LOCUS       sequence-287286-        1632 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses mScarlet-MYO10 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Jacquemet G, Stubb A, Saup R, Miihkinen M, Kremneva E, Hamidi H, 
            Ivaska J
  TITLE     Filopodome Mapping Identifies p130Cas as a Mechanosensitive 
            Regulator of Filopodia Stability.
  JOURNAL   Curr Biol. 2019 Jan 21;29(2):202-216.e7. doi: 
            10.1016/j.cub.2018.11.053. Epub 2019 Jan 10.
  PUBMED    30639111
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2018.11.053"; journalName: "Curr Biol"; date: 
            "2019-01-21- 21"; volume: "29"; issue: "2"; pages: "202-216.e7"
COMMENT     Sequence Label: mScarlet-MYO10
FEATURES             Location/Qualifiers
     source          1..1632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..113
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             117..149
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(153..161,162..176)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             194..889
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(327..345)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     595..614
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             905..931
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             950..967
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      1231..1240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1355..1364
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
       61 tagcgtttaa acttaagctt accatggggg gttctcatca tcatcatcat catggtatgg
      121 ctagcatgac tggtggacag caaatgggtc gggatctgta cgacgatgac gataaggtac
      181 caatgttact agtatggtga gcaagggcga ggcagtgatc aaggagttca tgcggttcaa
      241 ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      301 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
      361 cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct tcaccaagca
      421 ccccgccgac atccccgact actataagca gtccttcccc gagggcttca agtgggagcg
      481 cgtgatgaac ttcgaggacg gcggcgccgt gaccgtgacc caggacacct ccctggagga
      541 cggcaccctg atctacaagg tgaagctccg cggcaccaac ttccctcctg acggccccgt
      601 aatgcagaag aagacaatgg gctgggaagc gtccaccgag cggttgtacc ccgaggacgg
      661 cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc gctacctggc
      721 ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg cctacaacgt
      781 cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg aacagtacga
      841 acgctccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt ccggactcag
      901 atcctacccc tatgatgtgc ctgactatgc cggacacgga cacggccacc tggtgcccag
      961 gggcagcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtacca tggataactt
     1021 cttcaccgag ggaacacggg tctggctgag agaaaatggc cagcattttc caagtactgt
     1081 aaattcctgt gcagaaggca tcgtcgtctt ccggacagac tatggtcagg tattcactta
     1141 caagcagagc acaattaccc accagaaggt gactgctatg caccccacga acgaggaggg
     1201 cgtggatgac atggcgtcct tgacagagct ccatggcggc tccatcatgt ataacttatt
     1261 ccagcggtat aagagaaatc aaatatatac ctacatcggc tccatcctgg cctccgtgaa
     1321 cccctaccag cccatcgccg ggctgtacga gcctgccacc atggagcagt acagccggcg
     1381 ccacctgggc gagctgcccc cgcacatctt cgccatcgcc aacgagtgct accgctgcct
     1441 gtggaagcgc tacgacaacc agtgcatcct catcagtggt gaaagtgggg caggtaaaac
     1501 cgaaagcact aaattgatcc tcaagtttct gtcagtcatc agtcaacagt ctttggaatt
     1561 gtccttaaag gagaagacat cctgtgttga acgagctatt cttgaaagca gccccatcat
     1621 ggaagctttc gg
//
