LOCUS       sequence-286371-        1556 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3276
FEATURES             Location/Qualifiers
     source          1..1556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1388..1412)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1420..1461
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggagttttt catcagtatg
       61 tctgaaacca ttaaatataa tgacgatgat cataaaactc tgtttctgaa aacactaaat
      121 gaacaacgcc tggaaggaga attttgtgat attgctattg tggttgagga tgtgaaattc
      181 agagcacaca gatgtgttct tgctgcctgc agcacctact ttaaaaagct tttcaagaag
      241 cttgaggttg atagttcttc ggtcatagaa atagattttc ttcgttctga tatatttgaa
      301 gaggtcctga actacatgta cacagcaaag atttccgtga aaaaagaaga tgttaactta
      361 atgatgtcat cgggtcagat tcttggtatc cgatttttgg ataaactgtg ttctcagaag
      421 cgtgatgtgt ccagtcccga tgaaaacaat ggtcagtcca aaagtaagta ttgccttaaa
      481 ataaatcgcc ccattggaga tgctgctgac acccaggatg atgatgtaga ggaaatcggg
      541 gatcaggatg acagtccttc tgatgacaca gtagaaggca cacccccgag tcaggaggac
      601 ggcaagtcgc ccaccacaac gctcagggtt caggaagcga tcctgaaaga gctggggagt
      661 gaggaagttc ggaaggtcaa ttgctacggc caggaagtag aatccatgga gaccccagaa
      721 tcaaaagact tggggtccca gacccctcaa gccttaacat ttaatgatgg gatgagtgaa
      781 gtgaaagatg aacagacacc aggctggaca acagccgcca gtgacatgaa gtttgagtat
      841 ttgctttatg gtcaccatcg ggagcagatt gcctgccagg cgtgtgggaa gacgttttct
      901 gatgaaggca gattgaggaa gcatgagaaa ctccacacgg cggacaggcc atttgtttgt
      961 gaaatgtgca caaaaggttt caccacacag gcccacctga aagaacacct aaaaatccac
     1021 acaggatata agccctatag ctgtgaggtg tgtggaaaat catttatccg tgccccagac
     1081 ttaaagaagc atgagagagt tcacagtaat gaaagaccgt ttgcgtgcca catgtgtgac
     1141 aaagccttca aacacaagtc tcacctcaag gatcatgaaa gaagacacag aggggaaaag
     1201 ccttttgtgt gtggctcctg caccaaggca tttgccaagg catctgatct gaaaaggcac
     1261 gagaacaata tgcacagtga aaggaagcag gttaccccca gtgccatcca gagcgagaca
     1321 gaacagttgc aggcggcagc gatggctgcg gaagcagaac agcagctgga gacgatagcc
     1381 tgtagcttgc caactttctt gtacaaagtg gttgatatcg gtaagcctat ccctaaccct
     1441 ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1501 tcttggcttt atatatcttg tggaaaggac gaagcccgca atcggtgtaa tccgaa
//
