LOCUS       sequence-286569-        3227 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3342
FEATURES             Location/Qualifiers
     source          1..3227
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3059..3083)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3091..3132
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gcaggcacca tggatctgac tcttgctgaa
       61 cttcaggaaa tggcatctcg ccagcagcaa cagattgaag cccagcaaca attgctggca
      121 actaaggaac agcgcttaaa gtttttgaaa caacaagatc agcgacaaca gcaacaagtt
      181 gctgagcagg agaaacttaa aaggctaaaa gaaatagctg agaatcagga agctaagcta
      241 aaaaaagtga gagcacttaa aggccacgtg gaacagaaga gactaagcaa tgggaaactt
      301 gtggaggaaa ttgaacagat gaataatttg ttccagcaaa aacagaggga gctcgtcctg
      361 gctgtgtcaa aagtagaaga actgaccagg cagctagaga tgctcaagaa cggcaggatc
      421 gacagccacc atgacaatca gtctgcagtg gctgagcttg atcgcctcta taaggagctg
      481 cagctaagaa acaaattgaa tcaagagcag aatgccaagc tacaacaaca gagggagtgt
      541 ttgaataagc gtaattcaga agtggcagtc atggataagc gtgttaatga gctgagggac
      601 cggctgtgga agaagaaggc agctctacag caaaaagaaa atctaccagt ttcatctgat
      661 ggaaatcttc cccagcaagc cgcgtcagcc ccaagccgtg tggctgcagt aggtccctat
      721 atccagtcgt ctactatgcc tcggatgccc tcaaggcctg aattgctggt gaagccagcc
      781 ctgccggatg gttccttggt cattcaggct tcagaggggc cgatgaaaat acagacactg
      841 cccaacatga gatctggggc tgcttcacaa actaaaggct ctaaaatcca tccagttggc
      901 cctgattgga gtccttcaaa tgcagatctt ttcccaagcc aaggctctgc ttctgtacct
      961 caaagcactg ggaatgctct ggatcaagtt gatgatggag aggttccgct gagggagaaa
     1021 gagaagaaag tgcgtccgtt ctcaatgttt gatgcagtag accagtccaa tgccccacct
     1081 tcctttggta ctctgaggaa gaaccagagc agtgaagata tcttgcggga tgctcaggtt
     1141 gcaaataaaa atgtggctaa agtaccacct cctgttccta caaaaccaaa acagattaat
     1201 ttgccttatt ttggacaaac taatcagcca ccttcagaca ttaagccaga cggaagttct
     1261 cagcagttgt caacagttgt tccgtccatg ggaactaaac caaaaccagc agggcagcag
     1321 ccgagagtgc tgctatctcc cagcatacct tcggttggcc aagaccagac cctttctcca
     1381 ggttctaagc aagaaagtcc acctgctgct gccgtccggc cctttactcc ccagccttcc
     1441 aaagacacct tacttccacc cttcagaaaa ccccagaccg tggcagcaag ttcaatatat
     1501 tccatgtata cgcaacagca ggcgccagga aaaaacttcc agcaggctgt gcagagcgcg
     1561 ttgaccaaga ctcataccag agggccacac ttttcaagtg tatatggtaa gcctgtaatt
     1621 gctgctgccc agaatcaaca gcagcaccca gagaacattt attccaatag ccagggcaag
     1681 cctggcagtc cagaacctga aacagagcct gtttcttcag ttcaggagaa ccatgaaaac
     1741 gaaagaattc ctcggccact cagcccaact aaattactgc ctttcttatc taatccttac
     1801 cgaaaccaga gtgatgctga cctagaagcc ttacgaaaga aactgtctaa cgcaccaagg
     1861 cctctaaaga aacgtagttc tattacagag ccagagggtc ctaatgggcc aaatattcag
     1921 aagcttttat atcagaggac caccatagcg gccatggaga ccatctctgt cccatcatac
     1981 ccatccaagt cagcttctgt gactgccagc tcagaaagcc cagtagaaat ccagaatcca
     2041 tatttacatg tggagcccga aaaggaggtg gtctctctgg ttcctgaatc attgtcccca
     2101 gaggatgtgg ggaatgccag tacagagaac agtgacatgc cagctccttc tccaggcctt
     2161 gattatgagc ctgagggagt cccagacaac agcccaaatc tccagaataa cccagaagaa
     2221 ccaaatccag aggctccaca tgtgcttgat gtgtacctgg aggagtaccc tccataccca
     2281 cccccaccat acccatctgg ggagcctgaa gggcccggag aagactcggt gagcatgcgc
     2341 ccgcctgaaa tcaccgggca ggtctctctg cctcctggta aaaggacaaa cttgcgtaaa
     2401 actggctcag agcgtatcgc tcatggaatg agggtgaaat tcaaccccct tgctttactg
     2461 ctagattcgt ctttggaggg agaatttgac cttgtacaga gaattattta tgaggttgat
     2521 gacccaagcc tccccaatga tgaaggcatc acggctcttc acaatgctgt gtgtgcaggc
     2581 cacacagaaa tcgttaagtt cctggtacag tttggtgtaa atgtaaatgc tgctgatagt
     2641 gatggatgga ctccattaca ttgtgctgcc tcatgtaaca acgtccaagt gtgtaagttt
     2701 ttggtggagt caggagccgc tgtgtttgcc atgacctaca gtgacatgca gactgctgca
     2761 gataagtgcg aggaaatgga ggaaggctac actcagtgct cccaatttct ttatggagtt
     2821 caggagaaga tgggcataat gaataaagga gtcatttatg cgctttggga ttatgaacct
     2881 cagaatgatg atgagctgcc catgaaagaa ggagactgca tgacaatcat ccacagggaa
     2941 gacgaagatg aaatcgaatg gtggtgggcg cgccttaatg ataaggaggg atatgttcca
     3001 cgtaacttgc tgggactgta cccaagaatt aaaccaagac aaaggagctt ggcctgagac
     3061 ccagctttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     3121 ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     3181 tatatatctt gtggaaagga cgacccgcac gcaaactaca ttcgtca
//
