LOCUS       sequence-286446-        1022 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3301
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(854..878)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             886..927
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga attctgattc gagctctgtc
       61 tccagcagag cttcatctcc ggacatggat gagatgtacc tgagggacca ccaccaccgc
      121 caccaccacc accaggagag ccgtctcaac tcggtctcgt ccacgcaggg cgatatgatg
      181 cagaagatgc ccggggaaag cctctcgcgg gctggcgcca aggccgcggg agagagcagc
      241 aagtacaaaa tcaagaagca gctgtcggag caggacctac agcagttgag gctgaagatc
      301 aacggacgcg aacgcaagcg gatgcacgac ctgaacctag ccatggacgg gctgcgcgaa
      361 gtcatgccct acgcgcatgg gccgtcggtg cgcaagctct ccaagatcgc cacactcctg
      421 ctcgccagaa actacatcct catgctcacc agctccctgg aggagatgaa gaggctggtt
      481 ggcgagatct atgggggcca ccactcggcc tttcactgcg ggaccgtggg ccactcggcc
      541 ggccaccccg cgcacgcggc caactccgtg cacccggtgc accccatctt gggcggcgcg
      601 ctctcatctg gcaacgcctc gtcaccgctg tccgccgcct cacttcccgc catcggcacc
      661 atccggcctc cccactcgct actcaaggcg ccctccacgc cgcccgcgct gcagctgggc
      721 agcggcttcc agcactgggc tggtctgccc tgcccctgca ccatctgcca gatgccgccg
      781 ccgccgcacc tgtccgctct ctccacagcc aacatggccc ggctgtcggc cgagtccaag
      841 gacttgctca agtgcccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
      901 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
      961 tcgatttctt ggctttatat atcttgtgga aaggacgact gtggagcgca caggacggcg
     1021 cc
//
