LOCUS       sequence-286695-        1232 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3384
FEATURES             Location/Qualifiers
     source          1..1232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1064..1088)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1096..1137
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga catgccctcg caatgtaact
       61 ccgaactcgt acgcggagcc cttggctgcg cccggcggag gagagcgcta tagccggagc
      121 gcaggcatgt atatgcagtc tgggagtgac ttcaattgcg gggtgatgag gggctgcggg
      181 ctcgcgccct cgctctccaa gagggacgag ggcagcagcc ccagcctcgc cctcaacacc
      241 tatccgtcct acctctcgca gctggactcc tggggcgacc ccaaagccgc ctatcgcctg
      301 gaacaacctg ttggcaggcc gctgtcctcc tgctcctacc cacctagtgt caaggaggag
      361 aatgtctgct gcatgtacag cgcagagaag cgggcgaaaa gtggccccga ggcagctctc
      421 tactcccacc ccttgccgga gtcctgcctt ggggagcacg aggtacccgt gcccagctac
      481 taccgcgcca gcccgagcta ctccgcgctg gacaagacgc cccactgttc tggggccaac
      541 gacttcgaag cccctttcga gcagcgggcc agtctcaacc cgcgcgccga acatctggaa
      601 tcgcctcagc tggggggcaa agtgagtttc cctgagaccc ccaagtccga cagccagacc
      661 cccagcccca atgaaatcaa gacggagcag agcctggcgg gccctaaagg gagcccctcg
      721 gagagcgaaa aggagagggc caaagctgcc gactccagcc cagacacctc ggataacgaa
      781 gcgaaagagg agataaaggc agaaaacacc acaggaaatt ggctgacagc aaagagcgga
      841 aggaagaaga ggtgccccta tactaaacac cagacgctgg aattggagaa agaatttctg
      901 ttcaatatgt atttgacgcg agagcgccgc ctggagatta gcaagaccat taaccttaca
      961 gacagacaag tcaaaatctg gtttcaaaat cgcagaatga aactcaagaa aatgaaccga
     1021 gagaatcgga tccgggaact gacctccaat tttaatttca cctgcccaac tttcttgtac
     1081 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1141 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1201 aaggacgaaa ttgtcgtcta gacatagcaa gc
//
