
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2435..2459)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2467..2508
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc catctcagaa ctatgacctt
       61 ccccagaaga agcaggagaa aatgaccaag tttcaggagg ctgtgacatt caaggatgtg
      121 gctgtggtct tctccaggga ggaactgcga ctgctcgatc ttacccagag gaagctgtac
      181 cgagatgtca tggtggagaa cttcaagaac ctggttgcag tggggcatct tcccttccaa
      241 ccagatatgg tatcccaatt ggaagcagaa gaaaagcttt ggatgatgga aacagaaacc
      301 caaagaagca gcaagcatca aaataagatg gaaacactcc aaaaatttgc attaaaatac
      361 ctttcaaatc aagagctgtc ctgctggcaa atctggaaac aggttgcaag tgaattaacc
      421 aggtgtcttc aggggaagag ttcccagtta ttacaaggtg actctattca ggtttctgaa
      481 aatgagaaca atataatgaa ccctaaagga gatagctcta tttatattga aaatcaagag
      541 tttccatttt ggagaaccca gcattcttgc gggaatacat atctgagtga gtcacagatt
      601 cagagtagag gtaagcaaat tgatgtgaaa aataacctgc aaatacatga agacttcatg
      661 aagaaatcac catttcatga gcatattaaa actgacacag aaccaaaacc ctgcaaaggt
      721 aatgaatatg gcaaaatcat tagtgatggc tccaaccaga aattaccctt aggagagaaa
      781 ccccatccat gtggtgagtg tggaaggggc ttcagttata gcccaaggct tccccttcat
      841 ccgaatgttc acacaggaga aaaatgcttc agtcaaagct cacatctgcg aactcatcag
      901 agaattcacc caggagagaa actcaataga tgtcatgaat ctggtgattg cttcaataag
      961 agctcttttc attcttatca atctaatcat acaggagaga agtcttatag atgcgacagt
     1021 tgcggcaagg gattcagtag cagcacgggt cttatcattc attacagaac tcatactgga
     1081 gagaaaccct ataaatgcga ggaatgtggt aaatgcttta gtcaaagttc aaattttcag
     1141 tgccatcaga gagtccacac tgaagaaaaa ccatacaaat gcgaagagtg tggtaagggc
     1201 ttcggttgga gtgttaatct ccgtgttcac cagagggtcc acaggggtga gaagccctat
     1261 aaatgtgagg aatgtggtaa gggcttcact caggctgcac attttcacat ccatcagaga
     1321 gtccacactg gagagaaacc ctacaagtgt gatgtgtgtg gtaagggctt cagccacaat
     1381 tcaccattaa tatgccatcg gagagtccac acaggagaga agccatacaa gtgtgaggcg
     1441 tgtgggaaag gctttacccg taatacagat ctgcatattc atttcagagt tcacacggga
     1501 gagaaaccct ataaatgtaa ggagtgtggt aagggcttca gtcaggcttc aaatcttcaa
     1561 gtccatcaga atgtccacac tggggagaaa cgattcaagt gtgaaacgtg tgggaagggc
     1621 ttcagtcagt cctcaaagct tcaaacccat cagcgagtcc acactggaga gaaaccatat
     1681 agatgtgatg tgtgtggtaa ggacttcagt tatagttcaa atcttaaact acaccaagta
     1741 attcacactg gagaaaaacc atataaatgt gaggaatgtg ggaagggctt cagttggaga
     1801 tcaaatcttc atgcacatca aagagttcac tcaggagaaa aaccctataa atgtgagcag
     1861 tgtgataaga gcttcagtca ggccatagat tttcgggtac atcagagagt ccatactgga
     1921 gagaagccat acaaatgtgg tgtctgtggt aagggcttca gtcagtcctc tggtcttcaa
     1981 tcccatcaga gagtccacac gggggaaaag ccatacaaat gtgatgtgtg tggaaagggc
     2041 tttagataca gttcgcagtt tatataccat cagagaggcc acactggaga aaaaccttac
     2101 aaatgtgaag agtgtgggaa aggctttggt aggagcttga atcttcgcca tcatcagagg
     2161 gtccacacgg gagagaaacc ccatatatgt gaggagtgtg gtaaggcctt cagtctcccc
     2221 tcaaatcttc gagtccacct gggtgttcac accagggaaa aactctttaa atgtgaagag
     2281 tgtggtaaag gcttcagtca gagtgcacgt cttgaagccc atcagagagt ccacactgga
     2341 gaaaaaccat acaaatgtga catatgtgat aaggacttcc gtcaccgttc acgtcttaca
     2401 tatcatcaga aagtccatac tggtaaaaag ctttacccaa ctttcttgta caaagtggtt
     2461 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     2521 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
     2581 tgtcaatact acggaaattt aaa
//
