LOCUS       sequence-286434-        2690 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3297
FEATURES             Location/Qualifiers
     source          1..2690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2522..2546)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2554..2595
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tggagggcgc cggcggcgcg
       61 aacgacaaga aaaagataag ttctgaacgt cgaaaagaaa agtctcgaga tgcagccaga
      121 tctcggcgaa gtaaagaatc tgaagttttt tatgagcttg ctcatcagtt gccacttcca
      181 cataatgtga gttcgcatct tgataaggcc tctgtgatga ggcttaccat cagctatttg
      241 cgtgtgagga aacttctgga tgctggtgat ttggatattg aagatgacat gaaagcacag
      301 atgaattgct tttatttgaa agccttggat ggttttgtta tggttctcac agatgatggt
      361 gacatgattt acatttctga taatgtgaac aaatacatgg gattaactca gtttgaacta
      421 actggacaca gtgtgtttga ttttactcat ccatgtgacc atgaggaaat gagagaaatg
      481 cttacacaca gaaatggcct tgtgaaaaag ggtaaagaac aaaacacaca gcgaagcttt
      541 tttctcagaa tgaagtgtac cctaactagc cgaggaagaa ctatgaacat aaagtctgca
      601 acatggaagg tattgcactg cacaggccac attcacgtat atgataccaa cagtaaccaa
      661 cctcagtgtg ggtataagaa accacctatg acctgcttgg tgctgatttg tgaacccatt
      721 cctcacccat caaatattga aattccttta gatagcaaga ctttcctcag tcgacacagc
      781 ctggatatga aattttctta ttgtgatgaa agaattaccg aattgatggg atatgagcca
      841 gaagaacttt taggccgctc aatttatgaa tattatcatg ctttggactc tgatcatctg
      901 accaaaactc atcatgatat gtttactaaa ggacaagtca ccacaggaca gtacaggatg
      961 cttgccaaaa gaggtggata tgtctgggtt gaaactcaag caactgtcat atataacacc
     1021 aagaattctc aaccacagtg cattgtatgt gtgaattacg ttgtgagtgg tattattcag
     1081 cacgacttga ttttctccct tcaacaaaca gaatgtgtcc ttaaaccggt tgaatcttca
     1141 gatatgaaaa tgactcagct attcaccaaa gttgaatcag aagatacaag tagcctcttt
     1201 gacaaactta agaaggaacc tgatgcttta actttgctgg ccccagccgc tggagacaca
     1261 atcatatctt tagattttgg cagcaacgac acagaaactg atgaccagca acttgaggaa
     1321 gtaccattat ataatgatgt aatgctcccc tcacccaacg aaaaattaca gaatataaat
     1381 ttggcaatgt ctccattacc caccgctgaa acgccaaagc cacttcgaag tagtgctgac
     1441 cctgcactca atcaagaagt tgcattaaaa ttagaaccaa atccagagtc actggaactt
     1501 tcttttacca tgccccagat tcaggatcag acacctagtc cttccgatgg aagcactaga
     1561 caaagttcac ctgagcctaa tagtcccagt gaatattgtt tttatgtgga tagtgatatg
     1621 gtcaatgaat tcaagttgga attggtagaa aaactttttg ctgaagacac agaagcaaag
     1681 aacccatttt ctactcagga cacagattta gacttggaga tgttagctcc ctatatccca
     1741 atggatgatg acttccagtt acgttccttc gatcagttgt caccattaga aagcagttcc
     1801 gcaagccctg aaagcgcaag tcctcaaagc acagttacag tattccagca gactcaaata
     1861 caagaaccta ctgctaatgc caccactacc actgccacca ctgatgaatt aaaaacagtg
     1921 acaaaagacc gtatggaaga cattaaaata ttgattgcat ctccatctcc tacccacata
     1981 cataaagaaa ctactagtgc cacatcatca ccatatagag atactcaaag tcggacagcc
     2041 tcaccaaaca gagcaggaaa aggagtcata gaacagacag aaaaatctca tccaagaagc
     2101 cctaacgtgt tatctgtcgc tttgagtcaa agaactacag ttcctgagga agaactaaat
     2161 ccaaagatac tagctttgca gaatgctcag agaaagcgaa aaatggaaca tgatggttca
     2221 ctttttcaag cagtaggaat tggaacatta ttacagcagc cagacgatca tgcagctact
     2281 acatcacttt cttggaaacg tgtaaaagga tgcaaatcta gtgaacagaa tggaatggag
     2341 caaaagacaa ttattttaat accctctgat ttagcatgta gactgctggg gcaatcaatg
     2401 gatgaaagtg gattaccaca gctgaccagt tatgattgtg aagttaatgc tcctatacaa
     2461 ggcagcagaa acctactgca gggtgaagaa ttactcagag ctttggatca agttaactga
     2521 gacccagctt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     2581 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     2641 ctttatatat cttgtggaaa ggacgagggc ccgctgcgcc atcccgcctt
//
