LOCUS       sequence-288105-        3736 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway-compatible Entry vector, with insert of ORF7A gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 ORF7A
FEATURES             Location/Qualifiers
     source          1..3736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(110..137)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(229..315)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    368..467
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(834..933)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1045..1064)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1123..1142)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1716..2249)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2438..2443,2444..2460,2461..2466))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3123..3711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3612..3631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
       61 ctgtggataa ccgtattacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
      121 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
      181 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
      241 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
      301 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatct
      361 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
      421 tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gttggcatga agatcatcct
      481 gttcctggcc ctgatcaccc tggctacctg cgagctgtac cactaccagg aatgcgtgcg
      541 cggtaccacc gtgctgctga aggagccttg cagctccggc acctacgaag gaaactcccc
      601 cttccaccct ctggccgaca acaagttcgc tctgacctgc ttctctactc agttcgcctt
      661 cgcttgccct gatggtgtga agcacgtgta ccagctgcgc gccaggtctg tgagcccaaa
      721 gctgttcatc agacaggagg aagtgcagga gctgtacagc cccatcttcc tgatcgtggc
      781 cgctatcgtg ttcatcaccc tgtgcttcac cctgaagcgt aagaccgaat aatacccaac
      841 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
      901 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgcagctc tggcccgtgt
      961 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
     1021 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
     1081 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     1141 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag
     1201 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     1261 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     1321 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag
     1381 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     1441 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
     1501 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     1561 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
     1621 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     1681 taggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact tgggtcgata
     1741 tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac tgcgggatcg
     1801 tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg
     1861 aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga gactgcgaga
     1921 tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta agccgcgaga
     1981 gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt actacggagc
     2041 aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac gtctccgaac
     2101 tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg gccgagccta
     2161 catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg actgccctgc
     2221 tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     2281 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc
     2341 ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
     2401 ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta
     2461 tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg
     2521 cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg tttcggtctc
     2581 cacgcatcgt caggcattgg cggccttgct gttcttctac ggcaaggtgc tgtgcacgga
     2641 tctgccctgg cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc cggtggtgct
     2701 gaccccggat gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc
     2761 ccaggactct agctatagtt ctagtggttg gctactaata ggttgtattg atgttggacg
     2821 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     2881 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     2941 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt
     3001 gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc
     3061 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3121 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3181 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3241 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3301 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3361 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3421 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3481 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3541 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3601 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3661 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3721 aacgcggcct ttttac
//
