LOCUS       sequence-288144-        8499 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Donor vector to knockin EGFP  (separated by 2A) into EPHB4 allele.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Zhang J, Chu LF, Hou Z, Schwartz MP, Hacker T, Vickerman V, Swanson 
            S, Leng N, Nguyen BK, Elwell A, Bolin J, Brown ME, Stewart R, 
            Burlingham WJ, Murphy WL, Thomson JA
  TITLE     Functional characterization of human pluripotent stem cell-derived 
            arterial endothelial cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jul 25;114(30):E6072-E6078. doi: 
            10.1073/pnas.1702295114. Epub 2017 Jul 10.
  PUBMED    28696312
REFERENCE   2  (bases 1 to 8499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1702295114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-07-25- 25"; volume: "114"; issue: "30"; pages: 
            "E6072-E6078"
COMMENT     Sequence Label: EPHB4-2A-EGFP
FEATURES             Location/Qualifiers
     source          1..8499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1203..1259
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1260..1262,1263..1265,1266..1979)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1260..1262,1263..1265,1266..1976)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1305..1326)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1566..1585)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1913..1934
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    2010..2043
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2104..2603
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2139..2162)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2536..2555
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2643..3242
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2643..2662)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    3799..3832
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4932..4951)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4933..4951)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4958..4975)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4958..4974)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4967..4989)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5115..5543
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5202..5221)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5412..5433
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             5887..6681
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5941..5960)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6551..6570
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(6699..6767,6768..7559)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6917..6936)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7730..8318
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8219..8238
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8472..8489
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccgag gtggaggttg cagtgagccg agatcgcacc actgcactcc
      301 agcctggatg acagagtgaa attccatctc aaaaaaaaaa aaaaaaaaaa agaaatgtga
      361 aggccaggtg gtggctcacg cctgtaatct cagcactttg ggaggctcag gtggaccgat
      421 tgcttgagcc caggagtttg agagcagcct ggccaaaata gcaaaacccc atctctacaa
      481 aacaaaaaca aaaaaattag ctgggcatgg tggtgcgtgc ctgtggtccc agctactcag
      541 gaggctagag ccagagggtc tcaggccagt ctgcccctgc cccacggggc ctgggcacat
      601 ccctccctaa ttcttcccag cctctctctg acccaggggg cctcctctcc cttttttccc
      661 cttatctcag cctccagcca tcagcaacct cctcttcctc tccacccagc tcttcctctc
      721 ccacttcggc cttttctttc tcacactcca tttccctcta cggcaatctg tgcagcctct
      781 tcccccagtc tcattttgcg ggcttttctc tcttttcttt ccttccctgg cacccaagcc
      841 aaaggccctg cctctggcct ccagccctac ccccttctgc ggttgcacag aaggatggct
      901 gcccagctct taaaaaaact gcccgggaac tgttgacatc tgttctccct cccccgctgg
      961 cttttctgat tggcttacaa tcctgaggct aggaccgtct caggagccaa gcgaggagag
     1021 cggccacagg gaacctaggg tctcaccaag ctctcctttc cttctgcagg gacctgctcc
     1081 gaatcggagt cactctggcg ggacaccaga agaaaatctt ggccagtgtc cagcacatga
     1141 agtcccaggc caagccggga accccgggtg ggacaggagg accggccccg cagtacgcta
     1201 gcgccactaa cttctccctg ttgaaacaag caggggatgt cgaagagaat cccgggccaa
     1261 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1321 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     1381 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1441 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     1501 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1561 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1621 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1681 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     1741 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     1801 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1861 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     1921 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     1981 aattccgatc atattcaata acccttaata taacttcgta taatgtatgc tatacgaagt
     2041 tattaggtct gaagaggagt ttacgtccag ccaagcttag gatctcgacc tcgaaattct
     2101 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     2161 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     2221 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     2281 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     2341 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     2401 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     2461 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     2521 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     2581 cttcctcatc tccgggcctt tcgacctgca tccatctaga tctcgagcag ctgaagctta
     2641 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     2701 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     2761 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     2821 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     2881 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     2941 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     3001 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     3061 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     3121 tggagacctc cgcgccccac aacctcccct tctacgagcg gctcggcttc accgtcaccg
