LOCUS       sequence-286647-        1085 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3368
FEATURES             Location/Qualifiers
     source          1..1085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(917..941)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             949..990
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggggaacga gctctttctg
       61 gccttcacca catctcacct ccccttagcg gagcagaaac ttgccaggta taaactccga
      121 attgttaagc caccaaaatt acccctagag aaaaaaccca accctgataa ggatggtcca
      181 gattatgagc ccaacctctg gatgtgggta aatcccaaca ttgtgtatcc ccctggaaag
      241 ctggaggtct caggacgtag gaagagggag gacctgacaa gcacactccc ctcctctcag
      301 ccaccccaga aggaggaaga tgccagctgc tcagaggccg caggggtgga atcactgtcc
      361 cagtcctcca gcaagcggtc tccccctcgg aagcggtttg ccttttcccc cagcacctgg
      421 gagctcacag aagaggagga ggctgaggac caggaagaca gctcctctat ggctctccca
      481 tcccctcaca aaagggcccc cctccagagt cggaggcttc ggcaagccag cagccaggcg
      541 gggaggctct ggtcccggcc ccctctcaat tacttccacc taattgccct ggcattaaga
      601 aacagttccc cctgtggcct caacgtgcaa cagatctaca gtttcactcg aaagcacttc
      661 ccctttttcc ggacggcccc ggaaggctgg aagaatactg tccgtcacaa tctctgtttt
      721 cgagacagct ttgagaaagt gcctgtcagc atgcagggcg gggccagcac acggcctcga
      781 tcttgcctct ggaagttgac cgaggaggga caccgccgct ttgcggagga ggcccgcgcc
      841 ttggcttcca ctcggctaga aagtatccaa cagtgcatga gccagccaga tgtgatgccc
      901 ttcctctttg atcttttgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
      961 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1021 atttcgattt cttggcttta tatatcttgt ggaaaggacg atgcttaaaa ctaacacaaa
     1081 cagaa
//
