LOCUS       sequence-28754-e         562 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Lin CW, Jao CY, Ting AY
  TITLE     Genetically encoded fluorescent reporters of histone methylation in 
            living cells.
  JOURNAL   J Am Chem Soc. 2004 May 19. 126(19):5982-3.
  PUBMED    15137760
REFERENCE   2  (bases 1 to 562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2004 May 19. 126(19):5982-3."
COMMENT     Sequence Label: pcDNA3-K9 histone methylation reporter
FEATURES             Location/Qualifiers
     source          1..562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..325)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(116..137)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     465..484
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gaactataga atagggccct ctagatgcat gctcgagcgg ccgccagtgt gatggatatc
       61 tgcagaattc ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      121 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggt
      181 agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
      241 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
      301 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
      361 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
      421 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
      481 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
      541 agtcgtgctg cttcatgtgg tc
//