     3181 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     3241 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga
     3301 tgatatcaga tccccgggat gcagaaattg atgatctatt aaacaataaa gatgtccact
     3361 aaaatggaag tttttcctgt catactttgt taagaagggt gagaacagag tacctacatt
     3421 ttgaatggaa ggattggagc tacgggggtg ggggtggggt gggattagat aaatgcctgc
     3481 tctttactga aggctcttta ctattgcttt atgataatgt ttcatagttg gatatcataa
     3541 tttaaacaag caaaaccaaa ttaagggcca gctcattcct cccactcatg atctatagat
     3601 ctatagatct ctcgtgggat cattgttttt ctcttgattc ccactttgtg gttctaagta
     3661 ctgtggtttc caaatgtgtc agtttcatag cctgaagaac gagatcagca gcctctgttc
     3721 cacatacact tcattctcag tattgttttg ccaagttcta attccatcag aagctggtcg
     3781 agatccggaa cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtccc
     3841 tcgaagaggt tcactaggcg cgcctgacct gcaggaactc cccaccccag ggacaccgcc
     3901 tccccatttt ccggggcaga gtggggactc acagaggccc ccagccctgt gccccgctgg
     3961 attgcacttt gagcccgtgg ggtgaggagt tggcaatttg gagagacagg atttgggggt
     4021 tctgccataa taggagggga aaatcacccc ccagccacct cggggaactc cagaccaagg
     4081 gtgagggcgc ctttccctca ggactgggtg tgaccagagg aaaaggaagt gcccaacatc
     4141 tcccagcctc cccaggtgcc cccctcacct tgatgggtgc gttcccgcag accaaagaga
     4201 gtgtgactcc cttgccagct ccagagtggg ggggctgtcc cagggggcaa gaaggggtgt
     4261 cagggcccag tgacaaaatc attggggttt gtagtcccaa cttgctgctg tcaccaccaa
     4321 actcaatcat ttttttccct tgtaaatgcc cctcccccag ctgctgcctt catattgaag
     4381 gtttttgagt tttgtttttg gtcttaattt ttctccccgt tccctttttg tttcttcgtt
     4441 ttgtttttct accgtccttg tcataacttt gtgttggagg gaacctgttt cactatggcc
     4501 tcctttgccc aagttgaaac aggggcccat catcatgtct gtttccagaa cagtgccttg
     4561 gtcatcccac atccccggac cccgcctggg acccccaagc tgtgtcctat gaaggggtgt
     4621 ggggtgaggt agtgaaaagg gcggtagttg gtggtggaac ccagaaacgg acgccggcgc
     4681 ttggaggggt tcttaaatta tatttaaaaa agtaactttt tgtataaata aaagaaaatg
     4741 ggacgtgtcc cagctccagg ggtgatgggg gtgatggact agatttctaa ggagagtggg
     4801 gccgggtagg gagggctttg tggctgaccg agaggtgtca gaggtctgga ggggccggcc
     4861 aaaagggcga attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagag
     4921 ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     4981 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     5041 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     5101 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     5161 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     5221 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     5281 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     5341 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     5401 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     5461 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     5521 ttaacaaaaa tttaacgcga attttaacaa aattcagggc gcaagggctg ctaaaggaag
     5581 cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac
     5641 tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg
     5701 cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca
     5761 gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg
     5821 ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg
     5881 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     5941 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     6001 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     6061 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     6121 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     6181 gggcaggatc tcctgtcatc ccaccttgct cctgccgaga aagtatccat catggctgat
     6241 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     6301 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     6361 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     6421 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     6481 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     6541 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     6601 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     6661 cttcttgacg agttcttctg aattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6721 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6781 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     6841 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     6901 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     6961 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7021 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7081 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7141 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7201 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7261 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7321 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7381 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7441 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7501 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     7561 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     7621 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     7681 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7741 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7801 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7861 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7921 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7981 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8041 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8101 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8161 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8221 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8281 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8341 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8401 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8461 cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
