LOCUS       sequence-286341-        1583 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3266
FEATURES             Location/Qualifiers
     source          1..1583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1415..1439)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1447..1488
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgg cagccccctt ggaggcccag
       61 gaccaggccc ctggggaggg agaagggctt ctgattgtga aagtggaaga ttcctcctgg
      121 gaacaggaat ctgcccagca tgaggatggc agggattccg aagcctgccg ccagcgcttc
      181 cggcaattct gctacgggga tgtgcatggg cctcatgagg ccttcagcca gctctgggag
      241 ctctgctgcc gctggctgcg gcccgagctg cgtaccaagg agcagatcct ggagctgctg
      301 gtgctggagc agttcctgac agtgctgcca ggggagatcc agggctgggt gcgtgagcag
      361 cacccgggaa gcggtgagga ggctgtcgcc ttggtggagg acctacagaa gcagccagtg
      421 aaagcctggc gacaggatgt gccctcggag gaggcggaac ccgaggctgc aggccgggga
      481 tcccaggcca cggggcctcc cccgacggtg ggggcacgga ggcggccgtc tgttccccag
      541 gagcagcaca gccatagcgc ccagcctcct gctcttctta aagagggtcg tcccggagag
      601 acgacggaca cctgctttgt ctctggggtc catggacctg tggcattggg agacatccca
      661 ttctatttct cccgggaaga atggggcacc ctggaccctg ctcagcggga tctcttctgg
      721 gacataaagc gggagaactc ccggaacacc accctgggtt ttgggctcaa aggccaaagt
      781 gagaagtccc tgctgcagga gatggtgccg gtggtgccag gccagacagg cagcgacgtg
      841 actgtgtcct ggagccccga ggaggctgag gcctgggaga gcgagaaccg gccgagggcg
      901 gccctgggcc cagtggtggg cgcgcgacgg gggcggccac ccactcgccg gcgccagttc
      961 cgggacctgg cagccgagaa gccgcacagc tgcgggcagt gtggaaagcg cttccgctgg
     1021 ggctcggacc tggcgcggca ccagcgcacg cacacgggcg agaagccaca caagtgccct
     1081 gagtgcgaca agagcttccg cagctcctcg gacctggtgc gccaccaagg cgtgcacacg
     1141 ggcgagaagc ccttctcctg ttccgagtgc ggcaagagct tcagccgcag cgcctacctg
     1201 gccgaccacc agcgcataca cacgggcgag aagcctttcg gctgcagcga ctgcggcaag
     1261 agcttctcgc tgcgctccta cctgctggac catcggcgtg tgcacaccgg tgagcggccc
     1321 ttcggctgcg gagagtgcga caagagcttc aagcagcgcg cgcacctcat cgcgcatcag
     1381 agcctgcacg ccaagatggc ccagcccgtg gggtgcccaa ctttcttgta caaagtggtt
     1441 gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgaact
     1501 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     1561 accaacccga cgaacatgcg ata
//
