LOCUS       sequence-286932-        1229 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3463
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1061..1085)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1093..1134
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc aggagggaga attggctatt
       61 tctcctataa gccctgtggc agccatgcct cccctaggca cccacgtgca agccagatgt
      121 gaagctcaaa ttaacctgct gggtgaaggg gggatctgca agctgccagg aagactccgc
      181 atccagcccg cactgtggag cagggaggac gtgctgcact ggctgcgctg ggcagagcag
      241 gagtactctc tgccatgcac cgcggagcac gggttcgaga tgaacggacg cgccctctgc
      301 atcctcacca aggacgactt ccggcaccgt gcgcccagct caggtgacgt cctgtatgag
      361 ctgctccagt acatcaagac ccagcggcga gccctggtgt gtggaccctt ttttggaggg
      421 atcttcaggc tgaagacgcc cacccagcac tctccagtcc ccccggaaga ggtgactggc
      481 ccctctcaga tggacacccg aaggggccac ctgctgcagc caccagaccc agggcttacc
      541 agcaacttcg gccacctgga tgaccctggc ctggcaaggt ggacccctgg caaggaggag
      601 tccctcaact tatgtcactg tgcagagctc ggctgcagga cccagggggt ctgttccttc
      661 cccgcgatgc cgcaggcccc cattgacggc aggatcgctg actgccgcct gctgtgggat
      721 tacgtgtatc agctgctcct tgatacccga tatgagccct acatcaagtg ggaagacaag
      781 gacgccaaga tcttccgagt tgtggatcca aatgggctcg ccagactctg gggaaatcac
      841 aagaaccggg tgaacatgac ctacgagaag atgtctcgtg ccctgcgcca ctattataag
      901 cttaatatca ttaagaagga accggggcag aaactcctgt tcagatttct aaagactccg
      961 ggaaagatgg tccaggacaa gcacagccac ctggagccgc tggagagcca ggagcaggac
     1021 agaatagagt tcaaggacaa gaggccagaa atctctccgt gcccaacttt cttgtacaaa
     1081 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     1141 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1201 gacgatgaat aaagtgcctt tagtgcacg
//
