LOCUS       sequence-286233-        1469 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3230
FEATURES             Location/Qualifiers
     source          1..1469
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1301..1325)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1333..1374
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cggagagctg gctgcgcctc
       61 tcgggagccg ggccggcgga ggaggccggg ccggagggcg gcctggagga gcccgacgcc
      121 ctggatgaca gcctgaccag cctgcagtgg ctgcaggaat tctccattct caacgccaag
      181 gcccccgccc tgcccccggg gggcaccgac ccccacggct accaccaggt gccaggttca
      241 gcggcgcccg ggtcccccct ggcggccgac cccgcctgcc tggggcagcc acacacgccg
      301 ggcaagccca cgtcgtcgtg cacgtcgcgg agcgcgcccc cggggctgca ggccccaccc
      361 cccgacgacg tggactacgc caccaatccg cacgtgaagc ctccctactc gtatgccacg
      421 ctcatctgca tggccatgca ggccagcaag gccaccaaga tcaccctgtc ggccatctac
      481 aagtggatca cggacaactt ctgctacttc cgccacgcag atcccacctg gcagaattca
      541 atccgccaca acctgtctct gaacaagtgc ttcatcaaag tgcctcggga gaaggacgaa
      601 ccaggcaagg ggggcttctg gcgcattgac ccccagtacg cggagcggct actgagcggc
      661 gctttcaaga agcggcgact gccccctgtc cacatccacc cagcctttgc ccgccaggcc
      721 gcgcaggagc ccagcgctgt cccccgggcc gggccgctga cggtgaatac cgaggcccag
      781 cagctgctgc gggagttcga ggaggccacc ggggaggcgg gctggggtgc aggcgagggc
      841 aggctggggc ataagcgcaa acagccgctg cccaagcggg tggccaaggt cccgcggccc
      901 cccagcaccc tgctgcccac cccggaggag cagggtgagc tggaacccct caaaggcaac
      961 tttgactggg aggccatctt cgacgccggc actctgggcg gggagctggg tgcactggag
     1021 gccctggagc tgagcccgcc tctgagcccc gcctcacacg tggacgtgga cctcaccatc
     1081 cacggccgcc acatcgactg ccctgccacc tgggggcctt cggtggagca ggctgccgac
     1141 agcctggact tcgatgagac cttcctggcc acatccttcc tgcagcaccc ctgggacgag
     1201 agcggcagtg gctgcctgcc cccggagccc ctctttgagg ctggggatgc caccctggcc
     1261 tccgacctgc aggactgggc cagcgtgggg gccttcttgt acccaacttt cttgtacaaa
     1321 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1381 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1441 gacgaaaaat gctcccgtcc gttgggcca
//
