LOCUS       sequence-286875-         962 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3444
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(794..818)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             826..867
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc ggggccaggg ccgcaaggag
       61 agtttgtccg attcccgaga cctggacggc tcctacgacc agctcacggg ccaccctcca
      121 gggcccacta aaaaagcgct gaagcagcga ttcctcaagc tgctgccgtg ctgcgggccc
      181 caagccctgc cctcagtcag tgaaaacagc gtggacgatg aatttgaatt gtccaccgtg
      241 tgtcaccggc ctgagggtct ggagcagctg caggagcaaa ccaaattcac gcgcaaggag
      301 ttgcaggtcc tgtaccgggg cttcaagaac gaatgtccca gcggaattgt caatgaggag
      361 aacttcaagc agatttactc ccagttcttt cctcaaggag actccagcac ctatgccact
      421 tttctcttca atgcctttga caccaaccat gatggctcgg tcagttttga ggactttgtg
      481 gctggtttgt ccgtgattct tcggggaact gtagatgaca ggcttaattg ggccttcaac
      541 ctgtatgacc ttaacaagga cggctgcatc accaaggagg aaatgcttga catcatgaag
      601 tccatctatg acatgatggg caagtacacg taccctgcac tccgggagga ggccccaagg
      661 gaacacgtgg agagcttctt ccagaagatg gacagaaaca aggatggtgt ggtgaccatt
      721 gaggaattca ttgagtcttg tcaaaaggat gagaacatca tgaggtccat gcagctcttt
      781 gacaatgtca tctacccaac tttcttgtac aaagtggttg atatcggtaa gcctatccct
      841 aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact tgaaagtatt
      901 tcgatttctt ggctttatat atcttgtgga aaggacgatc cacaccaaat ttcaataggc
      961 gc
//
