LOCUS       sequence-286539-        1754 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3332
FEATURES             Location/Qualifiers
     source          1..1754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1586..1610)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1618..1659
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttagcatgg agaacgggca gagcacagcc
       61 gccaagctgg ggctgcctcc cctgacgccc gagcagcagg aggcccttca gaaggccaag
      121 aagtacgcca tggagcagag catcaagagt gtgctggtga agcagaccat cgcgcaccag
      181 cagcagcagc tcaccaacct gcagatggca gcagtgacaa tgggctttgg agatcctctc
      241 tcacctttgc aatcgatggc ggctcagcgg cagcgggcgc tggccatcat gtgccgcgtc
      301 tacgtgggct ctatctacta tgagctgggg gaggacacca tccgccaggc ctttgccccc
      361 tttggcccca tcaagagcat cgacatgtcc tgggactccg tcaccatgaa gcacaagggc
      421 tttgccttcg tggagtatga ggtccccgaa gctgcacagc tggccttgga gcagatgaac
      481 tcggtgatgc tggggggcag gaacatcaag gtgggcagac ccagcaacat agggcaggcc
      541 cagcccatca tagaccagtt ggctgaggag gcacgggcct tcaaccgcat ctacgtggcc
      601 tctgtgcacc aggacctctc agacgatgac atcaagagcg tgtttgaggc ctttggcaag
      661 atcaagtcct gcacactggc ccgggacccc acaactggca agcacaaggg ctacggcttc
      721 attgagtacg agaaggccca gtcgtcccaa gatgctgtgt cttccatgaa cctctttgac
      781 ctgggtggcc agtacttgcg ggtgggcaag gctgtcacac cgcccatgcc cctactcaca
      841 ccagccacgc ctggaggcct cccacctgcc gctgctgtgg cagctgctgc agccactgcc
      901 aagatcacag ctcaggaagc agtggccgga gcagcggtgc tgggtaccct gggcacacct
      961 ggactggtgt ccccagcact gaccctggcc cagcccctgg gcactttgcc ccaggctgtc
     1021 atggctgccc aggcacctgg agtcatcaca ggtgtgaccc cagcccgtcc tcctatcccg
     1081 gtcaccatcc cctcggtggg agtggtgaac cccatcctgg ccagccctcc aacgctgggt
     1141 ctcctggagc ccaagaagga gaaggaagaa gaggagctgt ttcccgagtc agagcggcca
     1201 gagatgctga gcgagcagga gcacatgagc atctcgggca gtagcgcccg acacatggtg
     1261 atgcagaagc tgctccgcaa gcaggagtct acagtgatgg ttctgcgcaa catggtggac
     1321 cccaaggaca tcgatgatga cctggaaggg gaggtgacag aggagtgtgg caagttcggg
     1381 gccgtgaacc gcgtcatcat ctaccaagag aaacaaggcg aggaggagga tgcagaaatc
     1441 attgtcaaga tctttgtgga gttttccata gcctctgaga ctcataaggc catccaggcc
     1501 ctcaatggcc gctggtttgc tggccgcaag gtggtggctg aagtgtacga ccaggagcgt
     1561 tttgataaca gtgacctctc tgcgtgccca actttcttgt acaaagtggt tgatatcggt
     1621 aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgaac tagtccgtaa
     1681 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga taggtctact
     1741 tataatctgc acga
//
